Reviewed, UniProtKB/Swiss-Prot Q3MHW9 (NB5R1_BOVIN)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 1
      Short name=b5R.1
    EC=1.6.2.2

Gene names

Name:

CYB5R1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	NADH-cytochrome b5 reductase 1		PRO_0000287544
Regions
	Transmembrane	8 – 28	21	Potential
	Domain	44 – 156	113	FAD-binding FR-type
	Nucleotide binding	136 – 166	31	FAD By similarity
	Nucleotide binding	175 – 210	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MHW9-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: BC12CA38311828F4	FASTA	305	33,861
	10 20 30 40 50 60 MGLQPSPVLL ASLGVGLLTL LGVALGAYLV RRSRRPPVTL LDPNEKYQLR LLDKTTVNHN 70 80 90 100 110 120 TKRFRFALPT AHHVLGLPVG KHVYLSARID GSLVIRPYTP VTSDEDQGYV DLVIKVYLKG 130 140 150 160 170 180 VHPKFPEGGK MSQYLDSLKI GDVVEFRGPS GLLTYAGKGK FNIQPNKKAP PEARVARNLG 190 200 210 220 230 240 MIAGGTGITP MLQLIRAILK DPEDPTQCFL LFANQTEKDI ILREDLEELQ ARHPGRFKLW 250 260 270 280 290 300 FTLDHPPEGW AYSKGFVSVD MIREHLPAPG EDVLLLLCGP PPMVQLACHP SLDKLGYSPK MRFTY

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Ascending colon.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC104584 mRNA. Translation: AAI04585.1.
IPI	IPI00689803.
RefSeq	NP_001029690.1.
UniGene	Bt.997
3D structure databases
SMR	Q3MHW9. Positions 36-302.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000026548; ENSBTAP00000026548; ENSBTAG00000019927; Bos taurus. [Genome view]
GeneID	516287.
KEGG	bta:516287.
Organism-specific databases
CTD	516287.
Phylogenomic databases
eggNOG	maNOG05378.
HOVERGEN	Q3MHW9.
InParanoid	Q3MHW9.
OMA	HPPKDWA.
OrthoDB	EOG9KM1NT.
PhylomeDB	Q3MHW9.
Enzyme and pathway databases
BRENDA	1.6.2.2. 251.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD_bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NB5R1_BOVIN

Accession

Primary (citable) accession number: Q3MHW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	October 25, 2005
Last modified:	January 19, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)