Reviewed, UniProtKB/Swiss-Prot Q3MGA0 (DDL_ANAVT)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	Ava_0360

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	D-alanine--D-alanine ligase		PRO_1000030423
Regions
	Domain	148 – 357	210	ATP-grasp
	Nucleotide binding	184 – 239	56	ATP By similarity
Sites
	Metal binding	310	1	Magnesium or manganese 1 By similarity
	Metal binding	324	1	Magnesium or manganese 1 By similarity
	Metal binding	324	1	Magnesium or manganese 2 By similarity
	Metal binding	326	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MGA0-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: C7E4A822197A0DC5	FASTA	367	39,782
	10 20 30 40 50 60 MSKLRVGLLF GGRSGEHEVS ISSARAIASA LSAGENASKY EILPFYIHKD GRWLAGEAPQ 70 80 90 100 110 120 QVLKSGAPLL ESSNSSPADN NLVNSQQQTL ERWQSPSQVA EVDVWFPILH GPNGEDGTIQ 130 140 150 160 170 180 GLLTLMQTPF VGSGVLGSAL GMDKIAMKMA FEQAGLPQVK YKAVTRAQIW SNPCVFPKLC 190 200 210 220 230 240 DEIEASLGYP CFVKPANLGS SVGISKVRSR QELEDALDNA ANYDRRIIVE AGVVAREVEC 250 260 270 280 290 300 AVLGNDQPQA STVGEITFNS DFYDYETKYT AGKADLLIPA VIPDEISRQI QDMALQAFAA 310 320 330 340 350 360 VDAAGLARVD FFYVEATGEV LINEINTLPG FTATSMYPQL WAYSGIPFPE LVDKLVQLAI ERHNPSH

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA19986.1.
RefSeq	YP_320881.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3682734.
GenomeReviews	Gene locus Ava_0360 in contig CP000117_GR.
KEGG	ava:Ava_0360.
NMPDR	fig|240292.3.peg.961.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MGA0.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_0360-MON.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_ANAVT

Accession

Primary (citable) accession number: Q3MGA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2005
Last modified:	November 25, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)