Reviewed, UniProtKB/Swiss-Prot Q3MH53 (PROA_ANAVT)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Ava_0057

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000229992

Sequences

	Sequence		Length	Mass (Da)
	Q3MH53-1 [UniParc]. Last modified April 4, 2006. Version 2. Checksum: 3393756F7C3684DA	FASTA	434	46,945
	10 20 30 40 50 60 MTTLQVASSL NDIAQQTRQA ASLLAMLSTE AKNQAIAAVA QALESAKEEI LQANIADCEA 70 80 90 100 110 120 ATAEGIAKPL YKRLQLDEHK LRDAIAGVRD VGKLADPIGQ VQIQRELDTG LVLKRITCPL 130 140 150 160 170 180 GVLGIIFEAR PEAAIQIISL AIKSGNGVIL KCGKEAVRSC EAIVKAVKQG LSTTDVNPDV 190 200 210 220 230 240 VQLLTTREET LELLRLDKYV DLIIPRGSNS FVRFVQENTR IPVLGHADGI CHVYIDKSAD 250 260 270 280 290 300 IEKAIAVSVD AKVQYPAACN AIETLLVHHS IAAEFLPKVA QALAERQVEL KGDERTLQIL 310 320 330 340 350 360 PEIAAATAID WETEYSDFIL SIKIVDSLTE AIAHINQYGS RHTDAIITED VAAVETFFGL 370 380 390 400 410 420 VNSAGVFHNC STRFADGFRY GFGAEVGIST QQMPPRGPVG LEGLVTYKYQ MTGTGHIVAT 430 YTGENAKPFT HQDF

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA19683.1. Different initiation.
RefSeq	YP_320578.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3683469.
GenomeReviews	Gene locus Ava_0057 in contig CP000117_GR.
KEGG	ava:Ava_0057.
NMPDR	fig|240292.3.peg.363.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MH53.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_0057-MON.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ANAVT

Accession

Primary (citable) accession number: Q3MH53

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	April 4, 2006
Last modified:	June 16, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)