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Q3MHT4

- STPAP_RAT

UniProt

Q3MHT4 - STPAP_RAT

Protein

Speckle targeted PIP5K1A-regulated poly(A) polymerase

Gene

Tut1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (25 Oct 2005)
      Previous versions | rss
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    Functioni

    Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation By similarity.By similarity

    Catalytic activityi

    UTP + RNA(n) = diphosphate + RNA(n+1).
    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Magnesium or manganese.By similarity

    Enzyme regulationi

    Adenylyltransferase activity is specifically phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi216 – 2161Magnesium or manganese; catalyticBy similarity
    Metal bindingi218 – 2181Magnesium or manganese; catalyticBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri16 – 4025C2H2-typeAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. mRNA 3'-UTR binding Source: UniProtKB
    4. polynucleotide adenylyltransferase activity Source: UniProtKB
    5. RNA binding Source: UniProtKB
    6. RNA uridylyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. mRNA cleavage Source: UniProtKB
    2. mRNA polyadenylation Source: UniProtKB
    3. snRNA processing Source: UniProtKB

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
    Short name:
    Star-PAP
    Alternative name(s):
    RNA-binding motif protein 21
    Short name:
    RNA-binding protein 21
    U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
    Short name:
    U6-TUTase
    Gene namesi
    Name:Tut1
    Synonyms:Rbm21
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi1561043. Tut1.

    Subcellular locationi

    Nucleusnucleolus By similarity. Nucleus speckle By similarity

    GO - Cellular componenti

    1. nuclear speck Source: UniProtKB
    2. nucleolus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 866866Speckle targeted PIP5K1A-regulated poly(A) polymerasePRO_0000254188Add
    BLAST

    Post-translational modificationi

    Phosphorylated by CK1 in the proline-rich (Pro-rich) region.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ3MHT4.
    PRIDEiQ3MHT4.

    PTM databases

    PhosphoSiteiQ3MHT4.

    Expressioni

    Gene expression databases

    GenevestigatoriQ3MHT4.

    Interactioni

    Subunit structurei

    Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A; interaction By similarity.By similarity

    Protein-protein interaction databases

    BioGridi270623. 1 interaction.
    IntActiQ3MHT4. 1 interaction.
    STRINGi10116.ENSRNOP00000027187.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3MHT4.
    SMRiQ3MHT4. Positions 55-141.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini56 – 12873RRMPROSITE-ProRule annotationAdd
    BLAST
    Domaini492 – 55059PAP-associatedAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi229 – 31183Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B-like family.Curated
    Contains 1 C2H2-type zinc finger.Curated
    Contains 1 PAP-associated domain.Curated
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri16 – 4025C2H2-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5260.
    GeneTreeiENSGT00550000074490.
    HOVERGENiHBG079670.
    InParanoidiQ3MHT4.
    OMAiREALGCH.
    OrthoDBiEOG7353WD.
    PhylomeDBiQ3MHT4.
    TreeFamiTF354308.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR002058. PAP_assoc.
    IPR000504. RRM_dom.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF03828. PAP_assoc. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3MHT4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAVDSDVVS LPRGRFRCCL CDVTTANRPS LDAHLKGRKH RDLVQLRATR    50
    KAQGLRSVFV SGFPRDVGSA QLSEYFQTFG PVANIVMDKD KGVFAIVEMG 100
    DISAREAVLS QPKHSLGGHT LRVRPREQKE FQSPASKSPK GVDSNSHQLA 150
    QALAEAADVG AQMVKLVELR ELSEAERQLR TLVVALMQEV FTEFFPGCVV 200
    HPFGSSVNSF DVHGCDLDLF LDLGDMEEPQ PDPQTPKLPE ASSLDSTLAS 250
    SLDPQVLACT PASLDSLSPT SLQDSEALDF ETPSSLAPQT PDSALGSDTV 300
    TSPQSLPPVS PLEEDRGEGK HRKELELAEA SKDEKEEATA VLELVGSILR 350
    GCVPGVYRVQ TVPSARRPVV KFCHRPSGLH GDISLSNRLA LYNSRFLNLC 400
    SEMDSRVRPL VYTLRCWAQH NGLSGGGPLL NNYALTLLVI YFLQTRDPPV 450
    LPTVAQLTQR SGEGEQVEVD GWDCSFPKDA SRLEPSTNVE PLSSLLAQFF 500
    SCVSCWDLSG SLLSLREGQA LMVAGGLPSD LWEGLRLGPM NLQDPFDLSH 550
    NVAANVTSRV AKRLQSSCGA AASYCRSLQY QQRSSRGRDW GLLPLLQPSS 600
    PSSLLSAKLI PLPSAPFPQI ITALVSVLRE ALGCHIEQGT KRRRSEGARS 650
    KDSPLGGANK RPRLSGQEKS CEEGKEEPQG CAGDHSENEV EEMVIELRET 700
    PQDWALLHCG PPGELPLMTA KCLDKTAEQN PMEPEGAGEG SPGETEKEAS 750
    HPSSVSWRCA LWHQIWQGRR RARRRFQQQT KEEGRGGPST GAEWLAVEAR 800
    VTQELKGPKS EQQRLQGEPL LTFVASASQA EQTLTVAPLQ DPQGLFPGLH 850
    HFLQVFIPQA LKNLLK 866
    Length:866
    Mass (Da):94,378
    Last modified:October 25, 2005 - v1
    Checksum:iE85DE9E051D30412
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC104695 mRNA. Translation: AAI04696.1.
    RefSeqiNP_001029073.1. NM_001033901.1.
    UniGeneiRn.129389.

    Genome annotation databases

    EnsembliENSRNOT00000027187; ENSRNOP00000027187; ENSRNOG00000020047.
    GeneIDi499314.
    KEGGirno:499314.
    UCSCiRGD:1561043. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC104695 mRNA. Translation: AAI04696.1 .
    RefSeqi NP_001029073.1. NM_001033901.1.
    UniGenei Rn.129389.

    3D structure databases

    ProteinModelPortali Q3MHT4.
    SMRi Q3MHT4. Positions 55-141.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 270623. 1 interaction.
    IntActi Q3MHT4. 1 interaction.
    STRINGi 10116.ENSRNOP00000027187.

    PTM databases

    PhosphoSitei Q3MHT4.

    Proteomic databases

    PaxDbi Q3MHT4.
    PRIDEi Q3MHT4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027187 ; ENSRNOP00000027187 ; ENSRNOG00000020047 .
    GeneIDi 499314.
    KEGGi rno:499314.
    UCSCi RGD:1561043. rat.

    Organism-specific databases

    CTDi 64852.
    RGDi 1561043. Tut1.

    Phylogenomic databases

    eggNOGi COG5260.
    GeneTreei ENSGT00550000074490.
    HOVERGENi HBG079670.
    InParanoidi Q3MHT4.
    OMAi REALGCH.
    OrthoDBi EOG7353WD.
    PhylomeDBi Q3MHT4.
    TreeFami TF354308.

    Miscellaneous databases

    NextBioi 702524.
    PROi Q3MHT4.

    Gene expression databases

    Genevestigatori Q3MHT4.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR002058. PAP_assoc.
    IPR000504. RRM_dom.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiSTPAP_RAT
    AccessioniPrimary (citable) accession number: Q3MHT4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: October 25, 2005
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3