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Q3MIS6 (ZN528_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein 528
Gene names
Name:ZNF528
Synonyms:KIAA1827
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length628 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be involved in transcriptional regulation.

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 15 C2H2-type zinc fingers.

Contains 1 KRAB domain.

Sequence caution

The sequence BAB47456.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3MIS6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3MIS6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-233: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 628628Zinc finger protein 528
PRO_0000280418

Regions

Domain8 – 7972KRAB
Zinc finger213 – 23523C2H2-type 1
Zinc finger241 – 26323C2H2-type 2
Zinc finger269 – 29123C2H2-type 3
Zinc finger297 – 31923C2H2-type 4
Zinc finger325 – 34723C2H2-type 5
Zinc finger353 – 37523C2H2-type 6
Zinc finger381 – 40323C2H2-type 7
Zinc finger409 – 43123C2H2-type 8
Zinc finger437 – 45923C2H2-type 9
Zinc finger465 – 48723C2H2-type 10
Zinc finger493 – 51523C2H2-type 11
Zinc finger521 – 54323C2H2-type 12
Zinc finger549 – 57123C2H2-type 13
Zinc finger577 – 59923C2H2-type 14
Zinc finger605 – 62723C2H2-type 15

Natural variations

Alternative sequence1 – 233233Missing in isoform 2.
VSP_023662
Natural variant4191S → N.
Corresponds to variant rs324109 [ dbSNP | Ensembl ].
VAR_052858

Experimental info

Sequence conflict2971Y → H in CAD89949. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 25, 2005. Version 1.
Checksum: BB8704D4A354ED11

FASTA62872,138
        10         20         30         40         50         60 
MALTQGPLKF MDVAIEFSQE EWKCLDPAQR TLYRDVMLEN YRNLVSLGIC LPDLSVTSML 

        70         80         90        100        110        120 
EQKRDPWTLQ SEEKIANDPD GRECIKGVNT ERSSKLGSNA GNKPCKNQLG FTFQLHLSDL 

       130        140        150        160        170        180 
QLFQAERKIS GCKHFEKPVS DNSSVSPLEK ISSSVKSHLL NKYRNNFDHA PLLPQEQKAH 

       190        200        210        220        230        240 
IREKAYKCNE HGQVFRASAS LTNQVIHNAD NPYKCSECGK VFSCSSKLVI HRRMHTGEKP 

       250        260        270        280        290        300 
YKCHECGKLF SSNSNLSQHQ RIHTGEKPYK CHECDKVFRS SSKLAQHQRI HTGEKPYKCH 

       310        320        330        340        350        360 
ECDKVFNQIA HLVRHQKIHT GEKPYSCNKC GKVFSRHSYL AEHQTVHTGE KPYKCEECGK 

       370        380        390        400        410        420 
AFSVRSSLIT HQLIHTGRKP YKCKECDKVF GRKCFLTSHQ RIHTRERPYG CSQCGKIFSQ 

       430        440        450        460        470        480 
KSDLIRHRKT HTDEKPYKCN KCGTAFREFS DLTAHFLIHS GEKPYECKEC GKVFRYKSSL 

       490        500        510        520        530        540 
TSHHRIHTGE KPYKCNRCGK VFSRSSNLVC HQKIHTGEKP YKCNQCGKVF NQASYLTRHQ 

       550        560        570        580        590        600 
IIHTGERPYR CSKCGKAFRG CSGLTAHLAI HTEKKSHECK ECGKIFTQKS SLTNHHRIHI 

       610        620 
GEKPYKCTLC SKVFSHNSDL AQHQRVHS 

« Hide

Isoform 2 [UniParc].

Checksum: 04D697F0F7C21732
Show »

FASTA39545,715

References

[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Spinal cord.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Heart and Lung.
[5]"Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.
DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 166-628 (ISOFORM 1).
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK056552 mRNA. Translation: BAG51746.1.
AL832630 mRNA. Translation: CAD89949.1.
CH471135 Genomic DNA. Translation: EAW72078.1.
BC101712 mRNA. Translation: AAI01713.1.
BC111950 mRNA. Translation: AAI11951.1.
AB058730 mRNA. Translation: BAB47456.1. Different initiation.
CCDSCCDS33091.1. [Q3MIS6-1]
RefSeqNP_115799.2. NM_032423.2. [Q3MIS6-1]
XP_006723482.1. XM_006723419.1. [Q3MIS6-2]
UniGeneHs.662043.

3D structure databases

ProteinModelPortalQ3MIS6.
SMRQ3MIS6. Positions 128-628.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124076. 2 interactions.

PTM databases

PhosphoSiteQ3MIS6.

Polymorphism databases

DMDM121942828.

Proteomic databases

PaxDbQ3MIS6.
PRIDEQ3MIS6.

Protocols and materials databases

DNASU84436.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000360465; ENSP00000353652; ENSG00000167555. [Q3MIS6-1]
GeneID84436.
KEGGhsa:84436.
UCSCuc002pzh.3. human. [Q3MIS6-1]

Organism-specific databases

CTD84436.
GeneCardsGC19P052901.
HGNCHGNC:29384. ZNF528.
MIM615580. gene.
neXtProtNX_Q3MIS6.
PharmGKBPA134869369.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000234617.
HOVERGENHBG018163.
KOK09228.
OMACHECDKV.
OrthoDBEOG7KSX7Q.
PhylomeDBQ3MIS6.
TreeFamTF341892.

Enzyme and pathway databases

ReactomeREACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ3MIS6.
BgeeQ3MIS6.
CleanExHS_ZNF528.
GenevestigatorQ3MIS6.

Family and domain databases

Gene3D3.30.160.60. 15 hits.
InterProIPR001909. Krueppel-associated_box.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF01352. KRAB. 1 hit.
[Graphical view]
SMARTSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 15 hits.
[Graphical view]
SUPFAMSSF109640. SSF109640. 1 hit.
PROSITEPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 15 hits.
PS50157. ZINC_FINGER_C2H2_2. 15 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi84436.
NextBio74179.
PROQ3MIS6.
SOURCESearch...

Entry information

Entry nameZN528_HUMAN
AccessionPrimary (citable) accession number: Q3MIS6
Secondary accession number(s): B3KPN4, Q86T88, Q96JK0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 25, 2005
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM