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Protein

Cell cycle control protein 50B

Gene

TMEM30B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accessory component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. The beta subunit may assist in binding of the phospholipid substrate (Probable). Can mediate the export of alpha subunits ATP8A1, ATP8B1, ATP8B2 and ATP8B4 from the ER to the plasma membrane.1 PublicationCurated

GO - Biological processi

  1. lipid transport Source: UniProtKB-KW
  2. positive regulation of protein exit from endoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Protein family/group databases

TCDBi8.A.27.1.6. the cdc50 p-type atpase lipid flippase subunit (cdc50) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell cycle control protein 50B
Alternative name(s):
P4-ATPase flippase complex beta subunit TMEM30B
Transmembrane protein 30B
Gene namesi
Name:TMEM30B
Synonyms:CDC50B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:27254. TMEM30B.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei34 – 5421HelicalSequence AnalysisAdd
BLAST
Topological domaini55 – 315261Exoplasmic loopSequence AnalysisAdd
BLAST
Transmembranei316 – 33621HelicalSequence AnalysisAdd
BLAST
Topological domaini337 – 35115CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134993260.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Cell cycle control protein 50BPRO_0000244474Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ3MIR4.
PaxDbiQ3MIR4.
PRIDEiQ3MIR4.

Expressioni

Gene expression databases

BgeeiQ3MIR4.
CleanExiHS_TMEM30B.
GenevestigatoriQ3MIR4.

Organism-specific databases

HPAiHPA043162.

Interactioni

Subunit structurei

Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit (Probable). Interacts with alpha subunits ATP8A1, ATP8B1, ATP8B2 and ATP8B4.3 PublicationsCurated

Protein-protein interaction databases

BioGridi127780. 1 interaction.
IntActiQ3MIR4. 3 interactions.
MINTiMINT-5004611.
STRINGi9606.ENSP00000347930.

Structurei

3D structure databases

ProteinModelPortaliQ3MIR4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi344 – 3496Poly-Asp

Sequence similaritiesi

Belongs to the CDC50/LEM3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5035.
GeneTreeiENSGT00390000004660.
HOGENOMiHOG000209057.
HOVERGENiHBG055537.
InParanoidiQ3MIR4.
OMAiPVNECAP.
OrthoDBiEOG7C5M8D.
PhylomeDBiQ3MIR4.
TreeFamiTF300873.

Family and domain databases

InterProiIPR005045. CDC50/LEM3_fam.
IPR030352. TMEM30B.
[Graphical view]
PANTHERiPTHR10926. PTHR10926. 1 hit.
PTHR10926:SF19. PTHR10926:SF19. 1 hit.
PfamiPF03381. CDC50. 1 hit.
[Graphical view]
PIRSFiPIRSF015840. DUF284_TM_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3MIR4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTWSATARGA HQPDNTAFTQ QRLPAWQPLL SASIALPLFF CAGLAFIGLG
60 70 80 90 100
LGLYYSSNGI KELEYDYTGD PGTGNCSVCA AAGQGRALPP PCSCAWYFSL
110 120 130 140 150
PELFQGPVYL YYELTNFYQN NRRYGVSRDD AQLSGLPSAL RHPVNECAPY
160 170 180 190 200
QRSAAGLPIA PCGAIANSLF NDSFSLWHQR QPGGPYVEVP LDRSGIAWWT
210 220 230 240 250
DYHVKFRNPP LVNGSLALAF QGTAPPPNWR RPVYELSPDP NNTGFINQDF
260 270 280 290 300
VVWMRTAALP TFRKLYARIR QGNYSAGLPR GAYRVNITYN YPVRAFGGHK
310 320 330 340 350
LLIFSSISWM GGKNPFLGIA YLVVGSLCIL TGFVMLVVYI RYQDQDDDDE

E
Length:351
Mass (Da):38,941
Last modified:October 25, 2005 - v1
Checksum:iB68C91A784E33A44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK091169 mRNA. Translation: BAG52296.1.
CH471061 Genomic DNA. Translation: EAW80799.1.
BC101726 mRNA. Translation: AAI01727.1.
BC113559 mRNA. Translation: AAI13560.1.
CCDSiCCDS32093.1.
RefSeqiNP_001017970.1. NM_001017970.2.
UniGeneiHs.146180.

