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Q3MHN7

- NEIL3_BOVIN

UniProt

Q3MHN7 - NEIL3_BOVIN

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Protein
Endonuclease 8-like 3
Gene
NEIL3
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair By similarity.

Catalytic activityi

Removes damaged bases from DNA, leaving an abasic site.
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNA; via amino nitrogen By similarity
Sitei2 – 21Important for monofunctional glycosylase activity By similarity
Sitei83 – 831Required for glycosylase activity By similarity
Binding sitei194 – 1941DNA By similarity
Binding sitei273 – 2731DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri249 – 28335FPG-type
Add
BLAST
Zinc fingeri319 – 34830RanBP2-type
Add
BLAST

GO - Molecular functioni

  1. DNA N-glycosylase activity Source: UniProtKB
  2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB
  3. bubble DNA binding Source: UniProtKB
  4. damaged DNA binding Source: InterPro
  5. double-stranded DNA binding Source: UniProtKB
  6. single-stranded DNA binding Source: UniProtKB
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA catabolic process, endonucleolytic Source: GOC
  2. base-excision repair Source: UniProtKB
  3. nucleotide-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8-like 3 (EC:3.2.2.-, EC:4.2.99.18)
Alternative name(s):
DNA glycosylase/AP lyase Neil3
Endonuclease VIII-like 3
Nei-like protein 3
Gene namesi
Name:NEIL3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 27

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606Endonuclease 8-like 3
PRO_0000248634Add
BLAST

Proteomic databases

PRIDEiQ3MHN7.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007661.

Family & Domainsi

Domaini

The N-terminal region (2-283) contains the glycosylase and lyase activities By similarity.

Sequence similaritiesi

Belongs to the FPG family.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG0266.
GeneTreeiENSGT00730000110955.
HOGENOMiHOG000113754.
HOVERGENiHBG052594.
InParanoidiQ3MHN7.
KOiK10569.
OMAiICFFDSS.
OrthoDBiEOG7W153D.
TreeFamiTF331502.

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010666. Znf_GRF.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF06831. H2TH. 1 hit.
PF06839. zf-GRF. 2 hits.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3MHN7-1 [UniParc]FASTAAdd to Basket

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MVEGPGCTLN GEKIRARVRP GQAVTDVRGR ALQGLGGPGS PPAAPGPMGT    50
SQAAALNNNK NSSQDFLRLF NGHGYSGVET LGKELFMYFG PKALRIHFGM 100
KGSLVINPLE SKNKNGVSPV FEVQLTKDLI CFFDSSVEIR NSTESQQRIR 150
VMEELDVCSP RFSFSRAESE VKKQKGRMLC DVLMDQKVLP GVGNIIKNEA 200
LFDSGFHPSV KVCQLTDEQI HHLVKMIRNF SILFYRCCKV GSALSKHYKV 250
YKRPNCGQCC CKITVCRLGE NNRMTYFCPH CQKENPQHVD IRMLPVRNTT 300
VNWPSSRERH LMDCVAQKSE EQWTCEVCTL INKLSSKTCD ACLTSRPADS 350
VLRNEGNPIV FNNLMKYPCN SFGKSKAKVK INRKTAFGTT TLVLTDFSNK 400
HSALEREESH SHIPDGEFPS PPPNVCGSDT LNTSKERTNC RSQPSDKVNI 450
SPVVCSQYKL FSPAHKKLKT THYSSPDLKS CNPGFSNSEL QSSMTDGPCL 500
LNAGSPRCSK HGRPCALRVV RKSGENKGRH FYACPLAREA QCGFFEWADL 550
SFPFCNHGKR SIMRTVLKIG PNNGKNFFVC PLGKEKQCNF FQWAQNGPGI 600
NIIPGC 606
Length:606
Mass (Da):67,384
Last modified:October 16, 2013 - v2
Checksum:i30EE4917DC869250
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641Q → H in AAI05169. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DAAA02060028 Genomic DNA. No translation available.
BC105168 mRNA. Translation: AAI05169.1.
RefSeqiNP_001029662.1. NM_001034490.2.
UniGeneiBt.37835.

Genome annotation databases

EnsembliENSBTAT00000007661; ENSBTAP00000007661; ENSBTAG00000005825.
GeneIDi515343.
KEGGibta:515343.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DAAA02060028 Genomic DNA. No translation available.
BC105168 mRNA. Translation: AAI05169.1 .
RefSeqi NP_001029662.1. NM_001034490.2.
UniGenei Bt.37835.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000007661.

Proteomic databases

PRIDEi Q3MHN7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000007661 ; ENSBTAP00000007661 ; ENSBTAG00000005825 .
GeneIDi 515343.
KEGGi bta:515343.

Organism-specific databases

CTDi 55247.

Phylogenomic databases

eggNOGi COG0266.
GeneTreei ENSGT00730000110955.
HOGENOMi HOG000113754.
HOVERGENi HBG052594.
InParanoidi Q3MHN7.
KOi K10569.
OMAi ICFFDSS.
OrthoDBi EOG7W153D.
TreeFami TF331502.

Miscellaneous databases

NextBioi 20871781.

Family and domain databases

InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010666. Znf_GRF.
IPR001876. Znf_RanBP2.
[Graphical view ]
Pfami PF06831. H2TH. 1 hit.
PF06839. zf-GRF. 2 hits.
PF00641. zf-RanBP. 1 hit.
[Graphical view ]
SMARTi SM00547. ZnF_RBZ. 1 hit.
[Graphical view ]
SUPFAMi SSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEi PS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hereford.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiNEIL3_BOVIN
AccessioniPrimary (citable) accession number: Q3MHN7
Secondary accession number(s): F1N383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 16, 2013
Last modified: July 9, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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