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Protein

Hydroxyacylglutathione hydrolase

Gene

gloB

Organism
Anabaena variabilis (strain ATCC 29413 / PCC 7937)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.UniRule annotation

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Zinc 1UniRule annotation
Metal bindingi56 – 561Zinc 1UniRule annotation
Metal bindingi58 – 581Zinc 2UniRule annotation
Metal bindingi59 – 591Zinc 2UniRule annotation
Metal bindingi113 – 1131Zinc 1UniRule annotation
Metal bindingi137 – 1371Zinc 1UniRule annotation
Metal bindingi137 – 1371Zinc 2UniRule annotation
Metal bindingi175 – 1751Zinc 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciAVAR240292:GCY3-329-MONOMER.
UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolaseUniRule annotation (EC:3.1.2.6UniRule annotation)
Alternative name(s):
Glyoxalase IIUniRule annotation
Short name:
Glx IIUniRule annotation
Gene namesi
Name:gloBUniRule annotation
Ordered Locus Names:Ava_0328
OrganismiAnabaena variabilis (strain ATCC 29413 / PCC 7937)
Taxonomic identifieri240292 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena
Proteomesi
  • UP000002533 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Hydroxyacylglutathione hydrolasePRO_1000068207Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi240292.Ava_0328.

Structurei

3D structure databases

ProteinModelPortaliQ3MGD2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108RW0. Bacteria.
COG0491. LUCA.
HOGENOMiHOG000058041.
KOiK01069.
OMAiDNYIFLL.
OrthoDBiPOG091H03YM.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3MGD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVIRLAALS DNYIFLLHDS QKNIAAVVDP AEAEPVLKQL AQLNAELVAI
60 70 80 90 100
FNTHHHNDHV GGNQQLIQNF PQLKVYGGAE DKGRIPGQQV FLQPGDRVQF
110 120 130 140 150
TDRVAEVIFV PGHTRAHIAY YFPPQTADTP GELFCGDTLF AGGCGRLFEG
160 170 180 190 200
TPAQMVESLT KLRSLPENTR VWCAHEYTLK NLQFALSVDS ENTELQKRFD
210 220 230 240 250
EVKTKRSQGI ATVPSLLGVE KLTNPFLRWE QPSLQSAVNS NDPVQTFARI

RGLKDKF
Length:257
Mass (Da):28,694
Last modified:October 25, 2005 - v1
Checksum:i50E7FFAD5137E6E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000117 Genomic DNA. Translation: ABA19954.1.

Genome annotation databases

EnsemblBacteriaiABA19954; ABA19954; Ava_0328.
KEGGiava:Ava_0328.
PATRICi35420965. VBIAnaVar43351_1056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000117 Genomic DNA. Translation: ABA19954.1.

3D structure databases

ProteinModelPortaliQ3MGD2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi240292.Ava_0328.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA19954; ABA19954; Ava_0328.
KEGGiava:Ava_0328.
PATRICi35420965. VBIAnaVar43351_1056.

Phylogenomic databases

eggNOGiENOG4108RW0. Bacteria.
COG0491. LUCA.
HOGENOMiHOG000058041.
KOiK01069.
OMAiDNYIFLL.
OrthoDBiPOG091H03YM.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.
BioCyciAVAR240292:GCY3-329-MONOMER.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLO2_ANAVT
AccessioniPrimary (citable) accession number: Q3MGD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 25, 2005
Last modified: September 7, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.