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Protein

Putative tRNA pseudouridine synthase Pus10

Gene

PUS10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.Curated2 Publications

Catalytic activityi

tRNA uridine = tRNA pseudouridine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei344NucleophileSequence analysis1
Binding sitei414SubstrateSequence analysis1
Binding sitei485SubstrateSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciZFISH:HS14989-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative tRNA pseudouridine synthase Pus10 (EC:5.4.99.-)
Alternative name(s):
Coiled-coil domain-containing protein 139
tRNA pseudouridine 55 synthase
Short name:
Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase
Gene namesi
Name:PUS10
Synonyms:CCDC139, DOBI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:26505. PUS10.

Pathology & Biotechi

Organism-specific databases

DisGeNETi150962.
OpenTargetsiENSG00000162927.
PharmGKBiPA162400393.

Polymorphism and mutation databases

BioMutaiPUS10.
DMDMi121942830.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002990221 – 529Putative tRNA pseudouridine synthase Pus10Add BLAST529

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79PhosphoserineCombined sources1
Modified residuei84PhosphoserineCombined sources1

Post-translational modificationi

Proteolytically cleaved during TRAIL-induced cell death. Cleaved, in vitro, either by caspase-3 or caspase-8.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3MIT2.
MaxQBiQ3MIT2.
PaxDbiQ3MIT2.
PeptideAtlasiQ3MIT2.
PRIDEiQ3MIT2.

PTM databases

iPTMnetiQ3MIT2.
PhosphoSitePlusiQ3MIT2.

Expressioni

Gene expression databases

BgeeiENSG00000162927.
CleanExiHS_PUS10.
ExpressionAtlasiQ3MIT2. baseline and differential.
GenevisibleiQ3MIT2. HS.

Organism-specific databases

HPAiHPA044736.
HPA049582.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
FAM206AQ9NX384EBI-11983583,EBI-9105722

Protein-protein interaction databases

BioGridi127335. 1 interactor.
IntActiQ3MIT2. 4 interactors.
STRINGi9606.ENSP00000326003.

Structurei

Secondary structure

1529
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Helixi9 – 18Combined sources10
Helixi22 – 28Combined sources7
Helixi35 – 38Combined sources4
Helixi41 – 52Combined sources12
Turni110 – 117Combined sources8
Helixi118 – 120Combined sources3
Helixi122 – 134Combined sources13
Beta strandi142 – 147Combined sources6
Helixi152 – 169Combined sources18
Helixi176 – 178Combined sources3
Helixi182 – 198Combined sources17
Beta strandi207 – 215Combined sources9
Helixi217 – 220Combined sources4
Helixi221 – 227Combined sources7
Helixi246 – 255Combined sources10
Helixi258 – 264Combined sources7
Beta strandi277 – 285Combined sources9
Beta strandi288 – 296Combined sources9
Beta strandi307 – 315Combined sources9
Helixi318 – 323Combined sources6
Helixi326 – 330Combined sources5
Beta strandi333 – 341Combined sources9
Beta strandi348 – 362Combined sources15
Helixi370 – 381Combined sources12
Beta strandi385 – 395Combined sources11
Helixi398 – 409Combined sources12
Beta strandi412 – 422Combined sources11
Helixi426 – 429Combined sources4
Helixi430 – 434Combined sources5
Beta strandi437 – 443Combined sources7
Helixi446 – 448Combined sources3
Turni449 – 451Combined sources3
Beta strandi456 – 470Combined sources15
Beta strandi473 – 480Combined sources8
Helixi486 – 491Combined sources6
Turni493 – 496Combined sources4
Beta strandi497 – 499Combined sources3
Helixi501 – 505Combined sources5
Beta strandi509 – 519Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V9KX-ray2.00A1-529[»]
ProteinModelPortaliQ3MIT2.
SMRiQ3MIT2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3MIT2.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni304 – 317RNA binding forefinger loopSequence analysisAdd BLAST14
Regioni442 – 457RNA binding thumb loopSequence analysisAdd BLAST16

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili42 – 89Sequence analysisAdd BLAST48

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2364. Eukaryota.
COG1258. LUCA.
GeneTreeiENSGT00390000007529.
HOGENOMiHOG000030855.
HOVERGENiHBG056180.
InParanoidiQ3MIT2.
KOiK07583.
OMAiIKKVCQK.
OrthoDBiEOG091G06K1.
PhylomeDBiQ3MIT2.
TreeFamiTF106109.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3MIT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPLTEENKH VAQLLLNTGT CPRCIFRFCG VDFHAPYKLP YKELLNELQK
60 70 80 90 100
FLETEKDELI LEVMNPPPKK IRLQELEDSI DNLSQNGEGR ISVSHVGSTA
110 120 130 140 150
SKNSNLNVCN VCLGILQEFC EKDFIKKVCQ KVEASGFEFT SLVFSVSFPP
160 170 180 190 200
QLSVREHAAW LLVKQEMGKQ SLSLGRDDIV QLKEAYKWIT HPLFSEELGV
210 220 230 240 250
PIDGKSLFEV SVVFAHPETV EDCHFLAAIC PDCFKPAKNK QSVFTRMAVM
260 270 280 290 300
KALNKIKEED FLKQFPCPPN SPKAVCAVLE IECAHGAVFV AGRYNKYSRN
310 320 330 340 350
LPQTPWIIDG ERKLESSVEE LISDHLLAVF KAESFNFSSS GREDVDVRTL
360 370 380 390 400
GNGRPFAIEL VNPHRVHFTS QEIKELQQKI NNSSNKIQVR DLQLVTREAI
410 420 430 440 450
GHMKEGEEEK TKTYSALIWT NKAIQKKDIE FLNDIKDLKI DQKTPLRVLH
460 470 480 490 500
RRPLAVRARV IHFMETQYVD EHHFRLHLKT QAGTYIKEFV HGDFGRTKPN
510 520
IGSLMNVTAD ILELDVESVD VDWPPALDD
Length:529
Mass (Da):60,244
Last modified:October 25, 2005 - v1
Checksum:i44E7BD6ED9C9864E
GO

