Reviewed, UniProtKB/Swiss-Prot Q3MHL4 (SAHH_BOVIN)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
      Short name=AdoHcyase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase

Gene names

Name:

AHCY

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine By similarity.
Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity. Melanosome By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
PTM	Acetylation
Gene Ontology (GO)
Biological process	S-adenosylhomocysteine catabolic process Inferred from sequence or structural similarity. Source: AgBase one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from sequence or structural similarity. Source: AgBase melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from sequence or structural similarity. Source: AgBase copper ion binding Inferred from sequence or structural similarity. Source: AgBase protein self-association Inferred from sequence or structural similarity. Source: AgBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 432	431	Adenosylhomocysteinase		PRO_0000260217
Regions
	Region	183 – 350	168	NAD binding By similarity
Sites
	Binding site	57	1	Substrate By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity
	Binding site	186	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	408	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MHL4-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 314540823FA21BAB	FASTA	432	47,638
	10 20 30 40 50 60 MSDKLPYKVA DISLAAWGRK ALDLAENEMP GLMHMREMYS ASKPLKGARI AGCLHMTVET 70 80 90 100 110 120 AVLIETLVAL GAEVRWSSCN IFSTQDHAAA AIAKAGIPVY AWKGETDEEY LWCIEQTLYF 130 140 150 160 170 180 KDGPLNMILD DGGDLTNLIH TKYPQLLSGI RGISEETTTG VHNLYKMMAK GILKVPAINV 190 200 210 220 230 240 NDSVTKSKFD NLYGCRESLI DGIKRATDVM IAGKVAVVAG YGDVGKGCAQ ALRGFGARVI 250 260 270 280 290 300 ITEIDPINAL QAAMEGYEVT TMDEACQEGN IFVTTTGCTD IILGQHFEQM KDDAIVCNIG 310 320 330 340 350 360 HFDVEIDVKW LNENAVEKVN IKPQVDRYLL KNGRRIILLA EGRLVNLGCA MGHPSFVMSN 370 380 390 400 410 420 SFTNQVLAQI ELWTHPDKYP VGVHFLPKKL DEAVAEAHLG KLNVKLTKLT EKQAQYLGVS 430 REGPFKPDHY RY

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT030537 mRNA. Translation: ABQ12977.1. BC105194 mRNA. Translation: AAI05195.1.
IPI	IPI00714294.
RefSeq	NP_001029487.1.
UniGene	Bt.12322
3D structure databases
HSSP	HSSP built from PDB template 1K0U based on UniProtKB P10760.
SMR	Q3MHL4. Positions 3-432.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3MHL4.
Genome annotation databases
Ensembl	ENSBTAT00000024092; ENSBTAP00000024092; ENSBTAG00000018101; Bos taurus. [Genome view]
GeneID	508158.
KEGG	bta:508158.
Organism-specific databases
CTD	508158.
Phylogenomic databases
eggNOG	maNOG13409.
HOVERGEN	Q3MHL4.
InParanoid	Q3MHL4.
OMA	VITHDHM.
OrthoDB	EOG9KH5F6.
PhylomeDB	Q3MHL4.
Enzyme and pathway databases
BRENDA	3.3.1.1. 251.
Family and domain databases
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR016040. NAD(P)-bd_dom. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_BOVIN

Accession

Primary (citable) accession number: Q3MHL4
Secondary accession number(s): A5D9B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 44 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)