Reviewed, UniProtKB/Swiss-Prot Q3MAQ7 (SYP_ANAVT)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Ava_2311

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 604	604	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_0000248639

Sequences

	Sequence		Length	Mass (Da)
	Q3MAQ7-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: BA6200BEB7384C11	FASTA	604	67,688
	10 20 30 40 50 60 MRLSQMLFVT LRDDPADAEI PSHKLLLRAG YIRRIGSGIY AYLPLMWRVL QKVSQIVREE 70 80 90 100 110 120 MNAAGAQECL LPQLQPSELW KESGRWDTYT KAEGIMFSLI DRREQQLGLG PTHEEVITAI 130 140 150 160 170 180 ARDMIRSYRQ LPLHLYQLQT KFRDEIRPRF GLMRGREFIM KDGYSFHVDE DSLKKTYQDM 190 200 210 220 230 240 YQAYSNMLRR AGLAFRPVEA DSGAIGGSGS TEFMVLAEAG EDEVLYTDDG KYAANVEKAV 250 260 270 280 290 300 SLPADAEPSQ FTAFEKRETP GTETIEKVTQ FLKASPTQIV KNVLYQTVYD NGVTVLVLVI 310 320 330 340 350 360 IRGDQEVNEV KLQNELTKLA PNYGAKAIIN LTVPSAENQQ TWTAKSLPLG YIAPDIADEY 370 380 390 400 410 420 IAANKQIHPK FVRFVDKTAV DLKNFITGAN EAGYHVVGAN WGEQFPLPEI VVDVRKARPG 430 440 450 460 470 480 DRAIHDSTQI LKSARGIEVG HIFQLGTKYS QALGATYTNE QGEEKPLVMG CYGVGVSRLA 490 500 510 520 530 540 QSAVEQSYDK DGIIWPVAIA PYHAIVTIPN INDAQQVEIA ERLYTELNQS GVETLLDDRN 550 560 570 580 590 600 ERAGVKFKDA DLIGIPYRIV TGRAITNGKV EIVERATRQS QEIPIDEVIT TLQQWIKAAI EQKN

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA21929.1.
RefSeq	YP_322824.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3MAQ7.
Genome annotation databases
GeneID	3678919.
GenomeReviews	Gene locus Ava_2311 in contig CP000117_GR.
KEGG	ava:Ava_2311.
NMPDR	fig|240292.3.peg.191.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MAQ7.
OMA	DFVLGPT
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_2311-MON.
Family and domain databases
HAMAP	MF_01569. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 2 hits. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ANAVT

Accession

Primary (citable) accession number: Q3MAQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	October 25, 2005
Last modified:	November 24, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)