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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Anabaena variabilis (strain ATCC 29413 / PCC 7937)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotationSAAS annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotationSAAS annotation
Orotidine 5'-phosphate = UMP + CO2.SAAS annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 and 2 of the subpathway that synthesizes UMP from orotate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE), Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF), Orotate phosphoribosyltransferase (pyrE)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei3745-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei3755-phosphoribose 1-diphosphateUniRule annotation1
Binding sitei3785-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei3805-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylaseSAAS annotation, GlycosyltransferaseUniRule annotationSAAS annotationImported, Lyase, Transferase
Biological processPyrimidine biosynthesisUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotation

Enzyme and pathway databases

BioCyciAVAR240292:G7WH-8035-MONOMER.
UniPathwayiUPA00070; UER00119.
UPA00070; UER00120.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:Ava_0957Imported
OrganismiAnabaena variabilis (strain ATCC 29413 / PCC 7937)Imported
Taxonomic identifieri240292 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena
Proteomesi
  • UP000002533 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi240292.Ava_0957.

Structurei

3D structure databases

ProteinModelPortaliQ3MEK5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 224OMPdecaseInterPro annotationAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni400 – 4085-phosphoribose 1-diphosphate bindingUniRule annotation9

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108DJF. Bacteria.
COG0284. LUCA.
COG0461. LUCA.
HOGENOMiHOG000256439.
KOiK13421.
OMAiMIFPRKE.
OrthoDBiPOG091H034Q.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR011995. OMPdecase_type-2.
IPR001754. OMPdeCOase_dom.
IPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR011060. RibuloseP-bd_barrel.
PfamiView protein in Pfam
PF00215. OMPdecase. 1 hit.
PF00156. Pribosyltran. 1 hit.
SMARTiView protein in SMART
SM00934. OMPdecase. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
TIGR02127. pyrF_sub2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3MEK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFSDKLHQK ISQNQSLLFV GLDPNPEMMP GRYSSEDIID GLWNWLQFII
60 70 80 90 100
AETADFVCAY KPTLGFYEAL GVPGLELLQK TLGAIPSHIP IILDAKHSDL
110 120 130 140 150
NTSTIFAKTV FTEWGVDAIT LSPYTGQDHV APFLVYPDKA VFILCSTSNP
160 170 180 190 200
GAEALQQYPT RESPLYLQVV QESKNWGTPE QLGLEVGTTN AEVLARIRQV
210 220 230 240 250
APERIIMARS IWAEGGNLNR ILEVGLDTDG NGLLIPVPQD MLASANLSKE
260 270 280 290 300
IQSLRAEINQ VRDITVRDVS SCSVWLPDVS TVNQHPLHDL ILQLYDIDCI
310 320 330 340 350
MFGNFVQASG AVFPYYIDLR KIISNPQVFN QVLSGYENIV KNLTFDRLAG
360 370 380 390 400
IPYGSLPTAT GLALRLNCPM IFPRKEVKAH GTRRLIEGNF HPGELVVVVD
410 420 430 440 450
DILISGKSVM EGADKLKSAG LNVHDIVVFI DHEQGVKDKL QANGYRGHAV
460 470
LTISEITSVL HQAGRINDEQ FLALTAE
Length:477
Mass (Da):52,654
Last modified:October 25, 2005 - v1
Checksum:iABE18E915905D203
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000117 Genomic DNA. Translation: ABA20581.1.

Genome annotation databases

EnsemblBacteriaiABA20581; ABA20581; Ava_0957.
KEGGiava:Ava_0957.

Similar proteinsi

Entry informationi

Entry nameiQ3MEK5_ANAVT
AccessioniPrimary (citable) accession number: Q3MEK5
Entry historyiIntegrated into UniProtKB/TrEMBL: October 25, 2005
Last sequence update: October 25, 2005
Last modified: September 27, 2017
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported