Reviewed, UniProtKB/Swiss-Prot Q3MGX7 (ILVC_ANAVT)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Ava_0133

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000252749
Sites
	Active site	108	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3MGX7-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 3AF38044D6CE7F1C	FASTA	331	35,997
	10 20 30 40 50 60 MARMYYDEDA NLDLLAGKTI AIIGYGSQGH AHALNLKDSG LNVIVGLYPG SKSAEKAQAA 70 80 90 100 110 120 GLTVKNVADA ANVADFIMIL LPDEVQKTVY KNEIEPNLQE GNTLAFAHGF NIHFGQVVPP 130 140 150 160 170 180 ANVDVVMVAP KGPGHLVRRT YEQGQGVPAL FAVYQDASGK ARDRALSYAK GIGGTRGGVL 190 200 210 220 230 240 ETTFREETET DLFGEQAVLC GGLSALIKAG FETLVEAGYQ PELAYFECLH EVKLIVDLVV 250 260 270 280 290 300 EGGLAKMRDS ISNTAEYGDY TRGPRIVNEQ TKAEMRKVLS EIQSGQFARE FVLENQSGKP 310 320 330 GFTAMRRQEA EHPIEEVGKD LRAMFSWLKK V

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA19759.1.
RefSeq	YP_320654.1.
3D structure databases
HSSP	HSSP built from PDB template 1YRL based on UniProtKB P05793.
SMR	Q3MGX7. Positions 3-328.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3MGX7.
Genome annotation databases
GeneID	3683350.
GenomeReviews	Gene locus Ava_0133 in contig CP000117_GR.
KEGG	ava:Ava_0133.
NMPDR	fig|240292.3.peg.526.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0059.
HOGENOM	HBG286525.
OMA	DSISNTA.
PhylomeDB	Q3MGX7.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_0133-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_ANAVT

Accession

Primary (citable) accession number: Q3MGX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	October 25, 2005
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)