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Protein

Ral guanine nucleotide dissociation stimulator-like 3

Gene

RGL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) for Ral-A. Potential effector of GTPase HRas and Ras-related protein M-Ras. Negatively regulates Elk-1-dependent gene induction downstream of HRas and MEKK1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Ral guanine nucleotide dissociation stimulator-like 3
Short name:
RalGDS-like 3
Gene namesi
Name:RGL3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:30282. RGL3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134979213.

Polymorphism and mutation databases

BioMutaiRGL3.
DMDMi296452877.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 710710Ral guanine nucleotide dissociation stimulator-like 3PRO_0000306799Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521PhosphoserineCombined sources
Modified residuei508 – 5081PhosphoserineBy similarity
Modified residuei512 – 5121PhosphoserineCombined sources
Modified residuei555 – 5551PhosphoserineBy similarity
Modified residuei569 – 5691PhosphoserineCombined sources
Modified residuei573 – 5731PhosphoserineCombined sources
Modified residuei591 – 5911PhosphoserineCombined sources
Modified residuei601 – 6011PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3MIN7.
MaxQBiQ3MIN7.
PaxDbiQ3MIN7.
PeptideAtlasiQ3MIN7.
PRIDEiQ3MIN7.

PTM databases

iPTMnetiQ3MIN7.
PhosphoSiteiQ3MIN7.

Expressioni

Gene expression databases

BgeeiQ3MIN7.
CleanExiHS_RGL3.
ExpressionAtlasiQ3MIN7. baseline and differential.
GenevisibleiQ3MIN7. HS.

Organism-specific databases

HPAiHPA042763.
HPA043615.

Interactioni

Subunit structurei

Interacts with GTP-bound forms of RIT1, HRAS and MRAS.By similarity

Protein-protein interaction databases

IntActiQ3MIN7. 1 interaction.
STRINGi9606.ENSP00000377075.

Structurei

3D structure databases

ProteinModelPortaliQ3MIN7.
SMRiQ3MIN7. Positions 12-506, 616-698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 200136N-terminal Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini247 – 505259Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini613 – 70088Ras-associatingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni612 – 70796Interaction with HRAS, MRAS and RIT1By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi231 – 2344Poly-Glu
Compositional biasi537 – 60771Pro-richAdd
BLAST

Domaini

The Ras-associating domain plays a central role in the activation of Ral-A GDP/GTP exchange activity.By similarity

Sequence similaritiesi

Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3629. Eukaryota.
ENOG410ZUT5. LUCA.
HOGENOMiHOG000231592.
HOVERGENiHBG005864.
InParanoidiQ3MIN7.
KOiK17639.
OrthoDBiEOG76DTRS.
PhylomeDBiQ3MIN7.
TreeFamiTF315204.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000159. RA_dom.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR030750. RGL3.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR23113:SF220. PTHR23113:SF220. 2 hits.
PfamiPF00788. RA. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3MIN7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERTAGKELA LAPLQDWGEE TEDGAVYSVS LRRQRSQRRS PAEGPGGSQA
60 70 80 90 100
PSPIANTFLH YRTSKVRVLR AARLERLVGE LVFGDREQDP SFMPAFLATY
110 120 130 140 150
RTFVPTACLL GFLLPPMPPP PPPGVEIKKT AVQDLSFNKN LRAVVSVLGS
160 170 180 190 200
WLQDHPQDFR DPPAHSDLGS VRTFLGWAAP GSAEAQKAEK LLEDFLEEAE
210 220 230 240 250
REQEEEPPQV WTGPPRVAQT SDPDSSEACA EEEEGLMPQG PQLLDFSVDE
260 270 280 290 300
VAEQLTLIDL ELFSKVRLYE CLGSVWSQRD RPGAAGASPT VRATVAQFNT
310 320 330 340 350
VTGCVLGSVL GAPGLAAPQR AQRLEKWIRI AQRCRELRNF SSLRAILSAL
360 370 380 390 400
QSNPIYRLKR SWGAVSREPL STFRKLSQIF SDENNHLSSR EILFQEEATE
410 420 430 440 450
GSQEEDNTPG SLPSKPPPGP VPYLGTFLTD LVMLDTALPD MLEGDLINFE
460 470 480 490 500
KRRKEWEILA RIQQLQRRCQ SYTLSPHPPI LAALHAQNQL TEEQSYRLSR
510 520 530 540 550
VIEPPAASCP SSPRIRRRIS LTKRLSAKLA REKSSSPSGS PGDPSSPTSS
560 570 580 590 600
VSPGSPPSSP RSRDAPAGSP PASPGPQGPS TKLPLSLDLP SPRPFALPLG
610 620 630 640 650
SPRIPLPAQQ SSEARVIRVS IDNDHGNLYR SILLTSQDKA PSVVRRALQK
660 670 680 690 700
HNVPQPWACD YQLFQVLPGD RVLLIPDNAN VFYAMSPVAP RDFMLRRKEG
710
TRNTLSVSPS
Length:710
Mass (Da):78,079
Last modified:May 18, 2010 - v2
Checksum:iE36E9E66201D7F47
GO
Isoform 2 (identifier: Q3MIN7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     549-549: S → SSLCISP

