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Q3MHN7 (NEIL3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Endonuclease 8-like 3
EC=3.2.2.-
EC=4.2.99.18
Alternative name(s):
DNA glycosylase/AP lyase Neil3
Endonuclease VIII-like 3
Nei-like protein 3
Gene names
Name:NEIL3
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length606 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as the active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and the human ortholog has been shown to possess AP lyase activity. Prefers single-stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected By similarity.

Catalytic activity

Removes damaged bases from DNA, leaving an abasic site.

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the FPG family.

Contains 1 FPG-type zinc finger.

Contains 1 RanBP2-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 606605Endonuclease 8-like 3
PRO_0000248634

Regions

Zinc finger249 – 28335FPG-type
Zinc finger319 – 34830RanBP2-type

Sites

Active site21Schiff-base intermediate with DNA; via amino nitrogen By similarity
Binding site1941DNA By similarity
Binding site2731DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3MHN7 [UniParc].

Last modified October 25, 2005. Version 1.
Checksum: 483D7727DC869607

FASTA60667,393
        10         20         30         40         50         60 
MVEGPGCTLN GEKIRARVRP GQAVTDVRGR ALQGLGGPGS PPAAPGPMGT SQAAALNNNK 

        70         80         90        100        110        120 
NSSHDFLRLF NGHGYSGVET LGKELFMYFG PKALRIHFGM KGSLVINPLE SKNKNGVSPV 

       130        140        150        160        170        180 
FEVQLTKDLI CFFDSSVEIR NSTESQQRIR VMEELDVCSP RFSFSRAESE VKKQKGRMLC 

       190        200        210        220        230        240 
DVLMDQKVLP GVGNIIKNEA LFDSGFHPSV KVCQLTDEQI HHLVKMIRNF SILFYRCCKV 

       250        260        270        280        290        300 
GSALSKHYKV YKRPNCGQCC CKITVCRLGE NNRMTYFCPH CQKENPQHVD IRMLPVRNTT 

       310        320        330        340        350        360 
VNWPSSRERH LMDCVAQKSE EQWTCEVCTL INKLSSKTCD ACLTSRPADS VLRNEGNPIV 

       370        380        390        400        410        420 
FNNLMKYPCN SFGKSKAKVK INRKTAFGTT TLVLTDFSNK HSALEREESH SHIPDGEFPS 

       430        440        450        460        470        480 
PPPNVCGSDT LNTSKERTNC RSQPSDKVNI SPVVCSQYKL FSPAHKKLKT THYSSPDLKS 

       490        500        510        520        530        540 
CNPGFSNSEL QSSMTDGPCL LNAGSPRCSK HGRPCALRVV RKSGENKGRH FYACPLAREA 

       550        560        570        580        590        600 
QCGFFEWADL SFPFCNHGKR SIMRTVLKIG PNNGKNFFVC PLGKEKQCNF FQWAQNGPGI 


NIIPGC

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC105168 mRNA. Translation: AAI05169.1.
IPIIPI00706576.
RefSeqNP_001029662.1. NM_001034490.2.
UniGeneBt.37835.

3D structure databases

ProteinModelPortalQ3MHN7.
ModBaseSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000007661.

Proteomic databases

PRIDEQ3MHN7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID515343.
KEGGbta:515343.

Organism-specific databases

CTD55247.

Phylogenomic databases

eggNOGCOG0266.
HOGENOMHOG000113754.
HOVERGENHBG052594.
InParanoidQ3MHN7.
KOK10569.
OrthoDBEOG48GW30.

Enzyme and pathway databases

BioCycCATTLE:515343-MONOMER.

Family and domain databases

InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010666. Znf_GRF.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamPF06831. H2TH. 1 hit.
PF06839. zf-GRF. 2 hits.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMSSF81624. Form_DNAglyc_cat. 1 hit.
SSF46946. Ribosomal_H2TH. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20871781.

Entry information

Entry nameNEIL3_BOVIN
AccessionPrimary (citable) accession number: Q3MHN7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 25, 2005
Last modified: May 29, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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