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Q3MBD8

- GLYA_ANAVT

UniProt

Q3MBD8 - GLYA_ANAVT

Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Anabaena variabilis (strain ATCC 29413 / PCC 7937)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (25 Oct 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361Pyridoxal phosphateUniRule annotation
    Binding sitei56 – 561Pyridoxal phosphateUniRule annotation
    Binding sitei58 – 581SubstrateUniRule annotation
    Binding sitei65 – 651SubstrateUniRule annotation
    Binding sitei66 – 661Pyridoxal phosphateUniRule annotation
    Binding sitei122 – 1221Substrate; via carbonyl oxygenUniRule annotation
    Binding sitei177 – 1771Pyridoxal phosphateUniRule annotation
    Binding sitei205 – 2051Pyridoxal phosphateUniRule annotation
    Binding sitei230 – 2301Pyridoxal phosphateUniRule annotation
    Binding sitei237 – 2371Pyridoxal phosphateUniRule annotation
    Binding sitei263 – 2631Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
    Binding sitei363 – 3631Pyridoxal phosphateUniRule annotation

    GO - Molecular functioni

    1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciAVAR240292:GCY3-2099-MONOMER.
    UniPathwayiUPA00193.
    UPA00288; UER01023.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
    Short name:
    SHMTUniRule annotation
    Short name:
    Serine methylaseUniRule annotation
    Gene namesi
    Name:glyAUniRule annotation
    Ordered Locus Names:Ava_2076
    OrganismiAnabaena variabilis (strain ATCC 29413 / PCC 7937)
    Taxonomic identifieri240292 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena
    ProteomesiUP000002533: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427Serine hydroxymethyltransferasePRO_0000234944Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei231 – 2311N6-(pyridoxal phosphate)lysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi240292.Ava_2076.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3MBD8.
    SMRiQ3MBD8. Positions 8-411.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni126 – 1283Substrate bindingUniRule annotation
    Regioni355 – 3573Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the SHMT family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0112.
    HOGENOMiHOG000239404.
    KOiK00600.
    OMAiCAYVNVQ.
    OrthoDBiEOG6Z0QB2.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00051. SHMT.
    InterProiIPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view]
    PANTHERiPTHR11680. PTHR11680. 1 hit.
    PfamiPF00464. SHMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000412. SHMT. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00096. SHMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3MBD8-1 [UniParc]FASTAAdd to Basket

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    MTRTNSDFLT NSDPAIAGLI NQELQRQRDH LELIASENFT SAAVLAAQGS    50
    VLTNKYAEGL PGKRYYGGCE FIDKIEQIAI DRAKQLFGAA HANVQPHSGA 100
    QANFAVFLTL LAPGDKIMGM DLSHGGHLTH GSPVNVSGKW FQVCHYGVSQ 150
    QTEQLDYDQI RELALRERPK LLICGYSAYP RIIDFEKFRS IADEVGAYLL 200
    ADIAHIAGLV ASGLHPDPIP YCHVVTTTTH KTLRGPRGGL ILTGDAELGK 250
    KLDKSVFPGS QGGPLEHVIA GKAVAFGEAL KPEFQGYSAQ VIENARALAN 300
    QLQNRGLKLV SDGTDNHLML VDLRSVNMTG KRADQLVSEV NITANKNTVP 350
    FDPQSPFVTS GLRLGSPAMT TRGMGEAEFT EIGNIIADRL LNPDSDTVAQ 400
    DCKRRVAALC DRFPLYPHLE IPVPVLA 427
    Length:427
    Mass (Da):46,272
    Last modified:October 25, 2005 - v1
    Checksum:i11EF119715721354
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000117 Genomic DNA. Translation: ABA21698.1.
    RefSeqiYP_322593.1. NC_007413.1.

    Genome annotation databases

    EnsemblBacteriaiABA21698; ABA21698; Ava_2076.
    GeneIDi3680549.
    KEGGiava:Ava_2076.
    PATRICi35424891. VBIAnaVar43351_3000.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000117 Genomic DNA. Translation: ABA21698.1 .
    RefSeqi YP_322593.1. NC_007413.1.

    3D structure databases

    ProteinModelPortali Q3MBD8.
    SMRi Q3MBD8. Positions 8-411.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 240292.Ava_2076.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA21698 ; ABA21698 ; Ava_2076 .
    GeneIDi 3680549.
    KEGGi ava:Ava_2076.
    PATRICi 35424891. VBIAnaVar43351_3000.

    Phylogenomic databases

    eggNOGi COG0112.
    HOGENOMi HOG000239404.
    KOi K00600.
    OMAi CAYVNVQ.
    OrthoDBi EOG6Z0QB2.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    UPA00288 ; UER01023 .
    BioCyci AVAR240292:GCY3-2099-MONOMER.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPi MF_00051. SHMT.
    InterProi IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view ]
    PANTHERi PTHR11680. PTHR11680. 1 hit.
    Pfami PF00464. SHMT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000412. SHMT. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00096. SHMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Anabaena variabilis ATCC 29413."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29413 / PCC 7937.

    Entry informationi

    Entry nameiGLYA_ANAVT
    AccessioniPrimary (citable) accession number: Q3MBD8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: October 25, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3