ID   ASSY_ANAVT              Reviewed;         400 AA.
AC   Q3MBE6;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   16-JUN-2009, entry version 25.
DE   RecName: Full=Argininosuccinate synthase;
DE            EC=6.3.4.5;
DE   AltName: Full=Citrulline--aspartate ligase;
GN   Name=argG; OrderedLocusNames=Ava_2068;
OS   Anabaena variabilis (strain ATCC 29413 / PCC 7937).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Anabaena.
OX   NCBI_TaxID=240292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J.,
RA   Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.;
RT   "Complete sequence of Anabaena variabilis ATCC 29413.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: ATP + L-citrulline + L-aspartate = AMP +
CC       diphosphate + N(omega)-(L-arginino)succinate.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-
CC       arginine from L-ornithine and carbamoyl phosphate: step 2/3.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the argininosuccinate synthase family. Type
CC       1 subfamily.
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DR   EMBL; CP000117; ABA21690.1; -; Genomic_DNA.
DR   RefSeq; YP_322585.1; -.
DR   GeneID; 3680541; -.
DR   GenomeReviews; CP000117_GR; Ava_2068.
DR   KEGG; ava:Ava_2068; -.
DR   NMPDR; fig|240292.3.peg.1347; -.
DR   HOGENOM; Q3MBE6; -.
DR   OMA; Q3MBE6; IAPVREW.
DR   BioCyc; AVAR240292:AVA_2068-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004055; F:argininosuccinate synthase activity; IEA:HAMAP.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0006526; P:arginine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00005; -; 1.
DR   InterPro; IPR001518; Arginosuc_synth.
DR   InterPro; IPR018223; Arginosuc_synth_CS.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   PANTHER; PTHR11587; Arginosuc_synth; 1.
DR   Pfam; PF00764; Arginosuc_synth; 1.
DR   TIGRFAMs; TIGR00032; argG; 1.
DR   PROSITE; PS00564; ARGININOSUCCIN_SYN_1; 1.
DR   PROSITE; PS00565; ARGININOSUCCIN_SYN_2; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding;
KW   Complete proteome; Cytoplasm; Ligase; Nucleotide-binding.
FT   CHAIN         1    400       Argininosuccinate synthase.
FT                                /FTId=PRO_0000263906.
FT   NP_BIND      10     18       ATP (By similarity).
FT   BINDING      38     38       ATP; via amide nitrogen and carbonyl
FT                                oxygen (By similarity).
FT   BINDING      89     89       Citrulline (By similarity).
FT   BINDING     119    119       ATP; via amide nitrogen (By similarity).
FT   BINDING     121    121       Aspartate (By similarity).
FT   BINDING     125    125       Aspartate (By similarity).
FT   BINDING     125    125       Citrulline (By similarity).
FT   BINDING     126    126       Aspartate (By similarity).
FT   BINDING     129    129       Citrulline (By similarity).
FT   BINDING     177    177       Citrulline (By similarity).
FT   BINDING     186    186       Citrulline (By similarity).
FT   BINDING     262    262       Citrulline (By similarity).
FT   BINDING     274    274       Citrulline (By similarity).
SQ   SEQUENCE   400 AA;  43959 MW;  720C4C6C608EF417 CRC64;
     MGRAKKVVLA YSGGVDTSVC IPYLKQEWGV EEVITLAADL GQGDELEPIR EKALKSGASE
     SLVADVKESF IKDYAFPAIQ ANALYENRYP LGTALARPLI AKILVEAAEK YGADAIAHGC
     TGKGNDQVRF DVSCTALNPK LKILAPAREW GMSREATIAY GEKFGIPSPV KKSSPYSIDK
     NLLGRSIEAG ALEDPKFEPP EEIYEMTKAI ADTPNEPEYI EIGFTQGLPT TINGTPKDPV
     ALIQELNQLV GSHGVGRIDM IENRLVGIKS REIYESPAML VLIQAHRDLE SLTLTADVSH
     YKRGIEETYS QIVYNGLWYS PLKAALDAFI QKTQERVSGI VRVKLFKGNA TIVGRWSDSS
     LYTPDLATYG AEDQFDHKAA EGFIYVWGLP TRIWAQQDRG
//