Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q3MI05 (PPGB_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysosomal protective protein

EC=3.4.16.5
Alternative name(s):
Cathepsin A
Gene names
Name:CTSA
Synonyms:PPGB
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins By similarity.

Catalytic activity

Release of a C-terminal amino acid with broad specificity.

Subunit structure

Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked By similarity.

Subcellular location

Lysosome By similarity.

Sequence similarities

Belongs to the peptidase S10 family.

Ontologies

Keywords
   Cellular componentLysosome
   DomainSignal
   Molecular functionCarboxypeptidase
Hydrolase
Protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionserine-type carboxypeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 By similarity
Chain28 – 479452Lysosomal protective protein
PRO_0000236219
Chain28 – 325298Lysosomal protective protein 32 kDa chain By similarity
PRO_0000236220
Chain326 – 479154Lysosomal protective protein 20 kDa chain By similarity
PRO_0000236221

Sites

Active site1771 By similarity
Active site3991 By similarity
Active site4561 By similarity

Amino acid modifications

Glycosylation1441N-linked (GlcNAc...) Potential
Glycosylation3321N-linked (GlcNAc...) Potential
Disulfide bond87 ↔ 361 By similarity
Disulfide bond239 ↔ 255 By similarity
Disulfide bond240 ↔ 245 By similarity
Disulfide bond280 ↔ 330 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3MI05 [UniParc].

Last modified October 25, 2005. Version 1.
Checksum: A37CC37D5C575FBF

FASTA47953,980
        10         20         30         40         50         60 
MFRAALWPPV LLLLQLLLLA CAPGGEGAHD QDEIRFLPGL AKQPSFRQYS GYLKGSGSKR 

        70         80         90        100        110        120 
LHYWFVESQK DPKSSPVVLW LNGGPGCSSL DGLLTEHGPF LIQPDGVTLE YNPYSWNLIA 

       130        140        150        160        170        180 
NVLYLESPAG VGFSYSDDKS YATNDTEVAQ SNFEALKDFF CLFPEYKGNE LFLTGESYAG 

       190        200        210        220        230        240 
IYIPTLAVLV MQDPSMNLQG LAVGNGLSSY EQNDNSLVYF AYYHGLLGNR LWSSLQTHCC 

       250        260        270        280        290        300 
SQNQCNFHDN KEPECVANLQ EVSHIVASSG LNIYNLYAPC AGGVPSHVRH EKDTVVVQDL 

       310        320        330        340        350        360 
GNIFTRLPLK RVWHQTLLRS GEKVHLDPPC TNTTAASNYL NDPHVRKALH IPEQLPRWDL 

       370        380        390        400        410        420 
CNFLVNIQYR RLYQSMCSQY LKLLSAQKYR ILLYNGDVDM ACNFMGDEWF VDSLNQKMEV 

       430        440        450        460        470 
QRRPWLVDYG ESGEQIAGFV KEFSHIAFLT IKGAGHMVPT DKPQAALTMF SRFLNRQPY 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Uterus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC104495 mRNA. Translation: AAI04496.1.
RefSeqNP_001030403.1. NM_001035326.1.
UniGeneBt.21410.

3D structure databases

ProteinModelPortalQ3MI05.
SMRQ3MI05. Positions 28-479.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS10.002.

Proteomic databases

PRIDEQ3MI05.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000039203; ENSBTAP00000039003; ENSBTAG00000027337.
GeneID518169.
KEGGbta:518169.

Organism-specific databases

CTD5476.

Phylogenomic databases

eggNOGCOG2939.
GeneTreeENSGT00560000077221.
HOGENOMHOG000198295.
HOVERGENHBG053652.
KOK13289.
OMAFYYFIVS.
OrthoDBEOG7KQ21H.
TreeFamTF323769.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERPTHR11802. PTHR11802. 1 hit.
PfamPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSPR00724. CRBOXYPTASEC.
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20872600.

Entry information

Entry namePPGB_BOVIN
AccessionPrimary (citable) accession number: Q3MI05
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: October 25, 2005
Last modified: June 11, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries