Reviewed, UniProtKB/Swiss-Prot Q3MDN9 (MDH_ANAVT)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Ava_1273

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Malate dehydrogenase HAMAP MF_00487		PRO_0000241938
Regions
	Nucleotide binding	21 – 26	6	NAD By similarity
	Nucleotide binding	130 – 132	3	NAD By similarity
Sites
	Active site	187	1	Proton acceptor By similarity
	Binding site	45	1	NAD By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	107	1	NAD By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	163	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MDN9-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: E6C8A990378DCF39	FASTA	324	34,804
	10 20 30 40 50 60 MSSSPDSPPI LHRLPRVAII GAGRVGSTLA QRIAEKNLAD VVLLDIVEGM PQGLALDLLE 70 80 90 100 110 120 ARGIELHNRQ IIGTNNYADT SGSQIVVITA GFPRKPGMSR DDLLRTNAKI VVEAAKQAIA 130 140 150 160 170 180 YSPCAIFIVV TNPLDVMTYL AWEATGLPRN RIMGMAGVLD SARFETFIAL ELGVLPADVK 190 200 210 220 230 240 AMVLGSHGDL MVPLSRHATV NGIPITELLD AATIERLVER TRNGGAEIVE LMQTGGAFFA 250 260 270 280 290 300 PASATSLMVE SILLNQSRLL PVSVYLQGEY GLKDVVIGVP CRLGLNGIES VIELNLSDSE 310 320 REALQTSAQS VQKNIERWHS TQHS

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA20897.1.
RefSeq	YP_321792.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3683113.
GenomeReviews	Gene locus Ava_1273 in contig CP000117_GR.
KEGG	ava:Ava_1273.
NMPDR	fig|240292.3.peg.1957.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MDN9.
OMA	Q3MDN9. CINIAPS.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_1273-MON.
Family and domain databases
HAMAP	MF_00487. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_NAD-dep_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_ANAVT

Accession

Primary (citable) accession number: Q3MDN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)