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Protein

Glutamine--tRNA ligase

Gene

QARS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Glutamine--tRNA ligase. Plays a critical role in brain development.By similarity

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei303L-glutamineBy similarity1
Binding sitei438L-glutamineBy similarity1
Binding sitei457ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi271 – 273ATPBy similarity3
Nucleotide bindingi277 – 283ATPBy similarity7
Nucleotide bindingi486 – 487ATPBy similarity2
Nucleotide bindingi494 – 496ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligase (EC:6.1.1.18By similarity)
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name:
GlnRS
Gene namesi
Name:QARS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002450232 – 775Glutamine--tRNA ligaseAdd BLAST774

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei70PhosphoserineBy similarity1
Modified residuei309N6-acetyllysineBy similarity1
Modified residuei495PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ3MHH4.
PeptideAtlasiQ3MHH4.
PRIDEiQ3MHH4.

Interactioni

Subunit structurei

Monomer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Interacts with RARS. Part of a complex composed of RARS, QARS and AIMP1.By similarity

Protein-protein interaction databases

IntActiQ3MHH4. 2 interactors.
STRINGi9913.ENSBTAP00000025191.

Chemistry databases

BindingDBiQ3MHH4.

Structurei

3D structure databases

ProteinModelPortaliQ3MHH4.
SMRiQ3MHH4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi270 – 280"HIGH" regionAdd BLAST11
Motifi493 – 497"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1148. Eukaryota.
COG0008. LUCA.
HOGENOMiHOG000259233.
HOVERGENiHBG000889.
InParanoidiQ3MHH4.
KOiK01886.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_N.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PF04558. tRNA_synt_1c_R1. 1 hit.
PF04557. tRNA_synt_1c_R2. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3MHH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALDSLSLF TGLGLSEQKA RETLKNTVLS AQLREAATQA QQTLGSSIDK
60 70 80 90 100
ATGTLLYGLA SRLRDPRRLS FLVSYITSRK IHTETQLSAA LEYVRSHPLD
110 120 130 140 150
PINTEDFEQE CGVGVVVTPE QIEEAVEAAI NRHRAKLLVE RYHFSMGLLM
160 170 180 190 200
GEARAALKWA DGKMIKHEVD MQVLHLLGPK TETDLEKKPK VAKARPEETD
210 220 230 240 250
QRTAKDVVEN GEVVVQTLSL MEQLRGEALK FHKPGENYKT PGYVTTPHTM
260 270 280 290 300
DLLKQHLDIT GGQVRTRFPP EPNGILHIGH AKAINFNFGY AKANNGICFL
310 320 330 340 350
RFDDTNPEKE EAKFFTAIYD MVAWLGYTPY KVTYASDYFD QLYAWAVELI
360 370 380 390 400
RRDQAYVCHQ RGEELKGHNP LPSPWRDRPI EESLLLFEAM RKGKFAEGEA
410 420 430 440 450
TLRMKLVMED GKMDPVAYRV KYTPHHRTGD TWCIYPTYDY THCLCDSIEH
460 470 480 490 500
ITHSLCTKEF QARRSSYFWL CNALDVYCPV QWEYGRLNLH YAVVSKRKIL
510 520 530 540 550
QLVAAGAVRD WDDPRLFTLT ALRRRGFPPE AINNFCARVG VTVAQTTMEP
560 570 580 590 600
HLLEACVRDV LNDTAPRAMA VLEPLQVVIT NFPATKALDI QVPNFPADET
610 620 630 640 650
KGFHQVPFGS TVFIERMDFK EEPEPGYKRL AWGQPVGLRH TGYVIELQHV
660 670 680 690 700
VKGPSGCVES LKVTCRRADA GEKPKAFIHW VSQPLTCEIR LYERLFQHKN
710 720 730 740 750
PEDPAEVPGG FLSDLNPASL QVVEAALVDC SVALAKPFDK FQFERLGYFS
760 770
VDPDSNQGQL VFNRTVTLKE DPGKV
Length:775
Mass (Da):87,643
Last modified:October 25, 2005 - v1
Checksum:iC30C9EE302D532F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT025487 mRNA. Translation: ABF57443.1.
BC105237 mRNA. Translation: AAI05238.1.
RefSeqiNP_001029640.1. NM_001034468.2.
UniGeneiBt.7246.

Genome annotation databases

GeneIDi514586.
KEGGibta:514586.

Similar proteinsi

Entry informationi

Entry nameiSYQ_BOVIN
AccessioniPrimary (citable) accession number: Q3MHH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 25, 2005
Last modified: November 22, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families