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Protein

Serine palmitoyltransferase 1

Gene

SPTLC1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates. The SPTLC1-SPTLC2-SPTSSB complex displays a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme has the ability to use a broader range of acyl-CoAs (By similarity).By similarity

Catalytic activityi

Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

Cofactori

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid metabolism, Sphingolipid metabolism
LigandPyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-BTA-1660661 Sphingolipid de novo biosynthesis
UniPathwayiUPA00222

Names & Taxonomyi

Protein namesi
Recommended name:
Serine palmitoyltransferase 1 (EC:2.3.1.50)
Alternative name(s):
Long chain base biosynthesis protein 1
Short name:
LCB 1
Serine-palmitoyl-CoA transferase 1
Short name:
SPT 1
Short name:
SPT1
Gene namesi
Name:SPTLC1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Organism-specific databases

VGNCiVGNC:35256 SPTLC1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15LumenalSequence analysisAdd BLAST15
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Topological domaini37 – 473CytoplasmicSequence analysisAdd BLAST437

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002830421 – 473Serine palmitoyltransferase 1Add BLAST473

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei164Phosphotyrosine; by ABLBy similarity1

Post-translational modificationi

Phosphorylation at Tyr-164 inhibits activity and promotes cell survival.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3MHG1
PRIDEiQ3MHG1

Expressioni

Gene expression databases

BgeeiENSBTAG00000002220

Interactioni

Subunit structurei

Heterodimer with SPTLC2 or SPTLC3. Component of the serine palmitoyltransferase (SPT) complex, composed of SPTLC1, either SPTLC2 or SPTLC3, and either SPTSSA or SPTSSB. Interacts with SPTSSA and SPTSSB; the interaction is direct. Interacts with ORMDL3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002872

Structurei

3D structure databases

ProteinModelPortaliQ3MHG1
SMRiQ3MHG1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1358 Eukaryota
COG0156 LUCA
GeneTreeiENSGT00550000074872
HOGENOMiHOG000216602
HOVERGENiHBG003992
InParanoidiQ3MHG1
KOiK00654
OMAiCVGSHFI
OrthoDBiEOG091G0DOG
TreeFamiTF314877

Family and domain databases

Gene3Di3.40.640.101 hit
3.90.1150.102 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit

Sequencei

Sequence statusi: Complete.

Q3MHG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVAEQWVL VEMVQALYEA PAYHLILEGI LILWIIRLLF SKTYKLQERS
60 70 80 90 100
DLTLKEKEEL IEEWQPEPLV PPVSKDHPAL NYNIVSGPPS HNIVVNGKEC
110 120 130 140 150
INFASFNFLG LLDNPRLKAA ALASLKKYGV GTCGPRGFYG TFDVHLDLED
160 170 180 190 200
RLAKFMKTEE AIIYSYGFAT IASAIPAYSK RGDIVFVDKA ACFAIQKGLQ
210 220 230 240 250
ASRSDIKVFN HNDMDDLERL LKEQEIEDQK NPRKARVTRR FIIVEGLYMN
260 270 280 290 300
TGTVCPLPEL VKLKYKYKAR IFLEESLSFG VLGEHGRGVT EHFGISIDDI
310 320 330 340 350
DLISANMENS LASIGGFCCG RSFVIDHQRL SGQGYCFSAS LPPLLAAAAI
360 370 380 390 400
EALNIMEENP GIFAVLKEKC KRIHKALQGI PGLKVVGESI SPALHLQLEE
410 420 430 440 450
TTGCRERDVK LLQEIVTQCM DRGIALTQAR YLEKEEKYLP PPSIRVVVTV
460 470
EQTEEDLEKA ASTISEVAQT VLL
Length:473
Mass (Da):52,788
Last modified:October 25, 2005 - v1
Checksum:i13C8315F8B3572F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105250 mRNA Translation: AAI05251.1
RefSeqiNP_001029921.1, NM_001034749.1
UniGeneiBt.109577

Genome annotation databases

EnsembliENSBTAT00000002872; ENSBTAP00000002872; ENSBTAG00000002220
GeneIDi614165
KEGGibta:614165

Similar proteinsi

Entry informationi

Entry nameiSPTC1_BOVIN
AccessioniPrimary (citable) accession number: Q3MHG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 25, 2005
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome