ID   NB5R1_BOVIN             Reviewed;         305 AA.
AC   Q3MHW9;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   16-JUN-2009, entry version 29.
DE   RecName: Full=NADH-cytochrome b5 reductase 1;
DE            Short=b5R.1;
DE            EC=1.6.2.2;
GN   Name=CYB5R1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Ascending colon;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: NADH-cytochrome b5 reductases are involved in
CC       desaturation and elongation of fatty acids, cholesterol
CC       biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin
CC       reduction (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADH + 2 ferricytochrome b5 = NAD(+) + H(+) +
CC       2 ferrocytochrome b5.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein
CC       (Potential).
CC   -!- SIMILARITY: Belongs to the flavoprotein pyridine nucleotide
CC       cytochrome reductase family.
CC   -!- SIMILARITY: Contains 1 FAD-binding FR-type domain.
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DR   EMBL; BC104584; AAI04585.1; -; mRNA.
DR   IPI; IPI00689803; -.
DR   RefSeq; NP_001029690.1; -.
DR   UniGene; Bt.997; -.
DR   Ensembl; ENSBTAG00000019927; Bos taurus.
DR   GeneID; 516287; -.
DR   KEGG; bta:516287; -.
DR   HOVERGEN; Q3MHW9; -.
DR   OMA; Q3MHW9; LWFTLDH.
DR   BRENDA; 1.6.2.2; 251.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004128; F:cytochrome-b5 reductase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR017927; Fd_Rdtase_FAD-bd.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR001834; NADH-Cyt_B5_reductase.
DR   InterPro; IPR008333; OxRdtase_FAD-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD_bd.
DR   Pfam; PF00970; FAD_binding_6; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PRINTS; PR00406; CYTB5RDTASE.
DR   PRINTS; PR00371; FPNCR.
DR   PROSITE; PS51384; FAD_FR; 1.
PE   2: Evidence at transcript level;
KW   FAD; Flavoprotein; Lipid synthesis; Membrane; NAD; Oxidoreductase;
KW   Steroid biosynthesis; Sterol biosynthesis; Transmembrane.
FT   CHAIN         1    305       NADH-cytochrome b5 reductase 1.
FT                                /FTId=PRO_0000287544.
FT   TRANSMEM      8     28       Potential.
FT   DOMAIN       44    156       FAD-binding FR-type.
FT   NP_BIND     136    166       FAD (By similarity).
FT   NP_BIND     175    210       FAD (By similarity).
SQ   SEQUENCE   305 AA;  33861 MW;  BC12CA38311828F4 CRC64;
     MGLQPSPVLL ASLGVGLLTL LGVALGAYLV RRSRRPPVTL LDPNEKYQLR LLDKTTVNHN
     TKRFRFALPT AHHVLGLPVG KHVYLSARID GSLVIRPYTP VTSDEDQGYV DLVIKVYLKG
     VHPKFPEGGK MSQYLDSLKI GDVVEFRGPS GLLTYAGKGK FNIQPNKKAP PEARVARNLG
     MIAGGTGITP MLQLIRAILK DPEDPTQCFL LFANQTEKDI ILREDLEELQ ARHPGRFKLW
     FTLDHPPEGW AYSKGFVSVD MIREHLPAPG EDVLLLLCGP PPMVQLACHP SLDKLGYSPK
     MRFTY
//