Reviewed, UniProtKB/Swiss-Prot Q3MBG1 (PURA_ANAVT)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	Ava_2053

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Adenylosuccinate synthetase HAMAP MF_00011		PRO_1000000775
Regions
	Nucleotide binding	12 – 18	7	GTP Potential
Sites
	Active site	139	1	By similarity
	Active site	146	1	By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MBG1-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: C45B513FAEB7AC80	FASTA	447	49,140
	10 20 30 40 50 60 MANVIVIGAQ WGDEGKGKIT DLLSRSADVV VRYQGGVNAG HTIVVKGQTF KLHLIPSGIL 70 80 90 100 110 120 YPDTECMIGC GTVIDPQVLI KELDQLESLN ISTKNLLISE TAHVTMPYHR LIDKASEERR 130 140 150 160 170 180 GSHKIGTTGR GIGPTYADKS ERTGIRVLDL MDPAALRDQL AWTINNKNLI LEKLYNLPPL 190 200 210 220 230 240 DTEEVVKEYL GYAERLRPHV VDTSLKIYDA IQRRRNILFE GAQGTLLDLD HGTYPYVTSS 250 260 270 280 290 300 NPVAGGACVG TGLGPTMIDR VIGVSKAYTT RVGEGPFPTE LDGELGELLC DRGAEFGTTT 310 320 330 340 350 360 GRKRRCGWFD AVIGRYAVRI NGMDCMAITK LDVLDELEEI QVCIAYEIDG DRCDHFPTSA 370 380 390 400 410 420 RQFARCRPIY KTLPGWQVPT SECRTLEDLP QQALDYLKFL AELMEVPIAI VSLGASRDQT 430 440 IIVEDPIHGP KRALLHPDGT PASLLSA

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA21675.1.
RefSeq	YP_322570.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3MBG1.
Genome annotation databases
GeneID	3680702.
GenomeReviews	Gene locus Ava_2053 in contig CP000117_GR.
KEGG	ava:Ava_2053.
NMPDR	fig|240292.3.peg.3133.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MBG1.
OMA	IPVCVAY.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_2053-MON.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_ANAVT

Accession

Primary (citable) accession number: Q3MBG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)