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Protein

Endonuclease 8-like 3

Gene

NEIL3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair (By similarity).By similarity

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Schiff-base intermediate with DNA; via amino nitrogenPROSITE-ProRule annotation1
Sitei2Important for monofunctional glycosylase activityBy similarity1
Sitei83Required for glycosylase activityBy similarity1
Binding sitei194DNABy similarity1
Binding sitei273DNABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri249 – 283FPG-typePROSITE-ProRule annotationAdd BLAST35
Zinc fingeri319 – 348RanBP2-typePROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Glycosidase, Hydrolase, Lyase, Multifunctional enzyme
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8-like 3 (EC:3.2.2.-, EC:4.2.99.18)
Alternative name(s):
DNA glycosylase/AP lyase Neil3
Endonuclease VIII-like 3
Nei-like protein 3
Gene namesi
Name:NEIL3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 27

Organism-specific databases

VGNCiVGNC:31990 NEIL3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002486341 – 606Endonuclease 8-like 3Add BLAST606

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei451PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3MHN7
PRIDEiQ3MHN7

Expressioni

Gene expression databases

BgeeiENSBTAG00000005825

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007661

Structurei

3D structure databases

SMRiQ3MHN7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region (2-283) contains the glycosylase and lyase activities.By similarity

Sequence similaritiesi

Belongs to the FPG family.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri249 – 283FPG-typePROSITE-ProRule annotationAdd BLAST35
Zinc fingeri319 – 348RanBP2-typePROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IETI Eukaryota
ENOG410ZE81 LUCA
GeneTreeiENSGT00730000110955
HOGENOMiHOG000113754
HOVERGENiHBG052594
InParanoidiQ3MHN7
KOiK10569
OMAiIHFGMKG
OrthoDBiEOG091G038I
TreeFamiTF331502

Family and domain databases

Gene3Di3.20.190.10, 1 hit
InterProiView protein in InterPro
IPR015886 DNA_glyclase/AP_lyase_DNA-bd
IPR015887 DNA_glyclase_Znf_dom_DNA_BS
IPR012319 FPG_cat
IPR035937 MutM-like_N-ter
IPR010979 Ribosomal_S13-like_H2TH
IPR000214 Znf_DNA_glyclase/AP_lyase
IPR010666 Znf_GRF
IPR001876 Znf_RanBP2
IPR036443 Znf_RanBP2_sf
PfamiView protein in Pfam
PF06831 H2TH, 1 hit
PF06839 zf-GRF, 2 hits
PF00641 zf-RanBP, 1 hit
SMARTiView protein in SMART
SM01232 H2TH, 1 hit
SM00547 ZnF_RBZ, 1 hit
SUPFAMiSSF46946 SSF46946, 1 hit
SSF81624 SSF81624, 1 hit
SSF90209 SSF90209, 1 hit
PROSITEiView protein in PROSITE
PS51068 FPG_CAT, 1 hit
PS01242 ZF_FPG_1, 1 hit
PS51066 ZF_FPG_2, 1 hit
PS01358 ZF_RANBP2_1, 1 hit
PS50199 ZF_RANBP2_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q3MHN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEGPGCTLN GEKIRARVRP GQAVTDVRGR ALQGLGGPGS PPAAPGPMGT
60 70 80 90 100
SQAAALNNNK NSSQDFLRLF NGHGYSGVET LGKELFMYFG PKALRIHFGM
110 120 130 140 150
KGSLVINPLE SKNKNGVSPV FEVQLTKDLI CFFDSSVEIR NSTESQQRIR
160 170 180 190 200
VMEELDVCSP RFSFSRAESE VKKQKGRMLC DVLMDQKVLP GVGNIIKNEA
210 220 230 240 250
LFDSGFHPSV KVCQLTDEQI HHLVKMIRNF SILFYRCCKV GSALSKHYKV
260 270 280 290 300
YKRPNCGQCC CKITVCRLGE NNRMTYFCPH CQKENPQHVD IRMLPVRNTT
310 320 330 340 350
VNWPSSRERH LMDCVAQKSE EQWTCEVCTL INKLSSKTCD ACLTSRPADS
360 370 380 390 400
VLRNEGNPIV FNNLMKYPCN SFGKSKAKVK INRKTAFGTT TLVLTDFSNK
410 420 430 440 450
HSALEREESH SHIPDGEFPS PPPNVCGSDT LNTSKERTNC RSQPSDKVNI
460 470 480 490 500
SPVVCSQYKL FSPAHKKLKT THYSSPDLKS CNPGFSNSEL QSSMTDGPCL
510 520 530 540 550
LNAGSPRCSK HGRPCALRVV RKSGENKGRH FYACPLAREA QCGFFEWADL
560 570 580 590 600
SFPFCNHGKR SIMRTVLKIG PNNGKNFFVC PLGKEKQCNF FQWAQNGPGI

NIIPGC
Length:606
Mass (Da):67,384
Last modified:October 16, 2013 - v2
Checksum:i30EE4917DC869250
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64Q → H in AAI05169 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02060028 Genomic DNA No translation available.
BC105168 mRNA Translation: AAI05169.1
RefSeqiNP_001029662.1, NM_001034490.2
UniGeneiBt.37835

Genome annotation databases

EnsembliENSBTAT00000007661; ENSBTAP00000007661; ENSBTAG00000005825
GeneIDi515343
KEGGibta:515343

Similar proteinsi

Entry informationi

Entry nameiNEIL3_BOVIN
AccessioniPrimary (citable) accession number: Q3MHN7
Secondary accession number(s): F1N383
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 16, 2013
Last modified: May 23, 2018
This is version 80 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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