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Q3MHG1

- SPTC1_BOVIN

UniProt

Q3MHG1 - SPTC1_BOVIN

Protein

Serine palmitoyltransferase 1

Gene

SPTLC1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (25 Oct 2005)
      Previous versions | rss
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    Functioni

    Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates. The SPTLC1-SPTLC2-SPTSSB complex displays a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme has the ability to use a broader range of acyl-CoAs By similarity.By similarity

    Catalytic activityi

    Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: InterPro
    2. serine C-palmitoyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. ceramide biosynthetic process Source: Ensembl
    2. sphinganine biosynthetic process Source: Ensembl
    3. sphingomyelin biosynthetic process Source: Ensembl
    4. sphingosine biosynthetic process Source: Ensembl

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_204257. Sphingolipid de novo biosynthesis.
    UniPathwayiUPA00222.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine palmitoyltransferase 1 (EC:2.3.1.50)
    Alternative name(s):
    Long chain base biosynthesis protein 1
    Short name:
    LCB 1
    Serine-palmitoyl-CoA transferase 1
    Short name:
    SPT 1
    Short name:
    SPT1
    Gene namesi
    Name:SPTLC1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 8

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. SPOTS complex Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 473473Serine palmitoyltransferase 1PRO_0000283042Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei164 – 1641Phosphotyrosine; by ABLBy similarity

    Post-translational modificationi

    Phosphorylation at Tyr-164 inhibits activity and promotes cell survival.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ3MHG1.

    Interactioni

    Subunit structurei

    Heterodimer with SPTLC2 or SPTLC3. Component of the serine palmitoyltransferase (SPT) complex, composed of SPTLC1, either SPTLC2 or SPTLC3, and either SPTSSA or SPTSSB. Interacts with SPTSSA and SPTSSB; the interaction is direct. Interacts with ORMDL3 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000002872.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3MHG1.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515LumenalSequence AnalysisAdd
    BLAST
    Topological domaini37 – 473437CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0156.
    GeneTreeiENSGT00550000074872.
    HOGENOMiHOG000216602.
    HOVERGENiHBG003992.
    InParanoidiQ3MHG1.
    KOiK00654.
    OMAiRVFMDES.
    OrthoDBiEOG786H30.
    TreeFamiTF314877.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q3MHG1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATVAEQWVL VEMVQALYEA PAYHLILEGI LILWIIRLLF SKTYKLQERS    50
    DLTLKEKEEL IEEWQPEPLV PPVSKDHPAL NYNIVSGPPS HNIVVNGKEC 100
    INFASFNFLG LLDNPRLKAA ALASLKKYGV GTCGPRGFYG TFDVHLDLED 150
    RLAKFMKTEE AIIYSYGFAT IASAIPAYSK RGDIVFVDKA ACFAIQKGLQ 200
    ASRSDIKVFN HNDMDDLERL LKEQEIEDQK NPRKARVTRR FIIVEGLYMN 250
    TGTVCPLPEL VKLKYKYKAR IFLEESLSFG VLGEHGRGVT EHFGISIDDI 300
    DLISANMENS LASIGGFCCG RSFVIDHQRL SGQGYCFSAS LPPLLAAAAI 350
    EALNIMEENP GIFAVLKEKC KRIHKALQGI PGLKVVGESI SPALHLQLEE 400
    TTGCRERDVK LLQEIVTQCM DRGIALTQAR YLEKEEKYLP PPSIRVVVTV 450
    EQTEEDLEKA ASTISEVAQT VLL 473
    Length:473
    Mass (Da):52,788
    Last modified:October 25, 2005 - v1
    Checksum:i13C8315F8B3572F0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC105250 mRNA. Translation: AAI05251.1.
    RefSeqiNP_001029921.1. NM_001034749.1.
    UniGeneiBt.109577.

    Genome annotation databases

    EnsembliENSBTAT00000002872; ENSBTAP00000002872; ENSBTAG00000002220.
    GeneIDi614165.
    KEGGibta:614165.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC105250 mRNA. Translation: AAI05251.1 .
    RefSeqi NP_001029921.1. NM_001034749.1.
    UniGenei Bt.109577.

    3D structure databases

    ProteinModelPortali Q3MHG1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000002872.

    Proteomic databases

    PRIDEi Q3MHG1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000002872 ; ENSBTAP00000002872 ; ENSBTAG00000002220 .
    GeneIDi 614165.
    KEGGi bta:614165.

    Organism-specific databases

    CTDi 10558.

    Phylogenomic databases

    eggNOGi COG0156.
    GeneTreei ENSGT00550000074872.
    HOGENOMi HOG000216602.
    HOVERGENi HBG003992.
    InParanoidi Q3MHG1.
    KOi K00654.
    OMAi RVFMDES.
    OrthoDBi EOG786H30.
    TreeFami TF314877.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .
    Reactomei REACT_204257. Sphingolipid de novo biosynthesis.

    Miscellaneous databases

    NextBioi 20898974.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Uterus.

    Entry informationi

    Entry nameiSPTC1_BOVIN
    AccessioniPrimary (citable) accession number: Q3MHG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: October 25, 2005
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3