Reviewed, UniProtKB/Swiss-Prot Q3MHT4 (TUT1_RAT)

Names and origin

Protein names

Recommended name:
    U6 snRNA-specific terminal uridylyltransferase 1
      Short name=U6-TUTase
    EC=2.7.7.52
Alternative name(s):
    RNA-binding motif protein 21
      Short name=RNA-binding protein 21

Gene names

Name:	Tut1
Synonyms:	Rbm21

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	866 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Highly specific terminal uridylyltransferase that exclusively accepts U6 snRNA as substrate. U6 snRNA is unique in that nucleotides are both added to and removed from its 3'-end. U6-TUTase is responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA By similarity.
Catalytic activity	UTP + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Nucleus › nucleolus By similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA uridylyltransferase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 866	866	U6 snRNA-specific terminal uridylyltransferase 1		PRO_0000254188
Regions
	Domain	56 – 128	73	RRM
Amino acid modifications
	Modified residue	741	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MHT4-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: E85DE9E051D30412	FASTA	866	94,378
	10 20 30 40 50 60 MAAVDSDVVS LPRGRFRCCL CDVTTANRPS LDAHLKGRKH RDLVQLRATR KAQGLRSVFV 70 80 90 100 110 120 SGFPRDVGSA QLSEYFQTFG PVANIVMDKD KGVFAIVEMG DISAREAVLS QPKHSLGGHT 130 140 150 160 170 180 LRVRPREQKE FQSPASKSPK GVDSNSHQLA QALAEAADVG AQMVKLVELR ELSEAERQLR 190 200 210 220 230 240 TLVVALMQEV FTEFFPGCVV HPFGSSVNSF DVHGCDLDLF LDLGDMEEPQ PDPQTPKLPE 250 260 270 280 290 300 ASSLDSTLAS SLDPQVLACT PASLDSLSPT SLQDSEALDF ETPSSLAPQT PDSALGSDTV 310 320 330 340 350 360 TSPQSLPPVS PLEEDRGEGK HRKELELAEA SKDEKEEATA VLELVGSILR GCVPGVYRVQ 370 380 390 400 410 420 TVPSARRPVV KFCHRPSGLH GDISLSNRLA LYNSRFLNLC SEMDSRVRPL VYTLRCWAQH 430 440 450 460 470 480 NGLSGGGPLL NNYALTLLVI YFLQTRDPPV LPTVAQLTQR SGEGEQVEVD GWDCSFPKDA 490 500 510 520 530 540 SRLEPSTNVE PLSSLLAQFF SCVSCWDLSG SLLSLREGQA LMVAGGLPSD LWEGLRLGPM 550 560 570 580 590 600 NLQDPFDLSH NVAANVTSRV AKRLQSSCGA AASYCRSLQY QQRSSRGRDW GLLPLLQPSS 610 620 630 640 650 660 PSSLLSAKLI PLPSAPFPQI ITALVSVLRE ALGCHIEQGT KRRRSEGARS KDSPLGGANK 670 680 690 700 710 720 RPRLSGQEKS CEEGKEEPQG CAGDHSENEV EEMVIELRET PQDWALLHCG PPGELPLMTA 730 740 750 760 770 780 KCLDKTAEQN PMEPEGAGEG SPGETEKEAS HPSSVSWRCA LWHQIWQGRR RARRRFQQQT 790 800 810 820 830 840 KEEGRGGPST GAEWLAVEAR VTQELKGPKS EQQRLQGEPL LTFVASASQA EQTLTVAPLQ 850 860 DPQGLFPGLH HFLQVFIPQA LKNLLK

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC104695 mRNA. Translation: AAI04696.1.
IPI	IPI00371444.
RefSeq	NP_001029073.1.
UniGene	Rn.129389
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q3MHT4.
Genome annotation databases
Ensembl	ENSRNOG00000020047. Rattus norvegicus. [Contig view]
GeneID	499314.
KEGG	rno:499314.
NMPDR	fig|10116.3.peg.3665.
Organism-specific databases
RGD	1561043. Tut1.
Phylogenomic databases
HOVERGEN	Q3MHT4.
OMA	Q3MHT4. QGGTSKR.
Enzyme and pathway databases
BRENDA	2.7.7.52. 248.
Gene expression databases
ArrayExpress	Q3MHT4.
GermOnline	ENSRNOG00000020047. Rattus norvegicus.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR002058. PAP_assoc. IPR000504. RRM_RNP1. IPR015880. Znf_C2H2-like. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
Pfam	PF03828. PAP_assoc. 1 hit. PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. SM00355. ZnF_C2H2. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	702524.

Entry information

Entry name

TUT1_RAT

Accession

Primary (citable) accession number: Q3MHT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)