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Protein

Lysosomal protective protein

Gene

CTSA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins (By similarity).By similarity

Catalytic activityi

Release of a C-terminal amino acid with broad specificity.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771By similarity
Active sitei399 – 3991By similarity
Active sitei456 – 4561By similarity

GO - Molecular functioni

  1. serine-type carboxypeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Enzyme and pathway databases

ReactomeiREACT_204575. Glycosphingolipid metabolism.
REACT_213420. Sialic acid metabolism.

Protein family/group databases

MEROPSiS10.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal protective protein (EC:3.4.16.5)
Alternative name(s):
Cathepsin A
Cleaved into the following 2 chains:
Gene namesi
Name:CTSA
Synonyms:PPGB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 13

Subcellular locationi

Lysosome By similarity

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. lysosome Source: UniProtKB-SubCell
  3. membrane Source: Ensembl
  4. mitochondrion Source: Ensembl
  5. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727By similarityAdd
BLAST
Chaini28 – 479452Lysosomal protective proteinPRO_0000236219Add
BLAST
Chaini28 – 325298Lysosomal protective protein 32 kDa chainBy similarityPRO_0000236220Add
BLAST
Chaini326 – 479154Lysosomal protective protein 20 kDa chainBy similarityPRO_0000236221Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi87 ↔ 361By similarity
Glycosylationi144 – 1441N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi239 ↔ 255By similarity
Disulfide bondi240 ↔ 245By similarity
Disulfide bondi280 ↔ 330By similarity
Glycosylationi332 – 3321N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ3MI05.

Interactioni

Subunit structurei

Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ3MI05.
SMRiQ3MI05. Positions 28-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2939.
GeneTreeiENSGT00770000120552.
HOGENOMiHOG000198295.
HOVERGENiHBG053652.
InParanoidiQ3MI05.
KOiK13289.
OMAiQYRRIYQ.
OrthoDBiEOG7KQ21H.
TreeFamiTF323769.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3MI05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRAALWPPV LLLLQLLLLA CAPGGEGAHD QDEIRFLPGL AKQPSFRQYS
60 70 80 90 100
GYLKGSGSKR LHYWFVESQK DPKSSPVVLW LNGGPGCSSL DGLLTEHGPF
110 120 130 140 150
LIQPDGVTLE YNPYSWNLIA NVLYLESPAG VGFSYSDDKS YATNDTEVAQ
160 170 180 190 200
SNFEALKDFF CLFPEYKGNE LFLTGESYAG IYIPTLAVLV MQDPSMNLQG
210 220 230 240 250
LAVGNGLSSY EQNDNSLVYF AYYHGLLGNR LWSSLQTHCC SQNQCNFHDN
260 270 280 290 300
KEPECVANLQ EVSHIVASSG LNIYNLYAPC AGGVPSHVRH EKDTVVVQDL
310 320 330 340 350
GNIFTRLPLK RVWHQTLLRS GEKVHLDPPC TNTTAASNYL NDPHVRKALH
360 370 380 390 400
IPEQLPRWDL CNFLVNIQYR RLYQSMCSQY LKLLSAQKYR ILLYNGDVDM
410 420 430 440 450
ACNFMGDEWF VDSLNQKMEV QRRPWLVDYG ESGEQIAGFV KEFSHIAFLT
460 470
IKGAGHMVPT DKPQAALTMF SRFLNRQPY
Length:479
Mass (Da):53,980
Last modified:October 25, 2005 - v1
Checksum:iA37CC37D5C575FBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104495 mRNA. Translation: AAI04496.1.
RefSeqiNP_001030403.1. NM_001035326.1.
UniGeneiBt.21410.

Genome annotation databases

EnsembliENSBTAT00000039203; ENSBTAP00000039003; ENSBTAG00000027337.
GeneIDi518169.
KEGGibta:518169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104495 mRNA. Translation: AAI04496.1.
RefSeqiNP_001030403.1. NM_001035326.1.
UniGeneiBt.21410.

3D structure databases

ProteinModelPortaliQ3MI05.
SMRiQ3MI05. Positions 28-479.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS10.002.

Proteomic databases

PRIDEiQ3MI05.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000039203; ENSBTAP00000039003; ENSBTAG00000027337.
GeneIDi518169.
KEGGibta:518169.

Organism-specific databases

CTDi5476.

Phylogenomic databases

eggNOGiCOG2939.
GeneTreeiENSGT00770000120552.
HOGENOMiHOG000198295.
HOVERGENiHBG053652.
InParanoidiQ3MI05.
KOiK13289.
OMAiQYRRIYQ.
OrthoDBiEOG7KQ21H.
TreeFamiTF323769.

Enzyme and pathway databases

ReactomeiREACT_204575. Glycosphingolipid metabolism.
REACT_213420. Sialic acid metabolism.

Miscellaneous databases

NextBioi20872600.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiPPGB_BOVIN
AccessioniPrimary (citable) accession number: Q3MI05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: October 25, 2005
Last modified: January 7, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.