Genome annotation databases

EnsembliENST00000555868; ENSP00000450842; ENSG00000182107.
GeneIDi161291.
KEGGihsa:161291.
UCSCiuc001xfl.3. human.

Polymorphism databases

DMDMi109819759.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK091169 mRNA. Translation: BAG52296.1.
CH471061 Genomic DNA. Translation: EAW80799.1.
BC101726 mRNA. Translation: AAI01727.1.
BC113559 mRNA. Translation: AAI13560.1.
CCDSiCCDS32093.1.
RefSeqiNP_001017970.1. NM_001017970.2.
UniGeneiHs.146180.

3D structure databases

ProteinModelPortaliQ3MIR4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127780. 1 interaction.
IntActiQ3MIR4. 3 interactions.
MINTiMINT-5004611.
STRINGi9606.ENSP00000347930.

Protein family/group databases

TCDBi8.A.27.1.6. the cdc50 p-type atpase lipid flippase subunit (cdc50) family.

Polymorphism databases

DMDMi109819759.

Proteomic databases

MaxQBiQ3MIR4.
PaxDbiQ3MIR4.
PRIDEiQ3MIR4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000555868; ENSP00000450842; ENSG00000182107.
GeneIDi161291.
KEGGihsa:161291.
UCSCiuc001xfl.3. human.

Organism-specific databases

CTDi161291.
GeneCardsiGC14M061744.
HGNCiHGNC:27254. TMEM30B.
HPAiHPA043162.
MIMi611029. gene.
neXtProtiNX_Q3MIR4.
PharmGKBiPA134993260.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5035.
GeneTreeiENSGT00390000004660.
HOGENOMiHOG000209057.
HOVERGENiHBG055537.
InParanoidiQ3MIR4.
OMAiPVNECAP.
OrthoDBiEOG7C5M8D.
PhylomeDBiQ3MIR4.
TreeFamiTF300873.

Miscellaneous databases

GenomeRNAii161291.
NextBioi88046.
PROiQ3MIR4.
SOURCEiSearch...

Gene expression databases

BgeeiQ3MIR4.
CleanExiHS_TMEM30B.
GenevestigatoriQ3MIR4.

Family and domain databases

InterProiIPR005045. CDC50/LEM3_fam.
IPR030352. TMEM30B.
[Graphical view]
PANTHERiPTHR10926. PTHR10926. 1 hit.
PTHR10926:SF19. PTHR10926:SF19. 1 hit.
PfamiPF03381. CDC50. 1 hit.
[Graphical view]
PIRSFiPIRSF015840. DUF284_TM_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Tongue.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart and Lung.
  4. "Identification and characterization of CDC50A, CDC50B and CDC50C genes in silico."
    Katoh Y., Katoh M.
    Oncol. Rep. 12:939-943(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "Heteromeric interactions required for abundance and subcellular localization of human CDC50 proteins and class 1 P4-ATPases."
    van der Velden L.M., Wichers C.G., van Breevoort A.E., Coleman J.A., Molday R.S., Berger R., Klomp L.W., van de Graaf S.F.
    J. Biol. Chem. 285:40088-40096(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATP8B1 AND ATP8B2.
  6. "CDC50 proteins are critical components of the human class-1 P4-ATPase transport machinery."
    Bryde S., Hennrich H., Verhulst P.M., Devaux P.F., Lenoir G., Holthuis J.C.
    J. Biol. Chem. 285:40562-40572(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATP8A1; ATP8B1; ATP8B2 AND ATP8B4.
  7. "ATP9B, a P4-ATPase (a putative aminophospholipid translocase), localizes to the trans-Golgi network in a CDC50 protein-independent manner."
    Takatsu H., Baba K., Shima T., Umino H., Kato U., Umeda M., Nakayama K., Shin H.W.
    J. Biol. Chem. 286:38159-38167(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATP8B1.

Entry informationi

Entry nameiCC50B_HUMAN
AccessioniPrimary (citable) accession number: Q3MIR4
Secondary accession number(s): B3KR84, Q14D00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: October 25, 2005
Last modified: January 7, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.