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Q3MHG1

- SPTC1_BOVIN

UniProt

Q3MHG1 - SPTC1_BOVIN

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Protein

Serine palmitoyltransferase 1

Gene

SPTLC1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates. The SPTLC1-SPTLC2-SPTSSB complex displays a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme has the ability to use a broader range of acyl-CoAs (By similarity).By similarity

Catalytic activityi

Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

Cofactori

Pyridoxal phosphate.By similarity

Pathwayi

GO - Molecular functioni

  1. pyridoxal phosphate binding Source: InterPro
  2. serine C-palmitoyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. ceramide biosynthetic process Source: Ensembl
  2. sphinganine biosynthetic process Source: Ensembl
  3. sphingomyelin biosynthetic process Source: Ensembl
  4. sphingosine biosynthetic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_204257. Sphingolipid de novo biosynthesis.
UniPathwayiUPA00222.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine palmitoyltransferase 1 (EC:2.3.1.50)
Alternative name(s):
Long chain base biosynthesis protein 1
Short name:
LCB 1
Serine-palmitoyl-CoA transferase 1
Short name:
SPT 1
Short name:
SPT1
Gene namesi
Name:SPTLC1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 8

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. SPOTS complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Serine palmitoyltransferase 1PRO_0000283042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei164 – 1641Phosphotyrosine; by ABLBy similarity

Post-translational modificationi

Phosphorylation at Tyr-164 inhibits activity and promotes cell survival.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ3MHG1.

Interactioni

Subunit structurei

Heterodimer with SPTLC2 or SPTLC3. Component of the serine palmitoyltransferase (SPT) complex, composed of SPTLC1, either SPTLC2 or SPTLC3, and either SPTSSA or SPTSSB. Interacts with SPTSSA and SPTSSB; the interaction is direct. Interacts with ORMDL3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002872.

Structurei

3D structure databases

ProteinModelPortaliQ3MHG1.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515LumenalSequence AnalysisAdd
BLAST
Topological domaini37 – 473437CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0156.
GeneTreeiENSGT00550000074872.
HOGENOMiHOG000216602.
HOVERGENiHBG003992.
InParanoidiQ3MHG1.
KOiK00654.
OMAiRVFMDES.
OrthoDBiEOG786H30.
TreeFamiTF314877.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3MHG1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATVAEQWVL VEMVQALYEA PAYHLILEGI LILWIIRLLF SKTYKLQERS
60 70 80 90 100
DLTLKEKEEL IEEWQPEPLV PPVSKDHPAL NYNIVSGPPS HNIVVNGKEC
110 120 130 140 150
INFASFNFLG LLDNPRLKAA ALASLKKYGV GTCGPRGFYG TFDVHLDLED
160 170 180 190 200
RLAKFMKTEE AIIYSYGFAT IASAIPAYSK RGDIVFVDKA ACFAIQKGLQ
210 220 230 240 250
ASRSDIKVFN HNDMDDLERL LKEQEIEDQK NPRKARVTRR FIIVEGLYMN
260 270 280 290 300
TGTVCPLPEL VKLKYKYKAR IFLEESLSFG VLGEHGRGVT EHFGISIDDI
310 320 330 340 350
DLISANMENS LASIGGFCCG RSFVIDHQRL SGQGYCFSAS LPPLLAAAAI
360 370 380 390 400
EALNIMEENP GIFAVLKEKC KRIHKALQGI PGLKVVGESI SPALHLQLEE
410 420 430 440 450
TTGCRERDVK LLQEIVTQCM DRGIALTQAR YLEKEEKYLP PPSIRVVVTV
460 470
EQTEEDLEKA ASTISEVAQT VLL
Length:473
Mass (Da):52,788
Last modified:October 25, 2005 - v1
Checksum:i13C8315F8B3572F0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC105250 mRNA. Translation: AAI05251.1.
RefSeqiNP_001029921.1. NM_001034749.1.
UniGeneiBt.109577.

Genome annotation databases

EnsembliENSBTAT00000002872; ENSBTAP00000002872; ENSBTAG00000002220.
GeneIDi614165.
KEGGibta:614165.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC105250 mRNA. Translation: AAI05251.1 .
RefSeqi NP_001029921.1. NM_001034749.1.
UniGenei Bt.109577.

3D structure databases

ProteinModelPortali Q3MHG1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000002872.

Proteomic databases

PRIDEi Q3MHG1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000002872 ; ENSBTAP00000002872 ; ENSBTAG00000002220 .
GeneIDi 614165.
KEGGi bta:614165.

Organism-specific databases

CTDi 10558.

Phylogenomic databases

eggNOGi COG0156.
GeneTreei ENSGT00550000074872.
HOGENOMi HOG000216602.
HOVERGENi HBG003992.
InParanoidi Q3MHG1.
KOi K00654.
OMAi RVFMDES.
OrthoDBi EOG786H30.
TreeFami TF314877.

Enzyme and pathway databases

UniPathwayi UPA00222 .
Reactomei REACT_204257. Sphingolipid de novo biosynthesis.

Miscellaneous databases

NextBioi 20898974.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiSPTC1_BOVIN
AccessioniPrimary (citable) accession number: Q3MHG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 25, 2005
Last modified: October 1, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3