Reviewed, UniProtKB/Swiss-Prot Q3MB99 (SYFA_ANAVT)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Ava_2115

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_0000231959
Sites
	Metal binding	257	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MB99-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 15E63CCC5DB6723A	FASTA	330	37,120
	10 20 30 40 50 60 MTSNLEAQLL ALRQEGEQAI AAADTLERLE ELRVSYLGKK GQLGALLRSM GQMSAEERPK 70 80 90 100 110 120 IGAIANTVKE ALQASLDKQR ESLESAQIQA QLDAETLDVT MPGIYKPQGR IHPLNGIIDR 130 140 150 160 170 180 ALDVFVGLGY TVAQGLEMET DYYNFEALNT PPDHPARDMQ DTFYLPDGNL LRTHTSSVQI 190 200 210 220 230 240 RYMEKEEPPI HIVAPGRVYR RDNVDATHSA VFHQIELLAI DEGLTFTDLK GTIKVFLQAM 250 260 270 280 290 300 FGDLPIRFRA SYFPFTEPSA EVDLQWNGRW LEVMGCGMVD PNVMKSVGYD PEIYTGFAAG 310 320 330 FGVERFAMVL HQIDDIRRLY ASDLRFLRQF

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA21737.1.
RefSeq	YP_322632.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3680373.
GenomeReviews	Gene locus Ava_2115 in contig CP000117_GR.
KEGG	ava:Ava_2115.
NMPDR	fig|240292.3.peg.1714.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MB99.
OMA	Q3MB99. FRASYFP.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_2115-MON.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR002319. Phe-tRNA-synth_IIc-rel. IPR004529. Phe-tRNA-synth_IIc_asu. IPR018157. Phe-tRNA-synth_IIc_C. IPR004188. Phe-tRNA_synth_II_N. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_ANAVT

Accession

Primary (citable) accession number: Q3MB99

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)