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Protein

Leukotriene-B(4) omega-hydroxylase 2

Gene

Cyp4f3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes (By similarity).By similarity

Catalytic activityi

(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O.By similarity

Cofactori

hemeBy similarity

Enzyme regulationi

Inhibited by carbon monoxide (CO).By similarity

Pathwayi: leukotriene B4 degradation

This protein is involved in the pathway leukotriene B4 degradation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway leukotriene B4 degradation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi468Iron (heme axial ligand)By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

SABIO-RKQ3MID2.
UniPathwayiUPA00883.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukotriene-B(4) omega-hydroxylase 2 (EC:1.14.13.30)
Alternative name(s):
CYPIVF3
Cytochrome P450 4F3
Cytochrome P450-LTB-omega
Leukotriene-B(4) 20-monooxygenase 2
Gene namesi
Name:Cyp4f3By similarity
Synonyms:Cyp4f18Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1305261. Cyp4f18.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002389251 – 524Leukotriene-B(4) omega-hydroxylase 2Add BLAST524

Proteomic databases

PaxDbiQ3MID2.
PRIDEiQ3MID2.

PTM databases

iPTMnetiQ3MID2.
PhosphoSitePlusiQ3MID2.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021609.

Structurei

3D structure databases

ProteinModelPortaliQ3MID2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Sequence analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiQ3MID2.
KOiK17726.
PhylomeDBiQ3MID2.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3MID2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLSLSWLG LGHTAASPWL LLLLVGASCL LAYILPQVYA VFENSRRLRR
60 70 80 90 100
FPQPPPRNWL FGHLGLIQSS EEGLLYIQSL SRTFRDVCCW WVGPWHPVIR
110 120 130 140 150
IFHPAFIKPV ILAPASVAPK DRVFYRFLRP WLGDGLLLST GDKWSRHRRM
160 170 180 190 200
LTPAFHFNIL KPYVKIFNDS TNIMHAKWQR LASQGSARLD MFEHISLMTL
210 220 230 240 250
DSLQKCVFSF DSNCQEKPSE YITAILELSA LVARRHQSLL LHVDLFYHLT
260 270 280 290 300
RDGMRFRKAC RLVHDFTDAV IRERRCTLPD QGGDDALKAK AKAKTLDFID
310 320 330 340 350
VLLLSKDEHG EALSDEDIRA EADTFMFGGH DTTASGLSWI LYNLAKHPEY
360 370 380 390 400
QERCRQEVRE LLRDREPEEI EWDDLAQLPF LTMCIKESLR LHPPATAISR
410 420 430 440 450
CCTQDIMLPD GRVIPKGVIC RISIFGTHHN PAVWPDPEVY NPFRFDADNG
460 470 480 490 500
KGRSPLAFIP FSAGPRNCIG QTFAMSEMKV ALALTLLRFR VLPDDKEPRR
510 520
KPELILRAEG GLWLRVEPLS AGAH
Length:524
Mass (Da):59,906
Last modified:October 25, 2005 - v1
Checksum:i8DC9033E01F1A1E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC101918 mRNA. Translation: AAI01919.1.
RefSeqiNP_001028858.1. NM_001033686.1.
UniGeneiRn.215683.

Genome annotation databases

GeneIDi290623.
KEGGirno:290623.
UCSCiRGD:1305261. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC101918 mRNA. Translation: AAI01919.1.
RefSeqiNP_001028858.1. NM_001033686.1.
UniGeneiRn.215683.

3D structure databases

ProteinModelPortaliQ3MID2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021609.

PTM databases

iPTMnetiQ3MID2.
PhosphoSitePlusiQ3MID2.

Proteomic databases

PaxDbiQ3MID2.
PRIDEiQ3MID2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi290623.
KEGGirno:290623.
UCSCiRGD:1305261. rat.

Organism-specific databases

CTDi72054.
RGDi1305261. Cyp4f18.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiQ3MID2.
KOiK17726.
PhylomeDBiQ3MID2.

Enzyme and pathway databases

UniPathwayiUPA00883.
SABIO-RKQ3MID2.

Miscellaneous databases

PROiQ3MID2.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP4F3_RAT
AccessioniPrimary (citable) accession number: Q3MID2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: October 25, 2005
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.