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Q3MBD8

- GLYA_ANAVT

UniProt

Q3MBD8 - GLYA_ANAVT

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Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Anabaena variabilis (strain ATCC 29413 / PCC 7937)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361Pyridoxal phosphateUniRule annotation
Binding sitei56 – 561Pyridoxal phosphateUniRule annotation
Binding sitei58 – 581SubstrateUniRule annotation
Binding sitei65 – 651SubstrateUniRule annotation
Binding sitei66 – 661Pyridoxal phosphateUniRule annotation
Binding sitei122 – 1221Substrate; via carbonyl oxygenUniRule annotation
Binding sitei177 – 1771Pyridoxal phosphateUniRule annotation
Binding sitei205 – 2051Pyridoxal phosphateUniRule annotation
Binding sitei230 – 2301Pyridoxal phosphateUniRule annotation
Binding sitei237 – 2371Pyridoxal phosphateUniRule annotation
Binding sitei263 – 2631Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei363 – 3631Pyridoxal phosphateUniRule annotation

GO - Molecular functioni

  1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, One-carbon metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciAVAR240292:GCY3-2099-MONOMER.
UniPathwayiUPA00193.
UPA00288; UER01023.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
Short name:
SHMTUniRule annotation
Short name:
Serine methylaseUniRule annotation
Gene namesi
Name:glyAUniRule annotation
Ordered Locus Names:Ava_2076
OrganismiAnabaena variabilis (strain ATCC 29413 / PCC 7937)
Taxonomic identifieri240292 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena
ProteomesiUP000002533: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Serine hydroxymethyltransferasePRO_0000234944Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei231 – 2311N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi240292.Ava_2076.

Structurei

3D structure databases

ProteinModelPortaliQ3MBD8.
SMRiQ3MBD8. Positions 8-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1283Substrate bindingUniRule annotation
Regioni355 – 3573Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the SHMT family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0112.
HOGENOMiHOG000239404.
KOiK00600.
OMAiCAYVNVQ.
OrthoDBiEOG6Z0QB2.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00051. SHMT.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERiPTHR11680. PTHR11680. 1 hit.
PfamiPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000412. SHMT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00096. SHMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3MBD8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTRTNSDFLT NSDPAIAGLI NQELQRQRDH LELIASENFT SAAVLAAQGS
60 70 80 90 100
VLTNKYAEGL PGKRYYGGCE FIDKIEQIAI DRAKQLFGAA HANVQPHSGA
110 120 130 140 150
QANFAVFLTL LAPGDKIMGM DLSHGGHLTH GSPVNVSGKW FQVCHYGVSQ
160 170 180 190 200
QTEQLDYDQI RELALRERPK LLICGYSAYP RIIDFEKFRS IADEVGAYLL
210 220 230 240 250
ADIAHIAGLV ASGLHPDPIP YCHVVTTTTH KTLRGPRGGL ILTGDAELGK
260 270 280 290 300
KLDKSVFPGS QGGPLEHVIA GKAVAFGEAL KPEFQGYSAQ VIENARALAN
310 320 330 340 350
QLQNRGLKLV SDGTDNHLML VDLRSVNMTG KRADQLVSEV NITANKNTVP
360 370 380 390 400
FDPQSPFVTS GLRLGSPAMT TRGMGEAEFT EIGNIIADRL LNPDSDTVAQ
410 420
DCKRRVAALC DRFPLYPHLE IPVPVLA
Length:427
Mass (Da):46,272
Last modified:October 25, 2005 - v1
Checksum:i11EF119715721354
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000117 Genomic DNA. Translation: ABA21698.1.
RefSeqiWP_011318867.1. NC_007413.1.
YP_322593.1. NC_007413.1.

Genome annotation databases

EnsemblBacteriaiABA21698; ABA21698; Ava_2076.
GeneIDi3680549.
KEGGiava:Ava_2076.
PATRICi35424891. VBIAnaVar43351_3000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000117 Genomic DNA. Translation: ABA21698.1 .
RefSeqi WP_011318867.1. NC_007413.1.
YP_322593.1. NC_007413.1.

3D structure databases

ProteinModelPortali Q3MBD8.
SMRi Q3MBD8. Positions 8-411.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 240292.Ava_2076.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA21698 ; ABA21698 ; Ava_2076 .
GeneIDi 3680549.
KEGGi ava:Ava_2076.
PATRICi 35424891. VBIAnaVar43351_3000.

Phylogenomic databases

eggNOGi COG0112.
HOGENOMi HOG000239404.
KOi K00600.
OMAi CAYVNVQ.
OrthoDBi EOG6Z0QB2.

Enzyme and pathway databases

UniPathwayi UPA00193 .
UPA00288 ; UER01023 .
BioCyci AVAR240292:GCY3-2099-MONOMER.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPi MF_00051. SHMT.
InterProi IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view ]
PANTHERi PTHR11680. PTHR11680. 1 hit.
Pfami PF00464. SHMT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000412. SHMT. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00096. SHMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Anabaena variabilis ATCC 29413."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29413 / PCC 7937.

Entry informationi

Entry nameiGLYA_ANAVT
AccessioniPrimary (citable) accession number: Q3MBD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: October 25, 2005
Last modified: November 26, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3