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Q3MA89 (TRMFO_ANAVT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Synonyms:gid
Ordered Locus Names:Ava_2482
OrganismAnabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]
Taxonomic identifier240292 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037

Cofactor

FAD By similarity. HAMAP MF_01037

Subcellular location

Cytoplasm By similarity HAMAP MF_01037.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionflavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP MF_01037
PRO_1000084282

Regions

Nucleotide binding10 – 156FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3MA89 [UniParc].

Last modified October 25, 2005. Version 1.
Checksum: 2BE00125BED6D8EF

FASTA43848,219
        10         20         30         40         50         60 
MDQQPIQVIG GGLAGTEAAW QIAQAGVPVI LHEMRPKRFS PAHHTEHLAE LVCSNSFGAM 

        70         80         90        100        110        120 
ASDRAAGLLH EELRQLGSVV IAKADEHAVP AGGALAVDRG QFGQDLTQTL ASHPLVEFRR 

       130        140        150        160        170        180 
SEVPAIPEGI VVLATGPLTS PDLAADLHRF TGMEYMSFFD AASPIIVGDS INRDIAFMAS 

       190        200        210        220        230        240 
RYDKGEAAYL NCPMNKEQYL RFREELCKAE QTELKDFERE TAKFFEACLP IEELAQRGED 

       250        260        270        280        290        300 
TMRYGPVKPV GLSDSRTGER PYAVVQLRQE DKAGQLWNMV GFQTNLRWGE QKRVFQMIPG 

       310        320        330        340        350        360 
LEKAEFVRLG VMHRNTFINA PQLMLPTLQF KQRPTLLAAG QLIGTEGYTA AAAGGWLAGT 

       370        380        390        400        410        420 
NAARLALGKE PLALPPTTML GALLEFISSA SPKHFQPMPP NFGILPDLGM KIKSKPERYG 

       430 
RYRDRSLADL SSWKHNLN

« Hide

References

[1]"Complete sequence of Anabaena variabilis ATCC 29413."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29413 / PCC 7937.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000117 Genomic DNA. Translation: ABA22097.1.
RefSeqYP_322992.1. NC_007413.1.

3D structure databases

ProteinModelPortalQ3MA89.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ3MA89.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3682833.
GenomeReviewsGene locus Ava_2482 in contig CP000117_GR.
KEGGava:Ava_2482.
NMPDRfig|240292.3.peg.1737.
PATRIC35425813. VBIAnaVar43351_3456.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1206.
HOGENOMHBG285041.
OMARFAGQIT.
PhylomeDBQ3MA89.
ProtClustDBPRK05335.

Enzyme and pathway databases

BioCycAVAR240292:AVA_2482-MONOMER.

Family and domain databases

HAMAPMF_01037. TrmFO.
[Tree]
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
KOK04094.
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. Gid_trmFO. 1 hit.
PROSITEPS01280. GIDA_1. False negative.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMFO_ANAVT
AccessionPrimary (citable) accession number: Q3MA89
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 25, 2005
Last modified: January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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