Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial

Gene

SUCLG2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.UniRule annotation

Catalytic activityi

GTP + succinate + CoA = GDP + phosphate + succinyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial (SUCLG2), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2), Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (SUCLG1), Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (SUCLG1), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57GTPUniRule annotation1
Sitei79Important for substrate specificityUniRule annotation1
Binding sitei146GTP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Sitei147Important for substrate specificityUniRule annotation1
Metal bindingi243MagnesiumUniRule annotation1
Metal bindingi257MagnesiumUniRule annotation1
Binding sitei308Substrate; shared with subunit alphaUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi90 – 92GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processTricarboxylic acid cycle
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-71403 Citric acid cycle (TCA cycle)
UniPathwayiUPA00223; UER00999

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrialUniRule annotation (EC:6.2.1.4UniRule annotation)
Alternative name(s):
GTP-specific succinyl-CoA synthetase subunit betaUniRule annotation
Short name:
G-SCSUniRule annotation
Short name:
GTPSCSUniRule annotation
Succinyl-CoA synthetase beta-G chainUniRule annotation
Short name:
SCS-betaGUniRule annotation
Gene namesi
Name:SUCLG2UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 22

Organism-specific databases

VGNCiVGNC:35457 SUCLG2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37MitochondrionBy similarityAdd BLAST37
ChainiPRO_000023098938 – 432Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrialAdd BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73N6-acetyllysineBy similarity1
Modified residuei78N6-succinyllysineBy similarity1
Modified residuei111N6-acetyllysineBy similarity1
Modified residuei132N6-acetyllysineBy similarity1
Modified residuei139N6-acetyllysineBy similarity1
Modified residuei161PhosphoserineBy similarity1
Modified residuei200N6-acetyllysineBy similarity1
Modified residuei218N6-acetyllysineBy similarity1
Modified residuei227N6-acetyllysineBy similarity1
Modified residuei271N6-acetyllysineBy similarity1
Modified residuei338N6-succinyllysineBy similarity1
Modified residuei347N6-acetyllysineBy similarity1
Modified residuei386N6-acetyllysineBy similarity1
Modified residuei423N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ3MHX5
PeptideAtlasiQ3MHX5
PRIDEiQ3MHX5

Expressioni

Gene expression databases

BgeeiENSBTAG00000009541

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012551

Structurei

3D structure databases

ProteinModelPortaliQ3MHX5
SMRiQ3MHX5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 274ATP-graspUniRule annotationAdd BLAST229

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni365 – 367Substrate binding; shared with subunit alphaUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2799 Eukaryota
COG0045 LUCA
GeneTreeiENSGT00390000010170
HOGENOMiHOG000007059
HOVERGENiHBG055555
InParanoidiQ3MHX5
KOiK01900
OMAiVQIEINP
OrthoDBiEOG091G07T9
TreeFamiTF300624

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.40.50.261, 1 hit
HAMAPiMF_00558 Succ_CoA_beta, 1 hit
MF_03221 Succ_CoA_betaG_euk, 1 hit
InterProiView protein in InterPro
IPR013650 ATP-grasp_succ-CoA_synth-type
IPR013815 ATP_grasp_subdomain_1
IPR005811 CoA_ligase
IPR017866 Succ-CoA_synthase_bsu_CS
IPR034722 Succ_CoA_betaG_euk
IPR005809 Succ_CoA_synthase_bsu
IPR016102 Succinyl-CoA_synth-like
PANTHERiPTHR11815 PTHR11815, 1 hit
PfamiView protein in Pfam
PF08442 ATP-grasp_2, 1 hit
PF00549 Ligase_CoA, 1 hit
PIRSFiPIRSF001554 SucCS_beta, 1 hit
SUPFAMiSSF52210 SSF52210, 1 hit
TIGRFAMsiTIGR01016 sucCoAbeta, 1 hit
PROSITEiView protein in PROSITE
PS01217 SUCCINYL_COA_LIG_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3MHX5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPVGAQAR KLLRDLVLRP PLLAARSQVV QLTSRRWLNL QEYQSKKLMS
60 70 80 90 100
DNGVKVQRFF VADTANEALE AAKRLNAKEI VLKAQILAGG RGKGVFSSGL
110 120 130 140 150
KGGVHLTKDP KVVGQLAKQM IGYNLATKQT PKEGVKVKKV MVAEALDISR
160 170 180 190 200
ETYLAILMDR SCNGPVLVGS PQGGVDIEEV AASNPELIFK EQIDIIEGIK
210 220 230 240 250
DSQAQRMAEN LGFLGPLKNQ AADQIKKLYN LFLKIDATQV EVNPFGETPE
260 270 280 290 300
GQVVCFDAKI NFDDNAEFRQ KDIFAMDDKS ENEPIENEAA RYDLKYIGLD
310 320 330 340 350
GNIACFVNGA GLAMATCDII FLNGGKPANF LDLGGGVKES QVYQAFKLLT
360 370 380 390 400
ADPKVEAILV NIFGGIVNCA IIANGITKAC RELELKVPLV VRLEGTNVHE
410 420 430
AQNILSNSGL PITSAVDLED AAKKAVASVA KK
Length:432
Mass (Da):46,691
Last modified:October 25, 2005 - v1
Checksum:iC3A5343FF0A65B3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104559 mRNA Translation: AAI04560.1
RefSeqiNP_001029811.1, NM_001034639.1
UniGeneiBt.23260

Genome annotation databases

EnsembliENSBTAT00000012551; ENSBTAP00000012551; ENSBTAG00000009541
GeneIDi537713
KEGGibta:537713

Similar proteinsi

Entry informationi

Entry nameiSUCB2_BOVIN
AccessioniPrimary (citable) accession number: Q3MHX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 25, 2005
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health