Note: No experimental confirmation available.
Show »
Length:716
Mass (Da):78,680
Checksum:i82B4174DDE0AF933
GO

Sequence cautioni

The sequence AAH14426.1 differs from that shown. Reason: Frameshift at position 323. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti162 – 1621P → H.1 Publication
Corresponds to variant rs167479 [ dbSNP | Ensembl ].
VAR_035298
Natural varianti164 – 1641A → V.1 Publication
Corresponds to variant rs160838 [ dbSNP | Ensembl ].
VAR_035299
Natural varianti615 – 6151R → C.
Corresponds to variant rs2291516 [ dbSNP | Ensembl ].
VAR_035300

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei549 – 5491S → SSLCISP in isoform 2. 1 PublicationVSP_045369

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC024575 Genomic DNA. No translation available.
BC014426 mRNA. Translation: AAH14426.1. Frameshift.
BC101756 mRNA. Translation: AAI01757.1.
BC111958 mRNA. Translation: AAI11959.1.
BC143530 mRNA. Translation: AAI43531.1.
CCDSiCCDS32910.1. [Q3MIN7-1]
CCDS54221.1. [Q3MIN7-2]
RefSeqiNP_001030300.3. NM_001035223.3.
NP_001155088.2. NM_001161616.2.
UniGeneiHs.375142.

Genome annotation databases

EnsembliENST00000393423; ENSP00000377075; ENSG00000205517.
GeneIDi57139.
KEGGihsa:57139.
UCSCiuc002mro.3. human. [Q3MIN7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC024575 Genomic DNA. No translation available.
BC014426 mRNA. Translation: AAH14426.1. Frameshift.
BC101756 mRNA. Translation: AAI01757.1.
BC111958 mRNA. Translation: AAI11959.1.
BC143530 mRNA. Translation: AAI43531.1.
CCDSiCCDS32910.1. [Q3MIN7-1]
CCDS54221.1. [Q3MIN7-2]
RefSeqiNP_001030300.3. NM_001035223.3.
NP_001155088.2. NM_001161616.2.
UniGeneiHs.375142.

3D structure databases

ProteinModelPortaliQ3MIN7.
SMRiQ3MIN7. Positions 12-506, 616-698.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3MIN7. 1 interaction.
STRINGi9606.ENSP00000377075.

PTM databases

iPTMnetiQ3MIN7.
PhosphoSiteiQ3MIN7.

Polymorphism and mutation databases

BioMutaiRGL3.
DMDMi296452877.

Proteomic databases

EPDiQ3MIN7.
MaxQBiQ3MIN7.
PaxDbiQ3MIN7.
PeptideAtlasiQ3MIN7.
PRIDEiQ3MIN7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000393423; ENSP00000377075; ENSG00000205517.
GeneIDi57139.
KEGGihsa:57139.
UCSCiuc002mro.3. human. [Q3MIN7-1]

Organism-specific databases

CTDi57139.
GeneCardsiRGL3.
H-InvDBHIX0080023.
HIX0137471.
HGNCiHGNC:30282. RGL3.
HPAiHPA042763.
HPA043615.
MIMi616743. gene.
neXtProtiNX_Q3MIN7.
PharmGKBiPA134979213.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3629. Eukaryota.
ENOG410ZUT5. LUCA.
HOGENOMiHOG000231592.
HOVERGENiHBG005864.
InParanoidiQ3MIN7.
KOiK17639.
OrthoDBiEOG76DTRS.
PhylomeDBiQ3MIN7.
TreeFamiTF315204.

Miscellaneous databases

ChiTaRSiRGL3. human.
GenomeRNAii57139.
PROiQ3MIN7.
SOURCEiSearch...

Gene expression databases

BgeeiQ3MIN7.
CleanExiHS_RGL3.
ExpressionAtlasiQ3MIN7. baseline and differential.
GenevisibleiQ3MIN7. HS.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000159. RA_dom.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR030750. RGL3.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR23113:SF220. PTHR23113:SF220. 2 hits.
PfamiPF00788. RA. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS HIS-162 AND VAL-164.
    Tissue: Heart, Kidney and Lung.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52 AND SER-512, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  4. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-591 AND SER-601, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512; SER-569 AND SER-573, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-569, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiRGL3_HUMAN
AccessioniPrimary (citable) accession number: Q3MIN7
Secondary accession number(s): B5ME84, B7ZL22, Q0P6G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: May 18, 2010
Last modified: July 6, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.