Reviewed, UniProtKB/Swiss-Prot Q3MEN7 (SYY_ANAVT)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	Ava_0925

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Tyrosyl-tRNA synthetase HAMAP MF_02007		PRO_0000236690
Regions
	Domain	334 – 398	65	S4 RNA-binding
	Motif	48 – 57	10	"HIGH" region HAMAP MF_02007
	Motif	235 – 239	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	238	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MEN7-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 85E4BC864466B926	FASTA	398	44,447
	10 20 30 40 50 60 MAENFSWLHR GIAEVFPQPT DAESDIESLE KRLATTDRPL RVKYGIDPTG ADIHLGHSIP 70 80 90 100 110 120 MRKLRGFQDA GHTAVLIIGD FTARIGDPTG KSEMRRQLTE EDVKQNAQTY LDQVRPILDF 130 140 150 160 170 180 DTPGRLEVRY NSEWLSRLDL GKITELLATM TVGQMLAKEG FADRYKKENP IFLHEFLYPL 190 200 210 220 230 240 MQGYDSVAIE ADVELGGTDQ KFNIAVGRDL QRHFGQKPQF GVLLPILIGT DGVQKMSKSL 250 260 270 280 290 300 GNYVSLSEHP GQKYQKLQGV PDQMLEQYFE LLTDLPIDKL PANPRDRQML LAWEIVKQYH 310 320 330 340 350 360 GEQAAQEAKE AAQSGGKEGA VPEFSLAGVP QFPVKLAYLL GATGLCKSTG EGKRKIQEGG 370 380 390 VRLDGDRISD ADTIFQQPDE LQGRVLQVGK NKFVRLVL

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA20549.1.
RefSeq	YP_321444.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3681299.
GenomeReviews	Gene locus Ava_0925 in contig CP000117_GR.
KEGG	ava:Ava_0925.
NMPDR	fig|240292.3.peg.131.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MEN7.
OMA	Q3MEN7. YVVQVGK.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_0925-MON.
Family and domain databases
HAMAP	MF_02007. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_ANAVT

Accession

Primary (citable) accession number: Q3MEN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)