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Protein

N-alpha-acetyltransferase 60

Gene

Naa60

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues of the transmembrane proteins, with a strong preference for N-termini facing the cytosol. Displays N-terminal acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase. May also show histone acetyltransferase activity; such results are however unclear in vivo and would require additional experimental evidences.By similarity

Catalytic activityi

Acetyl-CoA + an N-terminal-L-methionyl-[transmembrane protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-[transmembrane protein] + CoA.By similarity
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38SubstrateBy similarity1
Binding sitei81SubstrateBy similarity1
Active sitei97By similarity1
Binding sitei99SubstrateBy similarity1
Active sitei138By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Chromatin regulator, Transferase
Biological processChromosome partition

Names & Taxonomyi

Protein namesi
Recommended name:
N-alpha-acetyltransferase 60 (EC:2.3.1.259By similarity)
Alternative name(s):
Histone acetyltransferase type B protein 4By similarity (EC:2.3.1.48By similarity)
Short name:
HAT4By similarity
N-acetyltransferase 15
N-alpha-acetyltransferase F
Short name:
NatF
Gene namesi
Name:Naa60
Synonyms:Nat15
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1308915. Naa60.

Subcellular locationi

  • Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

  • Note: Probably forms a intramembrane hairpin-like structure in the membrane.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 192CytoplasmicBy similarityAdd BLAST192
Intramembranei193 – 236HelicalBy similarityAdd BLAST44
Topological domaini237 – 242CytoplasmicBy similarity6

GO - Cellular componenti

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003215681 – 242N-alpha-acetyltransferase 60Add BLAST242

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79N6-acetyllysine; by autocatalysisBy similarity1
Modified residuei105N6-acetyllysine; by autocatalysisBy similarity1
Modified residuei109N6-acetyllysine; by autocatalysisBy similarity1
Modified residuei121N6-acetyllysine; by autocatalysisBy similarity1

Post-translational modificationi

Acetylated: autoacetylation is required for optimal acetyltransferase activity.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ3MHC1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000007280.
GenevisibleiQ3MHC1. RN.

Interactioni

Subunit structurei

Monomer and homodimer; monomer in presence of substrate and homodimer in its absence.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009936.

Structurei

3D structure databases

ProteinModelPortaliQ3MHC1.
SMRiQ3MHC1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 182N-acetyltransferasePROSITE-ProRule annotationAdd BLAST170

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 103Acetyl-CoA bindingBy similarity3
Regioni109 – 114Acetyl-CoA bindingBy similarity6
Regioni150 – 153Acetyl-CoA bindingBy similarity4
Regioni162 – 173Required for homodimerizationBy similarityAdd BLAST12

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IQK0. Eukaryota.
ENOG410Y94A. LUCA.
GeneTreeiENSGT00390000008314.
HOGENOMiHOG000280717.
HOVERGENiHBG061680.
InParanoidiQ3MHC1.
KOiK21121.
OMAiSIPQRLY.
OrthoDBiEOG091G0L3L.
PhylomeDBiQ3MHC1.
TreeFamiTF323980.

Family and domain databases

InterProiView protein in InterPro
IPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
PfamiView protein in Pfam
PF00583. Acetyltransf_1. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiView protein in PROSITE
PS51186. GNAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3MHC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEVVPSSAL SEVSLRLLCH DDIDTVKHLC GDWFPIEYPD SWYRDITSNK
60 70 80 90 100
KFFSLAATYR GAIVGMIVAE IKNRTKIHKE DGDILASSFS VDTQVAYILS
110 120 130 140 150
LGVVKEFRKH GIGSLLLESL KDHISTTAQD HCKAIYLHVL TTNNTAINFY
160 170 180 190 200
ENRDFRQHHY LPYYYSIRGV LKDGFTYVLY INGGHPPWTI LDYIQHLGSA
210 220 230 240
LANLSPCSIP HRIYRQAHSL LCSFLPWSSI SSKGGIEYSR TM
Length:242
Mass (Da):27,493
Last modified:October 25, 2005 - v1
Checksum:iF2307DFB07F13F84
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232S → T in AAP83441 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY316589 mRNA. Translation: AAP83441.1.
BC105304 mRNA. Translation: AAI05305.1.
RefSeqiNP_001014248.2. NM_001014226.2.
UniGeneiRn.13255.

Genome annotation databases

EnsembliENSRNOT00000009936; ENSRNOP00000009936; ENSRNOG00000007280.
ENSRNOT00000075238; ENSRNOP00000064770; ENSRNOG00000007280.
GeneIDi363545.
KEGGirno:363545.
UCSCiRGD:1308915. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY316589 mRNA. Translation: AAP83441.1.
BC105304 mRNA. Translation: AAI05305.1.
RefSeqiNP_001014248.2. NM_001014226.2.
UniGeneiRn.13255.

3D structure databases

ProteinModelPortaliQ3MHC1.
SMRiQ3MHC1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009936.

Proteomic databases

PaxDbiQ3MHC1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009936; ENSRNOP00000009936; ENSRNOG00000007280.
ENSRNOT00000075238; ENSRNOP00000064770; ENSRNOG00000007280.
GeneIDi363545.
KEGGirno:363545.
UCSCiRGD:1308915. rat.

Organism-specific databases

CTDi79903.
RGDi1308915. Naa60.

Phylogenomic databases

eggNOGiENOG410IQK0. Eukaryota.
ENOG410Y94A. LUCA.
GeneTreeiENSGT00390000008314.
HOGENOMiHOG000280717.
HOVERGENiHBG061680.
InParanoidiQ3MHC1.
KOiK21121.
OMAiSIPQRLY.
OrthoDBiEOG091G0L3L.
PhylomeDBiQ3MHC1.
TreeFamiTF323980.

Miscellaneous databases

PROiPR:Q3MHC1.

Gene expression databases

BgeeiENSRNOG00000007280.
GenevisibleiQ3MHC1. RN.

Family and domain databases

InterProiView protein in InterPro
IPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
PfamiView protein in Pfam
PF00583. Acetyltransf_1. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiView protein in PROSITE
PS51186. GNAT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNAA60_RAT
AccessioniPrimary (citable) accession number: Q3MHC1
Secondary accession number(s): Q7TMZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 25, 2005
Last modified: June 7, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.