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Q3MHN7

- NEIL3_BOVIN

UniProt

Q3MHN7 - NEIL3_BOVIN

Protein

Endonuclease 8-like 3

Gene

NEIL3

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 2 (16 Oct 2013)
      Previous versions | rss
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    Functioni

    DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair By similarity.By similarity

    Catalytic activityi

    Removes damaged bases from DNA, leaving an abasic site.
    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Schiff-base intermediate with DNA; via amino nitrogenPROSITE-ProRule annotation
    Sitei2 – 21Important for monofunctional glycosylase activityBy similarity
    Sitei83 – 831Required for glycosylase activityBy similarity
    Binding sitei194 – 1941DNABy similarity
    Binding sitei273 – 2731DNABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri249 – 28335FPG-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri319 – 34830RanBP2-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. bubble DNA binding Source: UniProtKB
    2. damaged DNA binding Source: InterPro
    3. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB
    4. DNA N-glycosylase activity Source: UniProtKB
    5. double-stranded DNA binding Source: UniProtKB
    6. single-stranded DNA binding Source: UniProtKB
    7. zinc ion binding Source: InterPro

    GO - Biological processi

    1. base-excision repair Source: UniProtKB
    2. DNA catabolic process, endonucleolytic Source: GOC
    3. nucleotide-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease 8-like 3 (EC:3.2.2.-, EC:4.2.99.18)
    Alternative name(s):
    DNA glycosylase/AP lyase Neil3
    Endonuclease VIII-like 3
    Nei-like protein 3
    Gene namesi
    Name:NEIL3
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 27

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 606606Endonuclease 8-like 3PRO_0000248634Add
    BLAST

    Proteomic databases

    PRIDEiQ3MHN7.

    Interactioni

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000007661.

    Family & Domainsi

    Domaini

    The N-terminal region (2-283) contains the glycosylase and lyase activities.By similarity

    Sequence similaritiesi

    Belongs to the FPG family.PROSITE-ProRule annotation
    Contains 1 FPG-type zinc finger.PROSITE-ProRule annotation
    Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri249 – 28335FPG-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri319 – 34830RanBP2-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0266.
    GeneTreeiENSGT00730000110955.
    HOGENOMiHOG000113754.
    HOVERGENiHBG052594.
    InParanoidiQ3MHN7.
    KOiK10569.
    OMAiICFFDSS.
    OrthoDBiEOG7W153D.
    TreeFamiTF331502.

    Family and domain databases

    InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    IPR010666. Znf_GRF.
    IPR001876. Znf_RanBP2.
    [Graphical view]
    PfamiPF06831. H2TH. 1 hit.
    PF06839. zf-GRF. 2 hits.
    PF00641. zf-RanBP. 1 hit.
    [Graphical view]
    SMARTiSM00547. ZnF_RBZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEiPS51068. FPG_CAT. 1 hit.
    PS01242. ZF_FPG_1. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    PS01358. ZF_RANBP2_1. 1 hit.
    PS50199. ZF_RANBP2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3MHN7-1 [UniParc]FASTAAdd to Basket

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    MVEGPGCTLN GEKIRARVRP GQAVTDVRGR ALQGLGGPGS PPAAPGPMGT    50
    SQAAALNNNK NSSQDFLRLF NGHGYSGVET LGKELFMYFG PKALRIHFGM 100
    KGSLVINPLE SKNKNGVSPV FEVQLTKDLI CFFDSSVEIR NSTESQQRIR 150
    VMEELDVCSP RFSFSRAESE VKKQKGRMLC DVLMDQKVLP GVGNIIKNEA 200
    LFDSGFHPSV KVCQLTDEQI HHLVKMIRNF SILFYRCCKV GSALSKHYKV 250
    YKRPNCGQCC CKITVCRLGE NNRMTYFCPH CQKENPQHVD IRMLPVRNTT 300
    VNWPSSRERH LMDCVAQKSE EQWTCEVCTL INKLSSKTCD ACLTSRPADS 350
    VLRNEGNPIV FNNLMKYPCN SFGKSKAKVK INRKTAFGTT TLVLTDFSNK 400
    HSALEREESH SHIPDGEFPS PPPNVCGSDT LNTSKERTNC RSQPSDKVNI 450
    SPVVCSQYKL FSPAHKKLKT THYSSPDLKS CNPGFSNSEL QSSMTDGPCL 500
    LNAGSPRCSK HGRPCALRVV RKSGENKGRH FYACPLAREA QCGFFEWADL 550
    SFPFCNHGKR SIMRTVLKIG PNNGKNFFVC PLGKEKQCNF FQWAQNGPGI 600
    NIIPGC 606
    Length:606
    Mass (Da):67,384
    Last modified:October 16, 2013 - v2
    Checksum:i30EE4917DC869250
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti64 – 641Q → H in AAI05169. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DAAA02060028 Genomic DNA. No translation available.
    BC105168 mRNA. Translation: AAI05169.1.
    RefSeqiNP_001029662.1. NM_001034490.2.
    UniGeneiBt.37835.

    Genome annotation databases

    EnsembliENSBTAT00000007661; ENSBTAP00000007661; ENSBTAG00000005825.
    GeneIDi515343.
    KEGGibta:515343.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DAAA02060028 Genomic DNA. No translation available.
    BC105168 mRNA. Translation: AAI05169.1 .
    RefSeqi NP_001029662.1. NM_001034490.2.
    UniGenei Bt.37835.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000007661.

    Proteomic databases

    PRIDEi Q3MHN7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000007661 ; ENSBTAP00000007661 ; ENSBTAG00000005825 .
    GeneIDi 515343.
    KEGGi bta:515343.

    Organism-specific databases

    CTDi 55247.

    Phylogenomic databases

    eggNOGi COG0266.
    GeneTreei ENSGT00730000110955.
    HOGENOMi HOG000113754.
    HOVERGENi HBG052594.
    InParanoidi Q3MHN7.
    KOi K10569.
    OMAi ICFFDSS.
    OrthoDBi EOG7W153D.
    TreeFami TF331502.

    Miscellaneous databases

    NextBioi 20871781.

    Family and domain databases

    InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    IPR010666. Znf_GRF.
    IPR001876. Znf_RanBP2.
    [Graphical view ]
    Pfami PF06831. H2TH. 1 hit.
    PF06839. zf-GRF. 2 hits.
    PF00641. zf-RanBP. 1 hit.
    [Graphical view ]
    SMARTi SM00547. ZnF_RBZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEi PS51068. FPG_CAT. 1 hit.
    PS01242. ZF_FPG_1. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    PS01358. ZF_RANBP2_1. 1 hit.
    PS50199. ZF_RANBP2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hereford.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Liver.

    Entry informationi

    Entry nameiNEIL3_BOVIN
    AccessioniPrimary (citable) accession number: Q3MHN7
    Secondary accession number(s): F1N383
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: October 16, 2013
    Last modified: October 1, 2014
    This is version 62 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3