Q3MHP1 (UB2L3_BOVIN) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 64.
History...
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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ubiquitin-conjugating enzyme E2 L3 EC=6.3.2.19 Alternative name(s): Ubiquitin carrier protein L3 Ubiquitin-protein ligase L3 | ||
| Gene names |
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| Organism | Bos taurus (Bovine) [Reference proteome] | ||
| Taxonomic identifier | 9913 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 154 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis By similarity. |
| Catalytic activity | ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. |
| Pathway | |
| Subunit structure | Interacts with PARK2; involved in ubiquitination and degradation of misfolded proteins. Interacts with UBE3A. Interacts with CCNB1IP1, CBL, ZAP70, RNF19A, RNF19B and RNF144B. Interacts with ARIH1. Interacts with ARIH2 (via RING-type 1). Interacts with NCOA1; they functionally interact to regulate progesterone receptor transcriptional activity By similarity. |
| Subcellular location | |
| Domain | In contrast to other ubiquitin-conjugating enzymes E2, residues essential for lysine reactivity are absent: Pro and a His residues are present instead of an Asp and an Asp residues in positions 88 and 119,respectively By similarity. |
| Post-translational modification | Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation By similarity. |
| Sequence similarities | Belongs to the ubiquitin-conjugating enzyme family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 154 | 154 | Ubiquitin-conjugating enzyme E2 L3 | PRO_0000245037 | |||||
Sites | |||||||||
| Active site | 86 | 1 | Glycyl thioester intermediate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 9 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 131 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 138 | 1 | N6-acetyllysine By similarity | ||||||
Sequences
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References
| [1] | NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver. |
| [2] | "Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-154. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BC105164 mRNA. Translation: AAI05165.1. BT025380 mRNA. Translation: ABF57336.1. |
| IPI | IPI00711977. |
| RefSeq | NP_001071562.1. NM_001078094.1. |
| UniGene | Bt.34832. |
3D structure databases | |
| ProteinModelPortal | Q3MHP1. |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | Q3MHP1. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 767894. |
| KEGG | bta:767894. |
Organism-specific databases | |
| CTD | 7332. |
Phylogenomic databases | |
| eggNOG | COG5078. |
| HOGENOM | HOG000233455. |
| HOVERGEN | HBG063308. |
| InParanoid | Q3MHP1. |
| KO | K04552. |
| OrthoDB | EOG498V24. |
Enzyme and pathway databases | |
| BioCyc | CATTLE:ENSBTAG00000013038-MONOMER. |
| UniPathway | UPA00143. |
Family and domain databases | |
| Gene3D | 3.10.110.10. 1 hit. |
| InterPro | IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view] |
| Pfam | PF00179. UQ_con. 1 hit. [Graphical view] |
| SUPFAM | SSF54495. UBQ-conjugat/RWD-like. 1 hit. |
| PROSITE | PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 20918251. |
Entry information
| Entry name | UB2L3_BOVIN | ||||||||
| Accession | Primary (citable) accession number: Q3MHP1 Secondary accession number(s): Q1JPH3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |