ID   TUT1_RAT                Reviewed;         866 AA.
AC   Q3MHT4;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   16-JUN-2009, entry version 32.
DE   RecName: Full=U6 snRNA-specific terminal uridylyltransferase 1;
DE            Short=U6-TUTase;
DE            EC=2.7.7.52;
DE   AltName: Full=RNA-binding motif protein 21;
DE            Short=RNA-binding protein 21;
GN   Name=Tut1; Synonyms=Rbm21;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Highly specific terminal uridylyltransferase that
CC       exclusively accepts U6 snRNA as substrate. U6 snRNA is unique in
CC       that nucleotides are both added to and removed from its 3'-end.
CC       U6-TUTase is responsible for a controlled elongation reaction that
CC       results in the restoration of the four 3'-terminal UMP-residues
CC       found in newly transcribed U6 snRNA (By similarity).
CC   -!- CATALYTIC ACTIVITY: UTP + RNA(n) = diphosphate + RNA(n+1).
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus (By similarity).
CC   -!- PTM: Phosphorylated upon DNA damage, probably by ATM or ATR (By
CC       similarity).
CC   -!- SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.
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DR   EMBL; BC104695; AAI04696.1; -; mRNA.
DR   IPI; IPI00371444; -.
DR   RefSeq; NP_001029073.1; -.
DR   UniGene; Rn.129389; -.
DR   PhosphoSite; Q3MHT4; -.
DR   Ensembl; ENSRNOG00000020047; Rattus norvegicus.
DR   GeneID; 499314; -.
DR   KEGG; rno:499314; -.
DR   NMPDR; fig|10116.3.peg.3665; -.
DR   RGD; 1561043; Tut1.
DR   HOVERGEN; Q3MHT4; -.
DR   OMA; Q3MHT4; QGGTSKR.
DR   BRENDA; 2.7.7.52; 248.
DR   NextBio; 702524; -.
DR   ArrayExpress; Q3MHT4; -.
DR   GermOnline; ENSRNOG00000020047; Rattus norvegicus.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0050265; F:RNA uridylyltransferase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   InterPro; IPR012677; a_b_plait_nuc_bd.
DR   InterPro; IPR002058; PAP_assoc.
DR   InterPro; IPR000504; RRM_RNP1.
DR   InterPro; IPR015880; Znf_C2H2-like.
DR   Gene3D; G3DSA:3.30.70.330; a_b_plait_nuc_bd; 1.
DR   Pfam; PF03828; PAP_assoc; 1.
DR   Pfam; PF00076; RRM_1; 1.
DR   SMART; SM00360; RRM; 1.
DR   SMART; SM00355; ZnF_C2H2; 1.
DR   PROSITE; PS50102; RRM; 1.
PE   2: Evidence at transcript level;
KW   Nucleotidyltransferase; Nucleus; Phosphoprotein; RNA-binding;
KW   Transferase.
FT   CHAIN         1    866       U6 snRNA-specific terminal
FT                                uridylyltransferase 1.
FT                                /FTId=PRO_0000254188.
FT   DOMAIN       56    128       RRM.
FT   MOD_RES     741    741       Phosphoserine (By similarity).
SQ   SEQUENCE   866 AA;  94378 MW;  E85DE9E051D30412 CRC64;
     MAAVDSDVVS LPRGRFRCCL CDVTTANRPS LDAHLKGRKH RDLVQLRATR KAQGLRSVFV
     SGFPRDVGSA QLSEYFQTFG PVANIVMDKD KGVFAIVEMG DISAREAVLS QPKHSLGGHT
     LRVRPREQKE FQSPASKSPK GVDSNSHQLA QALAEAADVG AQMVKLVELR ELSEAERQLR
     TLVVALMQEV FTEFFPGCVV HPFGSSVNSF DVHGCDLDLF LDLGDMEEPQ PDPQTPKLPE
     ASSLDSTLAS SLDPQVLACT PASLDSLSPT SLQDSEALDF ETPSSLAPQT PDSALGSDTV
     TSPQSLPPVS PLEEDRGEGK HRKELELAEA SKDEKEEATA VLELVGSILR GCVPGVYRVQ
     TVPSARRPVV KFCHRPSGLH GDISLSNRLA LYNSRFLNLC SEMDSRVRPL VYTLRCWAQH
     NGLSGGGPLL NNYALTLLVI YFLQTRDPPV LPTVAQLTQR SGEGEQVEVD GWDCSFPKDA
     SRLEPSTNVE PLSSLLAQFF SCVSCWDLSG SLLSLREGQA LMVAGGLPSD LWEGLRLGPM
     NLQDPFDLSH NVAANVTSRV AKRLQSSCGA AASYCRSLQY QQRSSRGRDW GLLPLLQPSS
     PSSLLSAKLI PLPSAPFPQI ITALVSVLRE ALGCHIEQGT KRRRSEGARS KDSPLGGANK
     RPRLSGQEKS CEEGKEEPQG CAGDHSENEV EEMVIELRET PQDWALLHCG PPGELPLMTA
     KCLDKTAEQN PMEPEGAGEG SPGETEKEAS HPSSVSWRCA LWHQIWQGRR RARRRFQQQT
     KEEGRGGPST GAEWLAVEAR VTQELKGPKS EQQRLQGEPL LTFVASASQA EQTLTVAPLQ
     DPQGLFPGLH HFLQVFIPQA LKNLLK
//