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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Thiobacillus denitrificans (strain ATCC 25259)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. dCTP deaminase (dcd)
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78Substrate1
Binding sitei92Substrate; via amide nitrogen and carbonyl oxygen1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Name:dut
Ordered Locus Names:Tbd_2586
OrganismiThiobacillus denitrificans (strain ATCC 25259)
Taxonomic identifieri292415 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesThiobacillaceaeThiobacillus
Proteomesi
  • UP000008291 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002314331 – 146Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST146

Interactioni

Protein-protein interaction databases

STRINGi292415.Tbd_2586.

Structurei

3D structure databases

ProteinModelPortaliQ3SFR7.
SMRiQ3SFR7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 67Substrate binding3
Regioni82 – 84Substrate binding3

Sequence similaritiesi

Belongs to the dUTPase family.

Phylogenomic databases

eggNOGiENOG4108Z1K. Bacteria.
COG0756. LUCA.
HOGENOMiHOG000028968.
KOiK01520.
OMAiGVILINH.
OrthoDBiPOG091H01I6.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
HAMAPiMF_00116. dUTPase_bact. 1 hit.
InterProiView protein in InterPro
IPR029054. dUTPase-like.
IPR036157. dUTPase-like_sf.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
PfamiView protein in Pfam
PF00692. dUTPase. 1 hit.
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3SFR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVKILDARL RDQLPHYATP GAAGLDLRAC IDAPITLAPG ETRLVPTGMA
60 70 80 90 100
IHLADPGYAA LILPRSGLGH KHGIVLGNLV GLIDSDYQGQ LMVSAWNRSE
110 120 130 140
QSFELVPLER LAQLVIVPVV QARFNIVEAF ETTARGEGGF GSTGRQ
Length:146
Mass (Da):15,623
Last modified:October 11, 2005 - v1
Checksum:i9203989EDEDC7179
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000116 Genomic DNA. Translation: AAZ98539.1.

Genome annotation databases

EnsemblBacteriaiAAZ98539; AAZ98539; Tbd_2586.
KEGGitbd:Tbd_2586.

Similar proteinsi

Entry informationi

Entry nameiDUT_THIDA
AccessioniPrimary (citable) accession number: Q3SFR7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 11, 2005
Last modified: October 25, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families