Reviewed, UniProtKB/Swiss-Prot Q3MID2 (CP4F3_RAT)

Names and origin

Protein names

Recommended name:
    Leukotriene-B(4) omega-hydroxylase 2
    EC=1.14.13.30
Alternative name(s):
    Cytochrome P450 4F3
    CYPIVF3
    Leukotriene-B(4) 20-monooxygenase 2
    Cytochrome P450-LTB-omega

Gene names

Name:	Cyp4f3
Synonyms:	Cyp4f18

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes By similarity. UniProtKB Q99N16 UniProtKB Q08477
Catalytic activity	(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O. UniProtKB Q08477
Cofactor	Heme group By similarity.
Enzyme regulation	Inhibited by carbon monoxide (CO) By similarity. UniProtKB Q08477
Pathway	Lipid metabolism; leukotriene B4 degradation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Microsome membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro leukotriene-B4 20-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Leukotriene-B(4) omega-hydroxylase 2		PRO_0000238925
Regions
	Transmembrane	15 – 35	21	Potential
Sites
	Metal binding	468	1	Iron (heme axial ligand) By similarity UniProtKB P33274

Sequences

	Sequence		Length	Mass (Da)
	Q3MID2-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 8DC9033E01F1A1E8	FASTA	524	59,906
	10 20 30 40 50 60 MPLLSLSWLG LGHTAASPWL LLLLVGASCL LAYILPQVYA VFENSRRLRR FPQPPPRNWL 70 80 90 100 110 120 FGHLGLIQSS EEGLLYIQSL SRTFRDVCCW WVGPWHPVIR IFHPAFIKPV ILAPASVAPK 130 140 150 160 170 180 DRVFYRFLRP WLGDGLLLST GDKWSRHRRM LTPAFHFNIL KPYVKIFNDS TNIMHAKWQR 190 200 210 220 230 240 LASQGSARLD MFEHISLMTL DSLQKCVFSF DSNCQEKPSE YITAILELSA LVARRHQSLL 250 260 270 280 290 300 LHVDLFYHLT RDGMRFRKAC RLVHDFTDAV IRERRCTLPD QGGDDALKAK AKAKTLDFID 310 320 330 340 350 360 VLLLSKDEHG EALSDEDIRA EADTFMFGGH DTTASGLSWI LYNLAKHPEY QERCRQEVRE 370 380 390 400 410 420 LLRDREPEEI EWDDLAQLPF LTMCIKESLR LHPPATAISR CCTQDIMLPD GRVIPKGVIC 430 440 450 460 470 480 RISIFGTHHN PAVWPDPEVY NPFRFDADNG KGRSPLAFIP FSAGPRNCIG QTFAMSEMKV 490 500 510 520 ALALTLLRFR VLPDDKEPRR KPELILRAEG GLWLRVEPLS AGAH

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC101918 mRNA. Translation: AAI01919.1.
IPI	IPI00358227.
RefSeq	NP_001028858.1.
UniGene	Rn.215683
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000015751. Rattus norvegicus. [Contig view]
GeneID	290623.
KEGG	rno:290623.
Organism-specific databases
RGD	1305261. Cyp4f18.
Phylogenomic databases
HOVERGEN	Q3MID2.
Enzyme and pathway databases
BRENDA	1.14.13.30. 248.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	631306.

Entry information

Entry name

CP4F3_RAT

Accession

Primary (citable) accession number: Q3MID2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)