Q3MHC1 (NAA60_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-alpha-acetyltransferase 60
EC=2.3.1.48
EC=2.3.1.88

Alternative name(s):
GNAT acetyltransferase
N-acetyltransferase 15
NatF catalytic subunit

Gene names

Name:	Naa60
Synonyms:	Nat15

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase By similarity.
Catalytic activity	Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA. Acetyl-CoA + [histone] = CoA + acetyl-[histone].
Subcellular location	Golgi apparatus membrane By similarity.
Post-translational modification	Acetylated: autoacetylation is required for optimal acetyltransferase activity By similarity.
Sequence similarities	Belongs to the acetyltransferase family. NAA60 subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Chromosome partition
Cellular component	Golgi apparatus Membrane
Molecular function	Acyltransferase Chromatin regulator Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-terminal peptidyl-methionine acetylation Inferred from sequence or structural similarity. Source: UniProtKB cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB chromosome segregation Inferred from sequence or structural similarity. Source: UniProtKB nucleosome assembly Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Golgi membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	H4 histone acetyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB peptide alpha-N-acetyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	N-alpha-acetyltransferase 60		PRO_0000321568
Regions
	Domain	13 – 182	170	N-acetyltransferase
Amino acid modifications
	Modified residue	79	1	N6-acetyllysine; by autocatalysis By similarity
	Modified residue	105	1	N6-acetyllysine; by autocatalysis By similarity
	Modified residue	109	1	N6-acetyllysine; by autocatalysis By similarity
	Modified residue	121	1	N6-acetyllysine; by autocatalysis By similarity
Experimental info
	Sequence conflict	232	1	S → T in AAP83441. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q3MHC1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: F2307DFB07F13F84	FASTA	242	27,493
	10 20 30 40 50 60 MTEVVPSSAL SEVSLRLLCH DDIDTVKHLC GDWFPIEYPD SWYRDITSNK KFFSLAATYR 70 80 90 100 110 120 GAIVGMIVAE IKNRTKIHKE DGDILASSFS VDTQVAYILS LGVVKEFRKH GIGSLLLESL 130 140 150 160 170 180 KDHISTTAQD HCKAIYLHVL TTNNTAINFY ENRDFRQHHY LPYYYSIRGV LKDGFTYVLY 190 200 210 220 230 240 INGGHPPWTI LDYIQHLGSA LANLSPCSIP HRIYRQAHSL LCSFLPWSSI SSKGGIEYSR TM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GNAT family mRNA in the developing rat ovary." Luna F., de la Chesnaye E., Ojeda S.R. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Ovary.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY316589 mRNA. Translation: AAP83441.1. BC105304 mRNA. Translation: AAI05305.1.
IPI	IPI00367756.
RefSeq	NP_001014248.2. NM_001014226.2.
UniGene	Rn.13255. Rn.201091.
3D structure databases
ProteinModelPortal	Q3MHC1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000009936; ENSRNOP00000009936; ENSRNOG00000007280. ENSRNOT00000075238; ENSRNOP00000064770; ENSRNOG00000007280.
GeneID	363545.
KEGG	rno:363545.
UCSC	RGD:1308915. rat.
Organism-specific databases
CTD	79903.
RGD	1308915. Naa60.
Phylogenomic databases
eggNOG	NOG276305.
GeneTree	ENSGT00390000008314.
HOGENOM	HOG000280717.
HOVERGEN	HBG061680.
InParanoid	Q3MHC1.
OrthoDB	EOG45TCNW.
Gene expression databases
Genevestigator	Q3MHC1.
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GNAT_dom. [Graphical view]
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	683635.

Entry information

Entry name

NAA60_RAT

Accession

Primary (citable) accession number: Q3MHC1
Secondary accession number(s): Q7TMZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 25, 2005
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program