Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

MTAP

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei18 – 181PhosphateUniRule annotation
Sitei178 – 1781Important for substrate specificityUniRule annotation
Binding sitei196 – 1961Substrate; via amide nitrogenUniRule annotation
Binding sitei197 – 1971PhosphateUniRule annotation
Sitei233 – 2331Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
Name:MTAPUniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

Cytoplasm UniRule annotation. Nucleus UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283S-methyl-5'-thioadenosine phosphorylasePRO_0000415112Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000052827.

Structurei

3D structure databases

ProteinModelPortaliQ3MHF7.
SMRiQ3MHF7. Positions 9-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 612Phosphate bindingUniRule annotation
Regioni93 – 942Phosphate bindingUniRule annotation
Regioni220 – 2223Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228986.
HOVERGENiHBG002487.
InParanoidiQ3MHF7.
KOiK00772.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3MHF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGATPAAV KIGIIGGTGL DDPEILEGRT EKYVDTPFGK PSDALVLGKI
60 70 80 90 100
KNVDCVLLAR HGRQHTIMPS KVNYQANIWA LKEEGCTHVI VTTACGSLKE
110 120 130 140 150
EIQPGDIIII DQFIDRTTRR LQTFYDGNHS CARGVCHIPM AEPFCPKTRE
160 170 180 190 200
VLIETAKKLG LRCHSKGTMI TIEGPRFSSR AESIMFQTWG ADVINMTTVP
210 220 230 240 250
EVVLAKEAGI CYASIAMATD YDCWKEHEEA VSVDRVLKTL KENANKAKSL
260 270 280
LLTTIPQIGS MEWSETLHNM KKMAQFSVLL PRH
Length:283
Mass (Da):31,256
Last modified:October 24, 2005 - v1
Checksum:i9618D53A124CA360
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02022238 Genomic DNA. No translation available.
BC105254 mRNA. Translation: AAI05255.1.
RefSeqiXP_002689552.1. XM_002689506.4.
UniGeneiBt.103308.
Bt.62827.

Genome annotation databases

GeneIDi782907.
KEGGibta:782907.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02022238 Genomic DNA. No translation available.
BC105254 mRNA. Translation: AAI05255.1.
RefSeqiXP_002689552.1. XM_002689506.4.
UniGeneiBt.103308.
Bt.62827.

3D structure databases

ProteinModelPortaliQ3MHF7.
SMRiQ3MHF7. Positions 9-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000052827.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi782907.
KEGGibta:782907.

Organism-specific databases

CTDi4507.

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228986.
HOVERGENiHBG002487.
InParanoidiQ3MHF7.
KOiK00772.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Miscellaneous databases

NextBioi20925764.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hereford.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.
  3. "Physicochemical and immunological studies on mammalian 5'-deoxy-5'-methylthioadenosine phosphorylase."
    Della Ragione F., Oliva A., Gragnaniello V., Russo G.L., Palumbo R., Zappia V.
    J. Biol. Chem. 265:6241-6246(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.

Entry informationi

Entry nameiMTAP_BOVIN
AccessioniPrimary (citable) accession number: Q3MHF7
Secondary accession number(s): F1N1J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2012
Last sequence update: October 24, 2005
Last modified: March 3, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.