Reviewed, UniProtKB/Swiss-Prot Q3MDL2 (DXR_ANAVT)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose 5-phosphate reductoisomerase
      Short name=DXP reductoisomerase
    EC=1.1.1.267
Alternative name(s):
    1-deoxyxylulose-5-phosphate reductoisomerase
    2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	Ava_1300

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00183
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity. HAMAP MF_00183
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_1000020213
Regions
	Nucleotide binding	7 – 36	30	NADP By similarity
Sites
	Metal binding	150	1	Divalent metal cation By similarity
	Metal binding	152	1	Divalent metal cation By similarity
	Metal binding	221	1	Divalent metal cation By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity
	Binding site	176	1	Substrate By similarity
	Binding site	199	1	Substrate By similarity
	Binding site	221	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MDL2-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 4AA74AB2321D3130	FASTA	398	43,055
	10 20 30 40 50 60 MKSITLVGST GSIGTQTLDI VSQYPDQFRI VGLAAGSNVE MLAEQIRQFR PQIAAISAAE 70 80 90 100 110 120 KLPALQAAIK DLDPQPILLG GEAGVIEVAR YGDAETVVTG IVGCAGLLPT IAAIEAGKDI 130 140 150 160 170 180 ALANKETLIA GGPVVLPLVE KHGVKLLPAD SEHSAIFQCL QGVPKGGLRK ILLTASGGAF 190 200 210 220 230 240 RDWDVERLAE VTVADALKHP NWSMGRKITV DSATLMNKGL EVIEAHFLFG LDYQDIEIVI 250 260 270 280 290 300 HPQSIIHSLI ELQDTSVLAQ LGWPDMRLPL LYALSWPERI YTDWEPLNLV KAGNLTFREP 310 320 330 340 350 360 DHQKYPCMQL AYAAGRAGGS MPAVLNAANE QVVALFLDEK IKFLDIPRCI ELVCDRHQND 370 380 390 NCANPSLDDI LAADQWARQE VLTATKNLAS QPQIISVN

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA20924.1.
RefSeq	YP_321819.1.
3D structure databases
SMR	Q3MDL2. Positions 2-391.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3MDL2.
Genome annotation databases
GeneID	3683043.
GenomeReviews	Gene locus Ava_1300 in contig CP000117_GR.
KEGG	ava:Ava_1300.
NMPDR	fig|240292.3.peg.2145.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0743.
HOGENOM	HBG430762.
OMA	IHSMVEY.
PhylomeDB	Q3MDL2.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_1300-MONOMER.
Family and domain databases
HAMAP	MF_00183. DXP_reductoisom. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_ANAVT

Accession

Primary (citable) accession number: Q3MDL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2005
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)