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Q3MHT4

- STPAP_RAT

UniProt

Q3MHT4 - STPAP_RAT

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Protein
Speckle targeted PIP5K1A-regulated poly(A) polymerase
Gene
Tut1, Rbm21
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation By similarity.

Catalytic activityi

UTP + RNA(n) = diphosphate + RNA(n+1).
ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Magnesium or manganese By similarity.

Enzyme regulationi

Adenylyltransferase activity is specifically phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi216 – 2161Magnesium or manganese; catalytic By similarity
Metal bindingi218 – 2181Magnesium or manganese; catalytic By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri16 – 4025C2H2-type
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB
  3. RNA uridylyltransferase activity Source: UniProtKB
  4. mRNA 3'-UTR binding Source: UniProtKB
  5. metal ion binding Source: UniProtKB-KW
  6. polynucleotide adenylyltransferase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. mRNA cleavage Source: UniProtKB
  2. mRNA polyadenylation Source: UniProtKB
  3. snRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
Short name:
Star-PAP
Alternative name(s):
RNA-binding motif protein 21
Short name:
RNA-binding protein 21
U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
Short name:
U6-TUTase
Gene namesi
Name:Tut1
Synonyms:Rbm21
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi1561043. Tut1.

Subcellular locationi

Nucleusnucleolus By similarity. Nucleus speckle By similarity

GO - Cellular componenti

  1. nuclear speck Source: UniProtKB
  2. nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 866866Speckle targeted PIP5K1A-regulated poly(A) polymerase
PRO_0000254188Add
BLAST

Post-translational modificationi

Phosphorylated by CK1 in the proline-rich (Pro-rich) region.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3MHT4.
PRIDEiQ3MHT4.

PTM databases

PhosphoSiteiQ3MHT4.

Expressioni

Gene expression databases

GenevestigatoriQ3MHT4.

Interactioni

Subunit structurei

Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A; interaction By similarity.

Protein-protein interaction databases

BioGridi270623. 1 interaction.
IntActiQ3MHT4. 1 interaction.
STRINGi10116.ENSRNOP00000027187.

Structurei

3D structure databases

ProteinModelPortaliQ3MHT4.
SMRiQ3MHT4. Positions 55-141.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 12873RRM
Add
BLAST
Domaini492 – 55059PAP-associated
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi229 – 31183Pro-rich
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5260.
GeneTreeiENSGT00550000074490.
HOVERGENiHBG079670.
InParanoidiQ3MHT4.
OMAiREALGCH.
OrthoDBiEOG7353WD.
PhylomeDBiQ3MHT4.
TreeFamiTF354308.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3MHT4-1 [UniParc]FASTAAdd to Basket

« Hide

MAAVDSDVVS LPRGRFRCCL CDVTTANRPS LDAHLKGRKH RDLVQLRATR    50
KAQGLRSVFV SGFPRDVGSA QLSEYFQTFG PVANIVMDKD KGVFAIVEMG 100
DISAREAVLS QPKHSLGGHT LRVRPREQKE FQSPASKSPK GVDSNSHQLA 150
QALAEAADVG AQMVKLVELR ELSEAERQLR TLVVALMQEV FTEFFPGCVV 200
HPFGSSVNSF DVHGCDLDLF LDLGDMEEPQ PDPQTPKLPE ASSLDSTLAS 250
SLDPQVLACT PASLDSLSPT SLQDSEALDF ETPSSLAPQT PDSALGSDTV 300
TSPQSLPPVS PLEEDRGEGK HRKELELAEA SKDEKEEATA VLELVGSILR 350
GCVPGVYRVQ TVPSARRPVV KFCHRPSGLH GDISLSNRLA LYNSRFLNLC 400
SEMDSRVRPL VYTLRCWAQH NGLSGGGPLL NNYALTLLVI YFLQTRDPPV 450
LPTVAQLTQR SGEGEQVEVD GWDCSFPKDA SRLEPSTNVE PLSSLLAQFF 500
SCVSCWDLSG SLLSLREGQA LMVAGGLPSD LWEGLRLGPM NLQDPFDLSH 550
NVAANVTSRV AKRLQSSCGA AASYCRSLQY QQRSSRGRDW GLLPLLQPSS 600
PSSLLSAKLI PLPSAPFPQI ITALVSVLRE ALGCHIEQGT KRRRSEGARS 650
KDSPLGGANK RPRLSGQEKS CEEGKEEPQG CAGDHSENEV EEMVIELRET 700
PQDWALLHCG PPGELPLMTA KCLDKTAEQN PMEPEGAGEG SPGETEKEAS 750
HPSSVSWRCA LWHQIWQGRR RARRRFQQQT KEEGRGGPST GAEWLAVEAR 800
VTQELKGPKS EQQRLQGEPL LTFVASASQA EQTLTVAPLQ DPQGLFPGLH 850
HFLQVFIPQA LKNLLK 866
Length:866
Mass (Da):94,378
Last modified:October 25, 2005 - v1
Checksum:iE85DE9E051D30412
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC104695 mRNA. Translation: AAI04696.1.
RefSeqiNP_001029073.1. NM_001033901.1.
UniGeneiRn.129389.

Genome annotation databases

EnsembliENSRNOT00000027187; ENSRNOP00000027187; ENSRNOG00000020047.
GeneIDi499314.
KEGGirno:499314.
UCSCiRGD:1561043. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC104695 mRNA. Translation: AAI04696.1 .
RefSeqi NP_001029073.1. NM_001033901.1.
UniGenei Rn.129389.

3D structure databases

ProteinModelPortali Q3MHT4.
SMRi Q3MHT4. Positions 55-141.
ModBasei Search...

Protein-protein interaction databases

BioGridi 270623. 1 interaction.
IntActi Q3MHT4. 1 interaction.
STRINGi 10116.ENSRNOP00000027187.

PTM databases

PhosphoSitei Q3MHT4.

Proteomic databases

PaxDbi Q3MHT4.
PRIDEi Q3MHT4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000027187 ; ENSRNOP00000027187 ; ENSRNOG00000020047 .
GeneIDi 499314.
KEGGi rno:499314.
UCSCi RGD:1561043. rat.

Organism-specific databases

CTDi 64852.
RGDi 1561043. Tut1.

Phylogenomic databases

eggNOGi COG5260.
GeneTreei ENSGT00550000074490.
HOVERGENi HBG079670.
InParanoidi Q3MHT4.
OMAi REALGCH.
OrthoDBi EOG7353WD.
PhylomeDBi Q3MHT4.
TreeFami TF354308.

Miscellaneous databases

NextBioi 702524.
PROi Q3MHT4.

Gene expression databases

Genevestigatori Q3MHT4.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR015880. Znf_C2H2-like.
[Graphical view ]
Pfami PF03828. PAP_assoc. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiSTPAP_RAT
AccessioniPrimary (citable) accession number: Q3MHT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 25, 2005
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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