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Protein

Autophagy protein 5

Gene

ATG5

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway (By similarity).By similarity
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Autophagy, Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy protein 5
Gene namesi
Name:ATG5
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Autophagy protein 5PRO_0000218993Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Cross-linki130 – 130Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12)By similarity

Post-translational modificationi

Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.By similarity
Acetylated by EP300.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ3MQ24.
PRIDEiQ3MQ24.

Interactioni

Subunit structurei

Forms a conjugate with ATG12. The ATG5-ATG12 conjugate forms a complex with several units of ATG16L. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5-ATG12 conjugate (By similarity). ATG12-ATG5 also interacts with MAVS, MGA, and RARRES3 (By similarity). Interacts with ATG3, ATG7 and ATG10. Interacts with FADD (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007100.

Structurei

3D structure databases

ProteinModelPortaliQ3MQ24.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG5 family.Curated

Phylogenomic databases

eggNOGiKOG2976. Eukaryota.
ENOG410XQ71. LUCA.
HOGENOMiHOG000007893.
HOVERGENiHBG018731.
InParanoidiQ3MQ24.
KOiK08339.

Family and domain databases

InterProiIPR007239. Atg5.
[Graphical view]
PANTHERiPTHR13040. PTHR13040. 1 hit.
PfamiPF04106. APG5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3MQ24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD
60 70 80 90 100
KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI
110 120 130 140 150
TVHFKSFPEK DLLHCPSKDV IEAHFMSCVK EADALKHKSQ VINEMQKKDH
160 170 180 190 200
KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ
210 220 230 240 250
KLFRPVSTDG QLHTLGDLLK EVCPSAVAPE DGEKKNQVMI HGIEPMLETP
260 270
LQWLSEHLSY PDNFLHISII PQPTD
Length:275
Mass (Da):32,431
Last modified:October 3, 2006 - v2
Checksum:iDEF2EE50B38BB34B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti235 – 2351K → N in CAJ31263 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM086994 mRNA. Translation: CAJ31263.1.
BC102770 mRNA. Translation: AAI02771.1.
RefSeqiNP_001029751.2. NM_001034579.2.
UniGeneiBt.27505.

Genome annotation databases

GeneIDi532686.
KEGGibta:532686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM086994 mRNA. Translation: CAJ31263.1.
BC102770 mRNA. Translation: AAI02771.1.
RefSeqiNP_001029751.2. NM_001034579.2.
UniGeneiBt.27505.

3D structure databases

ProteinModelPortaliQ3MQ24.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007100.

Proteomic databases

PaxDbiQ3MQ24.
PRIDEiQ3MQ24.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi532686.
KEGGibta:532686.

Organism-specific databases

CTDi9474.

Phylogenomic databases

eggNOGiKOG2976. Eukaryota.
ENOG410XQ71. LUCA.
HOGENOMiHOG000007893.
HOVERGENiHBG018731.
InParanoidiQ3MQ24.
KOiK08339.

Family and domain databases

InterProiIPR007239. Atg5.
[Graphical view]
PANTHERiPTHR13040. PTHR13040. 1 hit.
PfamiPF04106. APG5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Phylogeny and biochemistry of the autophagy protein beclin 1."
    Botti J., Djavaheri-Mergny M., Codogno P., Oriol R.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiATG5_BOVIN
AccessioniPrimary (citable) accession number: Q3MQ24
Secondary accession number(s): Q3SZN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 3, 2006
Last modified: June 8, 2016
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.