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Q3SG32

- CYSG_THIDA

UniProt

Q3SG32 - CYSG_THIDA

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Protein
Siroheme synthase
Gene
cysG, Tbd_2471
Organism
Thiobacillus denitrificans (strain ATCC 25259)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei226 – 2261S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei249 – 2491Proton acceptor By similarity
Active sitei271 – 2711Proton donor By similarity
Binding sitei307 – 3071S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei384 – 3841S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei413 – 4131S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NAD By similarity
Nucleotide bindingi43 – 442NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTDEN292415:GHWG-2521-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Precorrin-2 dehydrogenase (EC:1.3.1.76)
Sirohydrochlorin ferrochelatase (EC:4.99.1.4)
Gene namesi
Name:cysG
Ordered Locus Names:Tbd_2471
OrganismiThiobacillus denitrificans (strain ATCC 25259)
Taxonomic identifieri292415 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaHydrogenophilalesHydrogenophilaceaeThiobacillus
ProteomesiUP000008291: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Siroheme synthaseUniRule annotation
PRO_0000330565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi292415.Tbd_2471.

Structurei

3D structure databases

ProteinModelPortaliQ3SG32.
SMRiQ3SG32. Positions 1-459.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 202202precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarity
Add
BLAST
Regioni217 – 479263Uroporphyrinogen-III C-methyltransferase By similarity
Add
BLAST
Regioni302 – 3043S-adenosyl-L-methionine binding By similarity
Regioni332 – 3332S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

In the C-terminal section; belongs to the precorrin methyltransferase family.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiNRVGQAY.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3SG32-1 [UniParc]FASTAAdd to Basket

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MNYLPIFLDL RDRHCLVVGG SETAARKAEL LLRAGAHVAV AAPALHAGFE    50
QLPDRQRLTR VADTFSPALL DGKDAVIVVE DDAAAAQIVA DAARARHLPV 100
NVADKPALCS FILPSIIDRS PIMVAVSSGG ESPVLARLLR ARLETLIPAA 150
YGRLSALASR YKARVREAIQ PGQRRAFWEK VFLSSVAEMV FSGRDSEAEA 200
QLEAMIKDSA AHEPARGEVY LVGAGPGNPD LLTFRALRLM QQADVVVYDR 250
LVSQPILDMC RRDAERIYVG KERDDHAVPQ EEINMMLVRL AKEGKRTLRL 300
KGGDPFIFGR GGEEIETLVE HGVAFQVVPG ITAAAGVASY AGIPLTHRDY 350
AQSVAFVTGH LKENTFNMNW EGIARRDQTI VIYMGLKGLP MLCEALIKHG 400
LTADTPAAIV QHGTLPTQRV ITGTLATLPT LAVEAGLKAP TLIIVGNVVK 450
LREKLAWYRP QAAGEAAAAT PLEAPDHLA 479
Length:479
Mass (Da):51,742
Last modified:October 11, 2005 - v1
Checksum:i657A2AD46D5B5EC9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000116 Genomic DNA. Translation: AAZ98424.1.
RefSeqiYP_316229.1. NC_007404.1.

Genome annotation databases

EnsemblBacteriaiAAZ98424; AAZ98424; Tbd_2471.
GeneIDi3671790.
KEGGitbd:Tbd_2471.
PATRICi23971137. VBIThiDen82923_2455.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000116 Genomic DNA. Translation: AAZ98424.1 .
RefSeqi YP_316229.1. NC_007404.1.

3D structure databases

ProteinModelPortali Q3SG32.
SMRi Q3SG32. Positions 1-459.
ModBasei Search...

Protein-protein interaction databases

STRINGi 292415.Tbd_2471.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ98424 ; AAZ98424 ; Tbd_2471 .
GeneIDi 3671790.
KEGGi tbd:Tbd_2471.
PATRICi 23971137. VBIThiDen82923_2455.

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290518.
KOi K02302.
OMAi NRVGQAY.
OrthoDBi EOG6DRPFR.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci TDEN292415:GHWG-2521-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans."
    Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P.
    J. Bacteriol. 188:1473-1488(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25259.

Entry informationi

Entry nameiCYSG_THIDA
AccessioniPrimary (citable) accession number: Q3SG32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 11, 2005
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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