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Protein

Cytosolic Fe-S cluster assembly factor NUBP2

Gene

NUBP2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.UniRule annotationBy similarity

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi196 – 1961Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi199 – 1991Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 298ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP2UniRule annotation
Alternative name(s):
Nucleotide-binding protein 2UniRule annotation
Short name:
NBP 2UniRule annotation
Gene namesi
Name:NUBP2UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Subcellular locationi

  • Nucleus UniRule annotation
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome UniRule annotation
  • Cytoplasm By similarity
  • Cytoplasmcytoskeletoncilium axoneme By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing center By similarity

  • Note: Enriched at the centrosomes during mitosis. Enriched in centrioles of microtubule asters during prophase, prometaphase and telophase stages of mitosis (By similarity). Localized at centrioles and in the nucleus at interphase (By similarity). Colocalizes with nubp-1 at prometaphase (By similarity).UniRule annotationBy similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Cytosolic Fe-S cluster assembly factor NUBP2PRO_0000288716Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineUniRule annotationBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ3MHY6.

Expressioni

Gene expression databases

ExpressionAtlasiQ3MHY6. baseline.

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1. Interacts with NUBP1.UniRule annotationBy similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022029.

Structurei

3D structure databases

ProteinModelPortaliQ3MHY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
GeneTreeiENSGT00550000075057.
HOGENOMiHOG000079916.
HOVERGENiHBG051027.
InParanoidiQ3MHY6.
OMAiDVLWDET.
OrthoDBiEOG7D59NZ.
TreeFamiTF354321.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35.
MF_03039. NUBP2.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028600. NUBP2/Cfd1_eukaryotes.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3MHY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAAAEPGNL AGVRHIVLVL SGKGGVGKST ISTELALALR HAGKKVGILD
60 70 80 90 100
VDLCGPSIPR MLRAQGRAVH QGDSGWVPVF VDREQSISLM SVGFLLEQPD
110 120 130 140 150
EAVVWRGPKK NALIKQFVSD VAWGQLDYLL VDTPPGTSDE HMAVVDALRP
160 170 180 190 200
HSPLGALVVT TPQAVSVGDV RRELTFCRKV GLRVIGLVEN MSGFVCPHCS
210 220 230 240 250
ECTNVFSKGG GEELARHAGV PFLGSVPLDP ELTRSLEDGR DFIQDFPDSP
260 270
AFPALSSIAQ KILSETPAGL S
Length:271
Mass (Da):28,789
Last modified:October 25, 2005 - v1
Checksum:iF02C30153981F90C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104526 mRNA. Translation: AAI04527.1.
RefSeqiNP_001029677.1. NM_001034505.1.
UniGeneiBt.2198.

Genome annotation databases

EnsembliENSBTAT00000022029; ENSBTAP00000022029; ENSBTAG00000016561.
GeneIDi515660.
KEGGibta:515660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104526 mRNA. Translation: AAI04527.1.
RefSeqiNP_001029677.1. NM_001034505.1.
UniGeneiBt.2198.

3D structure databases

ProteinModelPortaliQ3MHY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022029.

Proteomic databases

PaxDbiQ3MHY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000022029; ENSBTAP00000022029; ENSBTAG00000016561.
GeneIDi515660.
KEGGibta:515660.

Organism-specific databases

CTDi10101.

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
GeneTreeiENSGT00550000075057.
HOGENOMiHOG000079916.
HOVERGENiHBG051027.
InParanoidiQ3MHY6.
OMAiDVLWDET.
OrthoDBiEOG7D59NZ.
TreeFamiTF354321.

Miscellaneous databases

NextBioi20871944.

Gene expression databases

ExpressionAtlasiQ3MHY6. baseline.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35.
MF_03039. NUBP2.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028600. NUBP2/Cfd1_eukaryotes.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.

Entry informationi

Entry nameiNUBP2_BOVIN
AccessioniPrimary (citable) accession number: Q3MHY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 25, 2005
Last modified: January 20, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.