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Q3MHN7 (NEIL3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endonuclease 8-like 3

EC=3.2.2.-
EC=4.2.99.18
Alternative name(s):
DNA glycosylase/AP lyase Neil3
Endonuclease VIII-like 3
Nei-like protein 3
Gene names
Name:NEIL3
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length606 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair By similarity.

Catalytic activity

Removes damaged bases from DNA, leaving an abasic site.

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Subcellular location

Nucleus By similarity.

Domain

The N-terminal region (2-283) contains the glycosylase and lyase activities By similarity.

Sequence similarities

Belongs to the FPG family.

Contains 1 FPG-type zinc finger.

Contains 1 RanBP2-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 606606Endonuclease 8-like 3
PRO_0000248634

Regions

Zinc finger249 – 28335FPG-type
Zinc finger319 – 34830RanBP2-type

Sites

Active site21Schiff-base intermediate with DNA; via amino nitrogen By similarity
Binding site1941DNA By similarity
Binding site2731DNA By similarity
Site21Important for monofunctional glycosylase activity By similarity
Site831Required for glycosylase activity By similarity

Experimental info

Sequence conflict641Q → H in AAI05169. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q3MHN7 [UniParc].

Last modified October 16, 2013. Version 2.
Checksum: 30EE4917DC869250

FASTA60667,384
        10         20         30         40         50         60 
MVEGPGCTLN GEKIRARVRP GQAVTDVRGR ALQGLGGPGS PPAAPGPMGT SQAAALNNNK 

        70         80         90        100        110        120 
NSSQDFLRLF NGHGYSGVET LGKELFMYFG PKALRIHFGM KGSLVINPLE SKNKNGVSPV 

       130        140        150        160        170        180 
FEVQLTKDLI CFFDSSVEIR NSTESQQRIR VMEELDVCSP RFSFSRAESE VKKQKGRMLC 

       190        200        210        220        230        240 
DVLMDQKVLP GVGNIIKNEA LFDSGFHPSV KVCQLTDEQI HHLVKMIRNF SILFYRCCKV 

       250        260        270        280        290        300 
GSALSKHYKV YKRPNCGQCC CKITVCRLGE NNRMTYFCPH CQKENPQHVD IRMLPVRNTT 

       310        320        330        340        350        360 
VNWPSSRERH LMDCVAQKSE EQWTCEVCTL INKLSSKTCD ACLTSRPADS VLRNEGNPIV 

       370        380        390        400        410        420 
FNNLMKYPCN SFGKSKAKVK INRKTAFGTT TLVLTDFSNK HSALEREESH SHIPDGEFPS 

       430        440        450        460        470        480 
PPPNVCGSDT LNTSKERTNC RSQPSDKVNI SPVVCSQYKL FSPAHKKLKT THYSSPDLKS 

       490        500        510        520        530        540 
CNPGFSNSEL QSSMTDGPCL LNAGSPRCSK HGRPCALRVV RKSGENKGRH FYACPLAREA 

       550        560        570        580        590        600 
QCGFFEWADL SFPFCNHGKR SIMRTVLKIG PNNGKNFFVC PLGKEKQCNF FQWAQNGPGI 


NIIPGC 

« Hide

References

[1]"A whole-genome assembly of the domestic cow, Bos taurus."
Zimin A.V., Delcher A.L., Florea L., Kelley D.R., Schatz M.C., Puiu D., Hanrahan F., Pertea G., Van Tassell C.P., Sonstegard T.S., Marcais G., Roberts M., Subramanian P., Yorke J.A., Salzberg S.L.
Genome Biol. 10:R42.01-R42.10(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hereford.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DAAA02060028 Genomic DNA. No translation available.
BC105168 mRNA. Translation: AAI05169.1.
RefSeqNP_001029662.1. NM_001034490.2.
UniGeneBt.37835.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000007661.

Proteomic databases

PRIDEQ3MHN7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000007661; ENSBTAP00000007661; ENSBTAG00000005825.
GeneID515343.
KEGGbta:515343.

Organism-specific databases

CTD55247.

Phylogenomic databases

eggNOGCOG0266.
GeneTreeENSGT00730000110955.
HOGENOMHOG000113754.
HOVERGENHBG052594.
InParanoidQ3MHN7.
KOK10569.
OMAICFFDSS.
OrthoDBEOG7W153D.
TreeFamTF331502.

Family and domain databases

InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010666. Znf_GRF.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamPF06831. H2TH. 1 hit.
PF06839. zf-GRF. 2 hits.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20871781.

Entry information

Entry nameNEIL3_BOVIN
AccessionPrimary (citable) accession number: Q3MHN7
Secondary accession number(s): F1N383
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 16, 2013
Last modified: July 9, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families