Reviewed, UniProtKB/Swiss-Prot Q3MDU0 (PYRD_ANAVT)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	Ava_1222

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000024151
Sites
	Active site	193	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MDU0-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 0C58CDA5D2A728E8	FASTA	376	41,318
	10 20 30 40 50 60 MDIYKFAVRP LLFDLVKADP EWLHQQTMRS LSWLSHTSDR TSTKWVQNIL QKSLCIEDSR 70 80 90 100 110 120 LEQNLFGLRF PNPVGLAAGF DKDGVAARIW SSLGFGFAEL GTVTFVAQPG NPPPRLFRLP 130 140 150 160 170 180 LDQAALNRMG FNNHGAAVMA TRLADEKGLF SIPIGINLGK SKVTPLEAAA EDYLNSFRLL 190 200 210 220 230 240 KELGDYFVVN VSSPNTPGLR SLQDASMLSS ILDVLQKENQ SHKPIFVKIA PDLEWEAIAD 250 260 270 280 290 300 IIGLAKTYQL AGIIATNTTI RRDGLKTQVI EQTGKAPQEE AGGISGAPVR DRSTEIIRFI 310 320 330 340 350 360 WQQTQGEIPI IGVGGIFTPE DAWAKITAGA SLIQVYTGWI YQGPMMVSQI LTGLLAKLEE 370 HELNSISEAI GLEFKS

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA20846.1.
RefSeq	YP_321741.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3MDU0.
Genome annotation databases
GeneID	3683262.
GenomeReviews	Gene locus Ava_1222 in contig CP000117_GR.
KEGG	ava:Ava_1222.
NMPDR	fig|240292.3.peg.1906.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MDU0.
OMA	AALNRMG.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_1222-MON.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_ANAVT

Accession

Primary (citable) accession number: Q3MDU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2005
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)