Reviewed, UniProtKB/Swiss-Prot Q3MF40 (PGK_ANAVT)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate kinase
    EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	Ava_0772

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP MF_00145
Subunit structure	Monomer By similarity. HAMAP MF_00145
Subcellular location	Cytoplasm Potential HAMAP MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Phosphoglycerate kinase HAMAP MF_00145		PRO_1000057959
Regions
	Nucleotide binding	356 – 359	4	ATP By similarity
	Region	24 – 26	3	Substrate binding By similarity
	Region	63 – 66	4	Substrate binding By similarity
Sites
	Binding site	40	1	Substrate By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	205	1	ATP By similarity
	Binding site	296	1	ATP; via carbonyl oxygen By similarity
	Binding site	327	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MF40-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 3B7DF324A5A21178	FASTA	400	42,441
	10 20 30 40 50 60 MSKKTVASLS AADISGKRAL VRVDFNVPLD DQGNITDDTR IRAALPTIQD LTQKGAKVIL 70 80 90 100 110 120 ASHFGRPKGV DEKLRLTPVA KRLSELLGQE VVKTDDSIGD EVAAKVAALQ NGQVLLLENV 130 140 150 160 170 180 RFYKEEEKND PEFAKKLAAN ADFYVNDAFG TAHRAHASTE GVTKFLSPSV AGYLVEKELQ 190 200 210 220 230 240 YLQSAIENPQ RPLAAIIGGS KVSSKIGVIE TLLEKCDKLI IGGGMIFTFY KARGLNVGKS 250 260 270 280 290 300 LVEEDKLELA KSLEAKAKER GVSLLLPTDV VLADNFAPDA NSQTVSIDNI PDGWMGLDIG 310 320 330 340 350 360 PDSVKVFQEA LADTKTVIWN GPMGVFEFDK FAAGTEAIAH TLAEIGKTGT TTIIGGGDSV 370 380 390 400 AAVEKVGLAD QMSHISTGGG ASLELLEGKV LPGIAALDEA

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA20396.1.
RefSeq	YP_321291.1.
3D structure databases
SMR	Q3MF40. Positions 1-399.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3MF40.
Genome annotation databases
GeneID	3680359.
GenomeReviews	Gene locus Ava_0772 in contig CP000117_GR.
KEGG	ava:Ava_0772.
NMPDR	fig|240292.3.peg.2565.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HBG453500.
OMA	EMTFIST.
PhylomeDB	Q3MF40.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_0772-MONOMER.
Family and domain databases
HAMAP	MF_00145. Phosphoglyc_kinase. [Tree]
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_ANAVT

Accession

Primary (citable) accession number: Q3MF40

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 25, 2005
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)