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Q3ME15

- MURE_ANAVT

UniProt

Q3ME15 - MURE_ANAVT

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Protein
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
Gene
murE, Ava_1147
Organism
Anabaena variabilis (strain ATCC 29413 / PCC 7937)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321UDP-MurNAc-L-Ala-D-Glu By similarity
Binding sitei185 – 1851UDP-MurNAc-L-Ala-D-Glu By similarity
Binding sitei191 – 1911UDP-MurNAc-L-Ala-D-Glu By similarity
Binding sitei193 – 1931UDP-MurNAc-L-Ala-D-Glu By similarity
Binding sitei389 – 3891Meso-diaminopimelate By similarity
Binding sitei464 – 4641Meso-diaminopimelate; via carbonyl oxygen By similarity
Binding sitei468 – 4681Meso-diaminopimelate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi116 – 1227ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  4. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAVAR240292:GCY3-1159-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (EC:6.3.2.13)
Alternative name(s):
Meso-A2pm-adding enzyme
Meso-diaminopimelate-adding enzyme
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UDP-MurNAc-tripeptide synthetase
UDP-N-acetylmuramyl-tripeptide synthetase
Gene namesi
Name:murE
Ordered Locus Names:Ava_1147
OrganismiAnabaena variabilis (strain ATCC 29413 / PCC 7937)
Taxonomic identifieri240292 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena
ProteomesiUP000002533: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation
PRO_1000012335Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei225 – 2251N6-carboxylysine By similarity

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.UniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi240292.Ava_1147.

Structurei

3D structure databases

ProteinModelPortaliQ3ME15.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni158 – 1592UDP-MurNAc-L-Ala-D-Glu binding By similarity
Regioni413 – 4164Meso-diaminopimelate binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi413 – 4164Meso-diaminopimelate recognition motifUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0769.
HOGENOMiHOG000268118.
KOiK01928.
OMAiVDYAHTG.
OrthoDBiEOG6PKFCR.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00208. MurE.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01085. murE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3ME15-1 [UniParc]FASTAAdd to Basket

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MKLRELLATV DSVENLPPVL ADTEVKGIKT NSHACGAGDL FIGMPGTRVD    50
GGEFWPSAIA SGAIAAIVSP QAVEKNPPSD EAVVISSNNM TKACAAIAAA 100
FYGYPGQKLK LVGVTGTNGK TTTTHLIEFF LTKAQLSTAL MGTLYTRWPG 150
FEQTATHTTP FAVELQQQLA QAVNAGCEFG VMEVSSHALA QGRVLGCPFE 200
VGVFSNLTQD HLDYHSDMED YFAAKALLFS PDYLKGRAII NADDTYGQRL 250
IKALSPEKVW SYSVNDSSAD LWMSDLSYEP NGVTGTIHTP EGDVSFRSPL 300
VGQYNLENLL ASVGAVLHLG LDLQLIANAI PEFPGVPGRM ERVQINPDQD 350
ISVIVDYAHT PDSLENLLKA ARPFIPGRMI CVFGCGGDRD RTKRPKMGKI 400
AAELADLAFV TSDNPRTEDP EKILDDILAG IPDTVQPTVI GDRAIAIRTA 450
ILQAQPGDGV LLAGKGHEDY QILGTEKIHF DDREHARAAL TEREKL 496
Length:496
Mass (Da):53,416
Last modified:October 25, 2005 - v1
Checksum:iDCDF7907B96B79BA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000117 Genomic DNA. Translation: ABA20771.1.
RefSeqiWP_011317994.1. NC_007413.1.
YP_321666.1. NC_007413.1.

Genome annotation databases

EnsemblBacteriaiABA20771; ABA20771; Ava_1147.
GeneIDi3683401.
KEGGiava:Ava_1147.
PATRICi35422808. VBIAnaVar43351_1967.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000117 Genomic DNA. Translation: ABA20771.1 .
RefSeqi WP_011317994.1. NC_007413.1.
YP_321666.1. NC_007413.1.

3D structure databases

ProteinModelPortali Q3ME15.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 240292.Ava_1147.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA20771 ; ABA20771 ; Ava_1147 .
GeneIDi 3683401.
KEGGi ava:Ava_1147.
PATRICi 35422808. VBIAnaVar43351_1967.

Phylogenomic databases

eggNOGi COG0769.
HOGENOMi HOG000268118.
KOi K01928.
OMAi VDYAHTG.
OrthoDBi EOG6PKFCR.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci AVAR240292:GCY3-1159-MONOMER.

Family and domain databases

Gene3Di 3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPi MF_00208. MurE.
InterProi IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view ]
Pfami PF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view ]
SUPFAMi SSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsi TIGR01085. murE. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Anabaena variabilis ATCC 29413."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29413 / PCC 7937.

Entry informationi

Entry nameiMURE_ANAVT
AccessioniPrimary (citable) accession number: Q3ME15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 25, 2005
Last modified: September 3, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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