Reviewed, UniProtKB/Swiss-Prot Q3MF19 (MURD_ANAVT)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	Ava_0793

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000257164
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3MF19-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: BC47CCF630D44A46	FASTA	455	49,411
	10 20 30 40 50 60 MSKAHVVGLG KSGVAAARLL KREGWEVVLS DRNTSDTLLK QQQELAKEQI TVELGYSLDF 70 80 90 100 110 120 AGALPDLIVV SPGVPWDIPA LVKARELGIE TIGEMELAWR HLKSLPWVGI TGTNGKTTTT 130 140 150 160 170 180 ALIAAIFQAA GFDAPACGNI GYAACEVALA ATPPDWIIGE MSSYQIESSL TLAPHISIWT 190 200 210 220 230 240 TFTPDHLARH KTLENYYDIK AKLLRQSHLQ VFNGDDAYLS KIGASHWPDA YWTSVKGKDY 250 260 270 280 290 300 LIGDKGFYIE DGWVVEQLQP NSSPQRIVAA EALRMVGAHN LQNLLMAVAA ARLADIPPNA 310 320 330 340 350 360 IDKAVREFPG VAHRLEHICT WQGIDFINDS KATNYDAAEV GLASVKSPVI LIAGGEAKPG 370 380 390 400 410 420 DDAAWLAKIQ GQTSAVLLIG NAAPAFAQRL QEIGYSNYEI VETMEQAVRR SLDLAKHHQA 430 440 450 PVVLLSPACA SFDQYPNFEA RGDHFRQLCL ELVGS

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA20417.1.
RefSeq	YP_321312.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3680732.
GenomeReviews	Gene locus Ava_0793 in contig CP000117_GR.
KEGG	ava:Ava_0793.
NMPDR	fig|240292.3.peg.2586.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MF19.
OMA	Q3MF19. IRSFAGM.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_0793-MON.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_ANAVT

Accession

Primary (citable) accession number: Q3MF19

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)