ID   UB2L3_BOVIN             Reviewed;         154 AA.
AC   Q3MHP1; Q1JPH3;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   16-JUN-2009, entry version 33.
DE   RecName: Full=Ubiquitin-conjugating enzyme E2 L3;
DE            EC=6.3.2.19;
DE   AltName: Full=Ubiquitin-protein ligase L3;
DE   AltName: Full=Ubiquitin carrier protein L3;
GN   Name=UBE2L3;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-154.
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
CC   -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC       proteins. Mediates the selective degradation of short-lived and
CC       abnormal proteins. Functions in the E6/E6-AP-induced
CC       ubiquitination of p53/TP53 (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP +
CC       diphosphate + protein N-ubiquityllysine.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Binds UBE3A, HEI10, CBL, ZAP70, RNF19A, RNF19B and
CC       RNF144B (By similarity).
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
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DR   EMBL; BC105164; AAI05165.1; -; mRNA.
DR   EMBL; BT025380; ABF57336.1; -; mRNA.
DR   IPI; IPI00711977; -.
DR   RefSeq; NP_001071562.1; -.
DR   UniGene; Bt.34832; -.
DR   PRIDE; Q3MHP1; -.
DR   Ensembl; ENSBTAG00000013038; Bos taurus.
DR   GeneID; 767894; -.
DR   KEGG; bta:767894; -.
DR   HOVERGEN; Q3MHP1; -.
DR   BRENDA; 6.3.2.19; 251.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004842; F:ubiquitin-protein ligase activity; IEA:EC.
DR   GO; GO:0019941; P:modification-dependent protein catabolic pr...; IEA:UniProtKB-KW.
DR   GO; GO:0043687; P:post-translational protein modification; IEA:InterPro.
DR   GO; GO:0051246; P:regulation of protein metabolic process; IEA:InterPro.
DR   InterPro; IPR016135; UBQ-conjugat/RWD-like.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   Gene3D; G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.
DR   Pfam; PF00179; UQ_con; 1.
DR   ProDom; PD000461; UBQ_conjugat; 1.
DR   SMART; SM00212; UBCc; 1.
DR   PROSITE; PS00183; UBIQUITIN_CONJUGAT_1; 1.
DR   PROSITE; PS50127; UBIQUITIN_CONJUGAT_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Ligase; Nucleotide-binding; Ubl conjugation pathway.
FT   CHAIN         1    154       Ubiquitin-conjugating enzyme E2 L3.
FT                                /FTId=PRO_0000245037.
FT   ACT_SITE     86     86       Glycyl thioester intermediate (By
FT                                similarity).
SQ   SEQUENCE   154 AA;  17862 MW;  F5A30243BE3C9985 CRC64;
     MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE
     YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP
     LRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVD
//