Reviewed, UniProtKB/Swiss-Prot Q3MAV1 (PYRG_ANAVT)

Names and origin

Protein names

Recommended name:
    CTP synthase
    EC=6.3.4.2
Alternative name(s):
    UTP--ammonia ligase
    CTP synthetase

Gene names

Name:	pyrG
Ordered Locus Names:	Ava_2267

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity.
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	CTP synthase HAMAP MF_01227		PRO_0000266052
Regions
	Domain	292 – 534	243	Glutamine amidotransferase type-1
	Region	1 – 254	254	Aminator domain HAMAP MF_01227
Sites
	Active site	381	1	Nucleophile By similarity
	Active site	507	1	By similarity
	Active site	509	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MAV1-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 2F34AAF699AA7977	FASTA	545	60,341
	10 20 30 40 50 60 MTKFIFVTGG VVSSIGKGIV AASLGRLLKS RDYSVSILKL DPYINIDPGT MSPFQHGEVF 70 80 90 100 110 120 VTQDGAETDL DLGHYERFTD TSMSRLNSVT TGSIYQAVIM RERRGDYNGG TVQVIPHITN 130 140 150 160 170 180 EIKERILSVA KETNPSVVIT EIGGTVGDIE SLPFLEAIRQ LRKQVGRQNV LYMHVTLMPY 190 200 210 220 230 240 IASAGEMKTK PTQHSVKELR SIGIQPDILV CRSDRPIPRG LKQKLSEFCD VPVECVITSQ 250 260 270 280 290 300 DARSIYEVPL ILEGEGLAEQ TLKLLQMEQR QPNLEKWQAM VQGLYSPKHT VEIAIVGKYV 310 320 330 340 350 360 SLGDAYLSVV EALRHAAIAT QGDLQLRWIN SEDLETQAPE TYLAGVDGIV VPGGFGTRGV 370 380 390 400 410 420 DGKIAAIKYA RDRQIPFLGL CLGMQCSVIE WARNVGGLAG ANSAEFDSTT KYPVINLLPE 430 440 450 460 470 480 QQDVVDLGGT MRLGVYPCHI LPNTLASKLY QAEIIQERHR HRYEFNNDYR QLLLDSGYVI 490 500 510 520 530 540 SGTSPDGRLV EIVEYPQHPF FISCQFHPEF QSRPNTPHPL FTGFVQAAIA QSHPTANFQT PVKVS

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA21885.1.
RefSeq	YP_322780.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3679012.
GenomeReviews	Gene locus Ava_2267 in contig CP000117_GR.
KEGG	ava:Ava_2267.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MAV1.
OMA	Q3MAV1. EFNNAYR.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_2267-MON.
Family and domain databases
HAMAP	MF_01227. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_ANAVT

Accession

Primary (citable) accession number: Q3MAV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)