Reviewed, UniProtKB/Swiss-Prot Q3MAP8 (MURC_ANAVT)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Ava_2320

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000242540
Regions
	Nucleotide binding	140 – 146	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3MAP8-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 52A6CC110CB43639	FASTA	494	53,541
	10 20 30 40 50 60 MNNSVDFGGR PFHFIGIGGI GMSALAYVLA KRQLPVSGSD LRPNHITRKL ESIGAHIFSR 70 80 90 100 110 120 QEASNLEFFG SKVSSTEIEL NTQEMFPLGK STLPQVVCST AINSNNLEYQ AAIELGCPIL 130 140 150 160 170 180 HRSDVLAALI NDYHSVAVAG THGKTTTSSM IGYMLLEAGL DPTIIVGGEV NAWEGNARLG 190 200 210 220 230 240 QSPYLVAEAD ESDGSLVKHA PEIGIITNIE LDHPDHYDTL EEVVDIFQTF AKGCKTLIGS 250 260 270 280 290 300 VDCATVRELL RTAASDRQQP TITYSLHQDT EADYTVTNID CRADGTTALV WEKGKALGVL 310 320 330 340 350 360 KLKLLSRHNL SNALAAVAVG RLVGLEFGEI AKGIAGFEGA RRRFEFRGEV DGITFIDDYA 370 380 390 400 410 420 HHPSEIRATL AAARLQARPG QRVVAIFQPH RYSRTLTFLE EFSESFSHAD LVVLTDIYSA 430 440 450 460 470 480 GEPNLGLISG EQLAEKIAQE HPQVVYQPTL STVCEYLLKN LRPGDLALFL GAGNLNQAIP 490 EIITTLCEPA TATL

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA21938.1.
RefSeq	YP_322833.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3683590.
GenomeReviews	Gene locus Ava_2320 in contig CP000117_GR.
KEGG	ava:Ava_2320.
NMPDR	fig|240292.3.peg.199.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MAP8.
OMA	Q3MAP8. EWMVVEA.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_2320-MON.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ANAVT

Accession

Primary (citable) accession number: Q3MAP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)