Q3MGL3 (SYD_ANAVT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Ava_0247

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_0000235505

Sequences

	Sequence		Length	Mass (Da)
	Q3MGL3 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 977CD7F03977C7E1	FASTA	614	70,073
	10 20 30 40 50 60 MGRLWHSLAN LARKNKLTTM RTHYCGELRQ KDIGETVTLY GWVDRRRDHG GVIFLDLRDR 70 80 90 100 110 120 SGIVQVVSDP QRTPDSYELA NSLRNEYVVE ITGRVTQRPE ESLNSRIPTG EVEIYADKIE 130 140 150 160 170 180 LLNGVRKQLP FQVSTADTET VREDLRLKYR YLDLRRDRMA RNIQLRHQVV KAMRRYLEDV 190 200 210 220 230 240 EGFIEVETPI LTRSTPEGAR DYVLPSRVNP GEWFALPQSP QLFKQILMVS GMDRYYQIAR 250 260 270 280 290 300 CFRDEDLRAD RQPEFTQLDM EMSFMSEEEI IQLNEKLVSY IFKTVKGVEL PLPFPRLTYA 310 320 330 340 350 360 EAMERYGCDK PDTRYDLQLV NVSDVMKDSG FKVFRDAVAN GGIVKILPIP NGNEQISNVR 370 380 390 400 410 420 IKPGGDLFRE ASEAGAKGLA YIRVREDGEI DTIGAIKDNL SEEQKQEILQ RTGAKPGHLL 430 440 450 460 470 480 LFGAGDAATV NKTLDRLRQA IAKEFGLIDP DKINLLWVVD FPMFEWNADE KRLEALHHPF 490 500 510 520 530 540 TAPHPDDLHD LKTARAQAYD LVFNGFEVGG GSRRIYQREV QEQVFETIGL SPEEAQNKFG 550 560 570 580 590 600 FLLEAFEYGT PPHGGIAYGL DRLVMLFAGE ESIRDVIAFP KTQQARCLLT DAPSGVDVKQ 610 LKELHVASTY KPKS

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29413 / PCC 7937.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA19873.1.
RefSeq	YP_320768.1. NC_007413.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3MGL3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3683011.
GenomeReviews	Gene locus Ava_0247 in contig CP000117_GR.
KEGG	ava:Ava_0247.
NMPDR	fig|240292.3.peg.730.
PATRIC	35420785. VBIAnaVar43351_0966.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0173.
HOGENOM	HBG396032.
OMA	AFPKTQQ.
PhylomeDB	Q3MGL3.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_0247-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ANAVT

Accession

Primary (citable) accession number: Q3MGL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	October 25, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program