Reviewed, UniProtKB/Swiss-Prot Q3MHH4 (SYQ_BOVIN)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:

QARS

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	775 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).
Subunit structure	Part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 775	774	Glutaminyl-tRNA synthetase		PRO_0000245023
Regions
	Motif	270 – 280	11	"HIGH" region
	Motif	493 – 497	5	"KMSKS" region
Sites
	Binding site	496	1	ATP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	70	1	Phosphoserine By similarity
	Modified residue	309	1	N6-acetyllysine By similarity
	Modified residue	491	1	Phosphotyrosine By similarity
	Modified residue	620	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MHH4-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: C30C9EE302D532F0	FASTA	775	87,643
	10 20 30 40 50 60 MAALDSLSLF TGLGLSEQKA RETLKNTVLS AQLREAATQA QQTLGSSIDK ATGTLLYGLA 70 80 90 100 110 120 SRLRDPRRLS FLVSYITSRK IHTETQLSAA LEYVRSHPLD PINTEDFEQE CGVGVVVTPE 130 140 150 160 170 180 QIEEAVEAAI NRHRAKLLVE RYHFSMGLLM GEARAALKWA DGKMIKHEVD MQVLHLLGPK 190 200 210 220 230 240 TETDLEKKPK VAKARPEETD QRTAKDVVEN GEVVVQTLSL MEQLRGEALK FHKPGENYKT 250 260 270 280 290 300 PGYVTTPHTM DLLKQHLDIT GGQVRTRFPP EPNGILHIGH AKAINFNFGY AKANNGICFL 310 320 330 340 350 360 RFDDTNPEKE EAKFFTAIYD MVAWLGYTPY KVTYASDYFD QLYAWAVELI RRDQAYVCHQ 370 380 390 400 410 420 RGEELKGHNP LPSPWRDRPI EESLLLFEAM RKGKFAEGEA TLRMKLVMED GKMDPVAYRV 430 440 450 460 470 480 KYTPHHRTGD TWCIYPTYDY THCLCDSIEH ITHSLCTKEF QARRSSYFWL CNALDVYCPV 490 500 510 520 530 540 QWEYGRLNLH YAVVSKRKIL QLVAAGAVRD WDDPRLFTLT ALRRRGFPPE AINNFCARVG 550 560 570 580 590 600 VTVAQTTMEP HLLEACVRDV LNDTAPRAMA VLEPLQVVIT NFPATKALDI QVPNFPADET 610 620 630 640 650 660 KGFHQVPFGS TVFIERMDFK EEPEPGYKRL AWGQPVGLRH TGYVIELQHV VKGPSGCVES 670 680 690 700 710 720 LKVTCRRADA GEKPKAFIHW VSQPLTCEIR LYERLFQHKN PEDPAEVPGG FLSDLNPASL 730 740 750 760 770 QVVEAALVDC SVALAKPFDK FQFERLGYFS VDPDSNQGQL VFNRTVTLKE DPGKV

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Thymus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT025487 mRNA. Translation: ABF57443.1. BC105237 mRNA. Translation: AAI05238.1.
IPI	IPI00708246.
RefSeq	NP_001029640.1.
UniGene	Bt.7246
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3MHH4.
Genome annotation databases
Ensembl	ENSBTAT00000025191; ENSBTAP00000025191; ENSBTAG00000018928; Bos taurus. [Genome view]
GeneID	514586.
KEGG	bta:514586.
Organism-specific databases
CTD	514586.
Phylogenomic databases
HOVERGEN	Q3MHH4.
Enzyme and pathway databases
BRENDA	6.1.1.18. 251.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR007639. Gln-tRNA-synth_Ic_RNA-bd_1. IPR007638. Gln-tRNA-synth_Ic_RNA-bd_2. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. PF04558. tRNA_synt_1c_R1. 1 hit. PF04557. tRNA_synt_1c_R2. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
BindingDB	Q3MHH4.

Entry information

Entry name

SYQ_BOVIN

Accession

Primary (citable) accession number: Q3MHH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	October 25, 2005
Last modified:	October 13, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)