Reviewed, UniProtKB/Swiss-Prot Q3MFQ5 (PDXH_ANAVT)

Names and origin

Protein names

Recommended name:
    Pyridoxine/pyridoxamine 5'-phosphate oxidase
    EC=1.4.3.5
Alternative name(s):
    PNP/PMP oxidase
      Short name=PNPOx
    Pyridoxal 5'-phosphate synthase

Gene names

Name:	pdxH
Ordered Locus Names:	Ava_0557

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP) By similarity. HAMAP MF_01629
Catalytic activity	Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2. HAMAP MF_01629 Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2. HAMAP MF_01629
Cofactor	Binds 1 FMN per subunit By similarity. HAMAP MF_01629
Pathway	Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1. HAMAP MF_01629 Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.
Subunit structure	Homodimer By similarity. HAMAP MF_01629
Sequence similarities	Belongs to the pyridoxamine 5'-phosphate oxidase family.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: HAMAP pyridoxamine-phosphate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Pyridoxine/pyridoxamine 5'-phosphate oxidase HAMAP MF_01629		PRO_0000255850
Regions
	Nucleotide binding	77 – 78	2	FMN By similarity
	Nucleotide binding	141 – 142	2	FMN By similarity
	Region	9 – 12	4	Substrate binding By similarity
	Region	192 – 194	3	Substrate binding By similarity
Sites
	Binding site	62	1	FMN By similarity
	Binding site	65	1	FMN; via amide nitrogen By similarity
	Binding site	67	1	Substrate By similarity
	Binding site	84	1	FMN By similarity
	Binding site	124	1	Substrate By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MFQ5-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 370A98CBBD49CAAE	FASTA	214	25,160
	10 20 30 40 50 60 MDRTIADLRK DYTLEALSEV EVDTNPFRQF KRWFEQALAA QLPEPNAMTI ATSTPDGQPS 70 80 90 100 110 120 ARMVLLKDFD ERGFVFFTNY NSRKGQELAE NPQAALVFWW AELERQVRIS GRVEKVSESE 130 140 150 160 170 180 SDYYFYSRPA NSRLGAWVSN QSEIIASREV LEQRMQEFQH KYENQEIPRP SHWGGLRVIP 190 200 210 SQIEFWQGRS SRLHDRLLYT LLNDDSWEIH RLSP

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA20181.1.
RefSeq	YP_321076.1.
3D structure databases
HSSP	HSSP built from PDB template 2A2J based on UniProtKB P65682.
SMR	Q3MFQ5. Positions 16-214.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3MFQ5.
Genome annotation databases
GeneID	3682329.
GenomeReviews	Gene locus Ava_0557 in contig CP000117_GR.
KEGG	ava:Ava_0557.
NMPDR	fig|240292.3.peg.2485.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0259.
HOGENOM	HBG327559.
OMA	FTFFTNY.
PhylomeDB	Q3MFQ5.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_0557-MONOMER.
Family and domain databases
HAMAP	MF_01629. PdxH. [Tree]
InterPro	IPR000659. Pyridoxamine_oxidase. IPR019740. Pyridoxamine_oxidase_CS. IPR019576. Pyridoxamine_oxidase_dimer_C. IPR011576. PyridoxamineP_oxidase_FMN-bd. IPR009002. Split_barrel_FMN-bd_related. IPR012349. Split_barrel_FMN_bd. [Graphical view]
Gene3D	G3DSA:2.30.110.10. PNPOx_FMN_bd. 1 hit.
PANTHER	PTHR10851. Pyridox_oxidase. 1 hit.
Pfam	PF10590. PNPOx_C. 1 hit. PF01243. Pyridox_oxidase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00558. pdxH. 1 hit.
PROSITE	PS01064. PYRIDOX_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDXH_ANAVT

Accession

Primary (citable) accession number: Q3MFQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 25, 2005
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)