Sequence cautioni

The sequence CAI46123 differs from that shown. Partially unspliced pre-RNA.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti208F → L in BAB71300 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035617484T → I in a colorectal cancer sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056874 mRNA. Translation: BAB71300.1.
AL832208 Transcribed RNA. Translation: CAI46123.1. Sequence problems.
CH471053 Genomic DNA. Translation: EAX00024.1.
BC101680 mRNA. Translation: AAI01681.1.
BC101706 mRNA. Translation: AAI01707.1.
CCDSiCCDS1865.1.
RefSeqiNP_001309052.1. NM_001322123.1.
NP_001309053.1. NM_001322124.1.
NP_653310.2. NM_144709.3.
XP_011530870.1. XM_011532568.2.
XP_011530872.1. XM_011532570.1.
XP_011530873.1. XM_011532571.1.
XP_011530874.1. XM_011532572.1.
XP_011530875.1. XM_011532573.2.
XP_011530876.1. XM_011532574.2.
XP_011530878.1. XM_011532576.2.
UniGeneiHs.368348.

Genome annotation databases

EnsembliENST00000316752; ENSP00000326003; ENSG00000162927.
ENST00000407787; ENSP00000386074; ENSG00000162927.
GeneIDi150962.
KEGGihsa:150962.
UCSCiuc002sao.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056874 mRNA. Translation: BAB71300.1.
AL832208 Transcribed RNA. Translation: CAI46123.1. Sequence problems.
CH471053 Genomic DNA. Translation: EAX00024.1.
BC101680 mRNA. Translation: AAI01681.1.
BC101706 mRNA. Translation: AAI01707.1.
CCDSiCCDS1865.1.
RefSeqiNP_001309052.1. NM_001322123.1.
NP_001309053.1. NM_001322124.1.
NP_653310.2. NM_144709.3.
XP_011530870.1. XM_011532568.2.
XP_011530872.1. XM_011532570.1.
XP_011530873.1. XM_011532571.1.
XP_011530874.1. XM_011532572.1.
XP_011530875.1. XM_011532573.2.
XP_011530876.1. XM_011532574.2.
XP_011530878.1. XM_011532576.2.
UniGeneiHs.368348.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V9KX-ray2.00A1-529[»]
ProteinModelPortaliQ3MIT2.
SMRiQ3MIT2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127335. 1 interactor.
IntActiQ3MIT2. 4 interactors.
STRINGi9606.ENSP00000326003.

PTM databases

iPTMnetiQ3MIT2.
PhosphoSitePlusiQ3MIT2.

Polymorphism and mutation databases

BioMutaiPUS10.
DMDMi121942830.

Proteomic databases

EPDiQ3MIT2.
MaxQBiQ3MIT2.
PaxDbiQ3MIT2.
PeptideAtlasiQ3MIT2.
PRIDEiQ3MIT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316752; ENSP00000326003; ENSG00000162927.
ENST00000407787; ENSP00000386074; ENSG00000162927.
GeneIDi150962.
KEGGihsa:150962.
UCSCiuc002sao.4. human.

Organism-specific databases

CTDi150962.
DisGeNETi150962.
GeneCardsiPUS10.
HGNCiHGNC:26505. PUS10.
HPAiHPA044736.
HPA049582.
MIMi612787. gene.
neXtProtiNX_Q3MIT2.
OpenTargetsiENSG00000162927.
PharmGKBiPA162400393.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2364. Eukaryota.
COG1258. LUCA.
GeneTreeiENSGT00390000007529.
HOGENOMiHOG000030855.
HOVERGENiHBG056180.
InParanoidiQ3MIT2.
KOiK07583.
OMAiIKKVCQK.
OrthoDBiEOG091G06K1.
PhylomeDBiQ3MIT2.
TreeFamiTF106109.

Enzyme and pathway databases

BioCyciZFISH:HS14989-MONOMER.

Miscellaneous databases

ChiTaRSiPUS10. human.
EvolutionaryTraceiQ3MIT2.
GenomeRNAii150962.
PROiQ3MIT2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162927.
CleanExiHS_PUS10.
ExpressionAtlasiQ3MIT2. baseline and differential.
GenevisibleiQ3MIT2. HS.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPUS10_HUMAN
AccessioniPrimary (citable) accession number: Q3MIT2
Secondary accession number(s): Q5JPJ5, Q96MI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 25, 2005
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.