Reviewed, UniProtKB/Swiss-Prot Q3MCG0 (RISB_ANAVT)

Names and origin

Protein names

Recommended name:
    6,7-dimethyl-8-ribityllumazine synthase
      Short name=DMRL synthase
      Short name=Lumazine synthase
    EC=2.5.1.9
Alternative name(s):
    Riboflavin synthase beta chain

Gene names

Name:	ribH
Ordered Locus Names:	Ava_1704

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_1000040362

Sequences

	Sequence		Length	Mass (Da)
	Q3MCG0-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 1DBA69F59CE54208	FASTA	190	20,681
	10 20 30 40 50 60 MAVFEGTFTQ TEPLRLAIVI GRFNDLVTTK LLAGCQDCLK RHGVDPDPHG NQVDYVWVPG 70 80 90 100 110 120 SFEVPLVARQ LALTHRYDAI ICLGAVIRGQ TPHFDYVSAE VSKGIAAASF QTGVPVIFGI 130 140 150 160 170 180 LTVDTMQQAL ERAGIKANHG WDYAMNALEM ASLMRQLRSN VTDSYTQTQS LPAAFPNASI 190 GKLTAESEEV

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA21326.1.
RefSeq	YP_322221.1.
3D structure databases
HSSP	HSSP built from PDB template 2A59 based on UniProtKB Q9UUB1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3MCG0.
Genome annotation databases
GeneID	3682184.
GenomeReviews	Gene locus Ava_1704 in contig CP000117_GR.
KEGG	ava:Ava_1704.
NMPDR	fig|240292.3.peg.2784.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0054.
HOGENOM	HBG311126.
OMA	LTTDTMQ.
PhylomeDB	Q3MCG0.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_1704-MONOMER.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00114. lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_ANAVT

Accession

Primary (citable) accession number: Q3MCG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2005
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)