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Protein

Endonuclease 8-like 3

Gene

NEIL3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair (By similarity).By similarity

Catalytic activityi

Removes damaged bases from DNA, leaving an abasic site.
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNA; via amino nitrogenPROSITE-ProRule annotation
Sitei2 – 21Important for monofunctional glycosylase activityBy similarity
Sitei83 – 831Required for glycosylase activityBy similarity
Binding sitei194 – 1941DNABy similarity
Binding sitei273 – 2731DNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri249 – 28335FPG-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri319 – 34830RanBP2-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8-like 3 (EC:3.2.2.-, EC:4.2.99.18)
Alternative name(s):
DNA glycosylase/AP lyase Neil3
Endonuclease VIII-like 3
Nei-like protein 3
Gene namesi
Name:NEIL3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 27

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606Endonuclease 8-like 3PRO_0000248634Add
BLAST

Proteomic databases

PRIDEiQ3MHN7.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007661.

Family & Domainsi

Domaini

The N-terminal region (2-283) contains the glycosylase and lyase activities.By similarity

Sequence similaritiesi

Belongs to the FPG family.PROSITE-ProRule annotation
Contains 1 FPG-type zinc finger.PROSITE-ProRule annotation
Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri249 – 28335FPG-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri319 – 34830RanBP2-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG0266.
GeneTreeiENSGT00730000110955.
HOGENOMiHOG000113754.
HOVERGENiHBG052594.
InParanoidiQ3MHN7.
KOiK10569.
OMAiCFFDSSV.
OrthoDBiEOG7W153D.
TreeFamiTF331502.

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010666. Znf_GRF.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF06831. H2TH. 1 hit.
PF06839. zf-GRF. 2 hits.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3MHN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEGPGCTLN GEKIRARVRP GQAVTDVRGR ALQGLGGPGS PPAAPGPMGT
60 70 80 90 100
SQAAALNNNK NSSQDFLRLF NGHGYSGVET LGKELFMYFG PKALRIHFGM
110 120 130 140 150
KGSLVINPLE SKNKNGVSPV FEVQLTKDLI CFFDSSVEIR NSTESQQRIR
160 170 180 190 200
VMEELDVCSP RFSFSRAESE VKKQKGRMLC DVLMDQKVLP GVGNIIKNEA
210 220 230 240 250
LFDSGFHPSV KVCQLTDEQI HHLVKMIRNF SILFYRCCKV GSALSKHYKV
260 270 280 290 300
YKRPNCGQCC CKITVCRLGE NNRMTYFCPH CQKENPQHVD IRMLPVRNTT
310 320 330 340 350
VNWPSSRERH LMDCVAQKSE EQWTCEVCTL INKLSSKTCD ACLTSRPADS
360 370 380 390 400
VLRNEGNPIV FNNLMKYPCN SFGKSKAKVK INRKTAFGTT TLVLTDFSNK
410 420 430 440 450
HSALEREESH SHIPDGEFPS PPPNVCGSDT LNTSKERTNC RSQPSDKVNI
460 470 480 490 500
SPVVCSQYKL FSPAHKKLKT THYSSPDLKS CNPGFSNSEL QSSMTDGPCL
510 520 530 540 550
LNAGSPRCSK HGRPCALRVV RKSGENKGRH FYACPLAREA QCGFFEWADL
560 570 580 590 600
SFPFCNHGKR SIMRTVLKIG PNNGKNFFVC PLGKEKQCNF FQWAQNGPGI

NIIPGC
Length:606
Mass (Da):67,384
Last modified:October 16, 2013 - v2
Checksum:i30EE4917DC869250
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641Q → H in AAI05169 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02060028 Genomic DNA. No translation available.
BC105168 mRNA. Translation: AAI05169.1.
RefSeqiNP_001029662.1. NM_001034490.2.
UniGeneiBt.37835.

Genome annotation databases

EnsembliENSBTAT00000007661; ENSBTAP00000007661; ENSBTAG00000005825.
GeneIDi515343.
KEGGibta:515343.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02060028 Genomic DNA. No translation available.
BC105168 mRNA. Translation: AAI05169.1.
RefSeqiNP_001029662.1. NM_001034490.2.
UniGeneiBt.37835.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007661.

Proteomic databases

PRIDEiQ3MHN7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007661; ENSBTAP00000007661; ENSBTAG00000005825.
GeneIDi515343.
KEGGibta:515343.

Organism-specific databases

CTDi55247.

Phylogenomic databases

eggNOGiCOG0266.
GeneTreeiENSGT00730000110955.
HOGENOMiHOG000113754.
HOVERGENiHBG052594.
InParanoidiQ3MHN7.
KOiK10569.
OMAiCFFDSSV.
OrthoDBiEOG7W153D.
TreeFamiTF331502.

Miscellaneous databases

NextBioi20871781.

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010666. Znf_GRF.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF06831. H2TH. 1 hit.
PF06839. zf-GRF. 2 hits.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hereford.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiNEIL3_BOVIN
AccessioniPrimary (citable) accession number: Q3MHN7
Secondary accession number(s): F1N383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 16, 2013
Last modified: April 29, 2015
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.