Q3MJK5 (CDK12_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 70.
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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Cyclin-dependent kinase 12 EC=2.7.11.22 EC=2.7.11.23 Alternative name(s): Cdc2-related kinase, arginine/serine-rich Short name=CrkRS Cell division cycle 2-related protein kinase 7 Short name=CDC2-related protein kinase 7 Cell division protein kinase 12 Protein kinase for splicing component | ||||
| Gene names |
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| Organism | Rattus norvegicus (Rat) [Reference proteome] | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 1484 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors By similarity. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Ref.1 |
| Catalytic activity | ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate. ATP + a protein = ADP + a phosphoprotein. |
| Subunit structure | Interacts with CCNL1 and CCNL2. Ref.1 |
| Subcellular location | Nucleus. Nucleus speckle. Note: Colocalized with nuclear speckles throughout interphase. Ref.1 |
| Tissue specificity | Expressed in embryonic tissues such as brain, spinal cord, heart, lung, liver, gut and limb. Levels are lower in adult tissues. Ref.1 |
| Developmental stage | Expressed at E10.5 and E14.5. Ref.1 |
| Sequence similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q3MJK5-1) Also known as: CDK12(L); This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q3MJK5-2) Also known as: CDK12(S); The sequence of this isoform differs from the canonical sequence as follows: 1250-1258: ACPPHILPP → GKQTGHESQ 1259-1484: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1484 | 1484 | Cyclin-dependent kinase 12 | PRO_0000314471 | |||||
Regions | |||||||||
| Domain | 723 – 1016 | 294 | Protein kinase | ||||||
| Nucleotide binding | 729 – 737 | 9 | ATP By similarity | ||||||
| Compositional bias | 137 – 393 | 257 | Ser-rich | ||||||
| Compositional bias | 406 – 412 | 7 | Poly-Ala | ||||||
| Compositional bias | 524 – 703 | 180 | Pro-rich | ||||||
| Compositional bias | 1234 – 1276 | 43 | Pro-rich | ||||||
Sites | |||||||||
| Active site | 855 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 752 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 57 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 73 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 235 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 248 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 273 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 275 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 278 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 300 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 302 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 309 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 311 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 317 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 318 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 322 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 324 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 331 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 332 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 333 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 337 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 344 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 382 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 384 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 399 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 419 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 422 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 511 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 677 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 681 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 688 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 889 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 1049 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1079 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1240 | 1 | Phosphothreonine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 1250 – 1258 | 9 | ACPPHILPP → GKQTGHESQ in isoform 2. | VSP_030288 | |||||
| Alternative sequence | 1259 – 1484 | 226 | Missing in isoform 2. | VSP_030289 | |||||
Sequences
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References
| [1] | "Identification and characterization of the CDK12/cyclin L1 complex involved in alternative splicing regulation." Chen H.-H., Wang Y.-C., Fann M.-J. Mol. Cell. Biol. 26:2736-2745(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH CCNL1 AND CCNL2, SUBCELLULAR LOCATION, FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY. Strain: Sprague-Dawley. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY072294 mRNA. Translation: AAL69525.1. AY962568 mRNA. Translation: AAY41734.1. |
| IPI | IPI00200175. IPI00569900. |
| RefSeq | NP_001029039.1. NM_001033867.1. NP_620271.1. NM_138916.2. |
| UniGene | Rn.48817. |
3D structure databases | |
| HSSP | HSSP built from PDB template 2CJM based on UniProtKB P24941. |
| ProteinModelPortal | Q3MJK5. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 10116.ENSRNOP00000008256. |
PTM databases | |
| PhosphoSite | Q3MJK5. |
Proteomic databases | |
| PaxDb | Q3MJK5. |
| PRIDE | Q3MJK5. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSRNOT00000008256; ENSRNOP00000008256; ENSRNOG00000006000. |
| GeneID | 192350. |
| KEGG | rno:192350. |
| UCSC | RGD:621111. rat. |
Organism-specific databases | |
| CTD | 51755. |
| RGD | 621111. Cdk12. |
Phylogenomic databases | |
| eggNOG | COG0515. |
| GeneTree | ENSGT00690000102025. |
| HOGENOM | HOG000049118. |
| HOVERGEN | HBG050852. |
| InParanoid | Q3MJK5. |
| KO | K08819. |
| OMA | LPHEHQA. |
| OrthoDB | EOG498V0D. |
Gene expression databases | |
| ArrayExpress | Q3MJK5. |
| Genevestigator | Q3MJK5. |
Family and domain databases | |
| InterPro | IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| SUPFAM | SSF56112. Kinase_like. 1 hit. |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 623019. |
Entry information
| Entry name | CDK12_RAT | ||||||||
| Accession | Primary (citable) accession number: Q3MJK5 Secondary accession number(s): Q8R458 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |