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Protein

TBC1 domain family member 25

Gene

TBC1D25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a GTPase-activating protein specific for RAB33B. Involved in the regulation of autophagosome maturation, the process in which autophagosomes fuse with endosomes and lysosomes.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB
  • Rab GTPase binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
Biological processAutophagy

Enzyme and pathway databases

ReactomeiR-HSA-8854214 TBC/RABGAPs
SIGNORiQ3MII6

Names & Taxonomyi

Protein namesi
Recommended name:
TBC1 domain family member 25
Gene namesi
Name:TBC1D25
Synonyms:OATL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000068354.15
HGNCiHGNC:8092 TBC1D25
MIMi311240 gene
neXtProtiNX_Q3MII6

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi134 – 135ED → AA: Severely affects interaction with GABARAP. Does not localize at autophagosomes. 1 Publication2
Mutagenesisi136W → A: Abolishes interaction with GABARAP. Does not localize at autophagosomes. 1 Publication1

Organism-specific databases

DisGeNETi4943
OpenTargetsiENSG00000068354
PharmGKBiPA162405201

Polymorphism and mutation databases

BioMutaiTBC1D25
DMDMi296452922

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002885081 – 688TBC1 domain family member 25Add BLAST688

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei140PhosphoserineBy similarity1
Modified residuei160PhosphothreonineBy similarity1
Modified residuei506PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3MII6
MaxQBiQ3MII6
PaxDbiQ3MII6
PeptideAtlasiQ3MII6
PRIDEiQ3MII6

PTM databases

iPTMnetiQ3MII6
PhosphoSitePlusiQ3MII6

Expressioni

Gene expression databases

BgeeiENSG00000068354
CleanExiHS_TBC1D25
ExpressionAtlasiQ3MII6 baseline and differential
GenevisibleiQ3MII6 HS

Organism-specific databases

HPAiHPA029197

Interactioni

Subunit structurei

Interacts (via N-terminus) with MAP1LC3B, GABARAP and GABARAPL2.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110997, 42 interactors
IntActiQ3MII6, 43 interactors
STRINGi9606.ENSP00000365962

Structurei

3D structure databases

ProteinModelPortaliQ3MII6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini228 – 434Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST207

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 28Poly-Ala7
Compositional biasi29 – 34Poly-Glu6
Compositional biasi494 – 499Poly-Gly6
Compositional biasi559 – 564Poly-Ser6

Phylogenomic databases

eggNOGiKOG2197 Eukaryota
COG5210 LUCA
GeneTreeiENSGT00550000074386
HOVERGENiHBG103222
InParanoidiQ3MII6
OMAiEHRDHIM
OrthoDBiEOG091G036C
PhylomeDBiQ3MII6
TreeFamiTF323518

Family and domain databases

InterProiView protein in InterPro
IPR000195 Rab-GTPase-TBC_dom
IPR035969 Rab-GTPase_TBC_sf
PfamiView protein in Pfam
PF00566 RabGAP-TBC, 1 hit
SMARTiView protein in SMART
SM00164 TBC, 1 hit
SUPFAMiSSF47923 SSF47923, 3 hits
PROSITEiView protein in PROSITE
PS50086 TBC_RABGAP, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3MII6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATASGASDL SGSGAPPPGV GAQAAAAAEE EEREVVRVRV KKCESFLPPE
60 70 80 90 100
FRSFAVDPQI TSLDVLQHIL IRAFDLSGKK NFGISYLGRD RLGQEVYLSL
110 120 130 140 150
LSDWDLSTAF ATASKPYLQL RVDIRPSEDS PLLEDWDIIS PKDVIGSDVL
160 170 180 190 200
LAEKRSSLTT AALPFTQSIL TQVGRTLSKV QQVLSWSYGE DVKPFKPPLS
210 220 230 240 250
DAEFHTYLNH EGQLSRPEEL RLRIYHGGVE PSLRKVVWRY LLNVYPDGLT
260 270 280 290 300
GRERMDYMKR KSREYEQLKS EWAQRANPED LEFIRSTVLK DVLRTDRAHP
310 320 330 340 350
YYAGPEDGPH LRALHDLLTT YAVTHPQVSY CQGMSDLASP ILAVMDHEGH
360 370 380 390 400
AFVCFCGIMK RLAANFHPDG RAMATKFAHL KLLLRHADPD FYQYLQEAGA
410 420 430 440 450
DDLFFCYRWL LLELKREFAF DDALRMLEVT WSSLPPDPPE HEVELVGPPS
460 470 480 490 500
QVADAGFGGH RGWPVRQRHM LRPAGGGGST FEDAVDHLAT ASQGPGGGGR
510 520 530 540 550
LLRQASLDGL QQLRDNMGSR RDPLVQLPHP AALISSKSLS EPLLNSPDPL
560 570 580 590 600
LSSFSHPDSP SSSSPPSTQE ASPTGDMAVG SPLMQEVGSP KDPGKSLPPV
610 620 630 640 650
PPMGLPPPQE FGRGNPFMLF LCLAILLEHR DHIMRNGLDY NELAMHFDRL
660 670 680
VRKHHLGRVL RRARALFADY LQSEVWDSEE GAEATAAS
Length:688
Mass (Da):76,327
Last modified:May 18, 2010 - v2
Checksum:i5B070FE11D78D0A4
GO
Isoform 2 (identifier: Q3MII6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     79-100: KKNFGISYLGRDRLGQEVYLSL → PIARRLGHNQPQRCHWLRRVAG
     101-688: Missing.

Note: No experimental confirmation available.
Show »
Length:100
Mass (Da):10,757
Checksum:i552B53E4E93159B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti455A → T in AAI01818 (PubMed:15489334).Curated1
Sequence conflicti455A → T in AAI01820 (PubMed:15489334).Curated1
Sequence conflicti455A → T in AAI25089 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057345277N → S1 PublicationCorresponds to variant dbSNP:rs2293948Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02570379 – 100KKNFG…VYLSL → PIARRLGHNQPQRCHWLRRV AG in isoform 2. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_025704101 – 688Missing in isoform 2. 1 PublicationAdd BLAST588

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC115618 Genomic DNA No translation available.
AF196969 Genomic DNA No translation available.
BC026050 mRNA Translation: AAH26050.1
BC101817 mRNA Translation: AAI01818.1
BC101819 mRNA Translation: AAI01820.1
BC125088 mRNA Translation: AAI25089.1
BC125089 mRNA Translation: AAI25090.2
CCDSiCCDS35242.1 [Q3MII6-1]
RefSeqiNP_001335191.1, NM_001348262.1
NP_002527.1, NM_002536.3 [Q3MII6-1]
UniGeneiHs.694353

Genome annotation databases

EnsembliENST00000376771; ENSP00000365962; ENSG00000068354 [Q3MII6-1]
ENST00000481090; ENSP00000476787; ENSG00000068354 [Q3MII6-2]
GeneIDi4943
KEGGihsa:4943
UCSCiuc004dka.2 human [Q3MII6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTBC25_HUMAN
AccessioniPrimary (citable) accession number: Q3MII6
Secondary accession number(s): Q08AN9, Q3MII4, Q8TAR9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 18, 2010
Last modified: April 25, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health