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Protein

dITP/XTP pyrophosphatase

Gene

Ava_2368

Organism
Anabaena variabilis (strain ATCC 29413 / PCC 7937)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

A nucleoside triphosphate + H2O = a nucleotide + diphosphate.SAAS annotation
ITP + H2O = IMP + diphosphate.UniRule annotation
XTP + H2O = XMP + diphosphate.UniRule annotation
dITP + H2O = dIMP + diphosphate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • Mn2+SAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39MagnesiumUniRule annotation1
Active sitei68Proton acceptorUniRule annotation1
Metal bindingi68MagnesiumUniRule annotation1
Binding sitei69Substrate; via amide nitrogenUniRule annotation1
Binding sitei170SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported
Biological processNucleotide metabolismUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotationSAAS annotation, ManganeseSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciAVAR240292:G7WH-5827-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:Ava_2368Imported
OrganismiAnabaena variabilis (strain ATCC 29413 / PCC 7937)Imported
Taxonomic identifieri240292 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena
Proteomesi
  • UP000002533 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi240292.Ava_2368.

Structurei

3D structure databases

ProteinModelPortaliQ3MAK0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 14Substrate bindingUniRule annotation6
Regioni147 – 150Substrate bindingUniRule annotation4
Regioni175 – 176Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4108V82. Bacteria.
COG0127. LUCA.
HOGENOMiHOG000293319.
KOiK02428.
OMAiTHNPGKV.
OrthoDBiPOG091H02BP.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3MAK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKILVVATS NSGKLREMQA YLANTDWKLT LKPPELDVEE TGDTFAANAC
60 70 80 90 100
LKASEVAKAT GNWAIADDSG LQVDALNGVP GVYSARYGKT DSERISRLLK
110 120 130 140 150
ELDGEVNRKA QFVCVVAIAS PDGAITLQAE GICRGEILHA PRGSGGFGYD
160 170 180 190
PIFYVTEKQL TFAEMTPELK KSVSHRGKAF AALLPKMATV LSSSSE
Length:196
Mass (Da):20,985
Last modified:October 25, 2005 - v1
Checksum:i15C8CF4D829CEA08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000117 Genomic DNA. Translation: ABA21986.1.

Genome annotation databases

EnsemblBacteriaiABA21986; ABA21986; Ava_2368.
KEGGiava:Ava_2368.
PATRICi35425561. VBIAnaVar43351_3330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000117 Genomic DNA. Translation: ABA21986.1.

3D structure databases

ProteinModelPortaliQ3MAK0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi240292.Ava_2368.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA21986; ABA21986; Ava_2368.
KEGGiava:Ava_2368.
PATRICi35425561. VBIAnaVar43351_3330.

Phylogenomic databases

eggNOGiENOG4108V82. Bacteria.
COG0127. LUCA.
HOGENOMiHOG000293319.
KOiK02428.
OMAiTHNPGKV.
OrthoDBiPOG091H02BP.

Enzyme and pathway databases

BioCyciAVAR240292:G7WH-5827-MONOMER.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ3MAK0_ANAVT
AccessioniPrimary (citable) accession number: Q3MAK0
Entry historyiIntegrated into UniProtKB/TrEMBL: October 25, 2005
Last sequence update: October 25, 2005
Last modified: April 12, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.