Reviewed, UniProtKB/Swiss-Prot Q3MA89 (TRMFO_ANAVT)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Synonyms:	gid
Ordered Locus Names:	Ava_2482

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_1000084282
Regions
	Nucleotide binding	10 – 15	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3MA89-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 2BE00125BED6D8EF	FASTA	438	48,219
	10 20 30 40 50 60 MDQQPIQVIG GGLAGTEAAW QIAQAGVPVI LHEMRPKRFS PAHHTEHLAE LVCSNSFGAM 70 80 90 100 110 120 ASDRAAGLLH EELRQLGSVV IAKADEHAVP AGGALAVDRG QFGQDLTQTL ASHPLVEFRR 130 140 150 160 170 180 SEVPAIPEGI VVLATGPLTS PDLAADLHRF TGMEYMSFFD AASPIIVGDS INRDIAFMAS 190 200 210 220 230 240 RYDKGEAAYL NCPMNKEQYL RFREELCKAE QTELKDFERE TAKFFEACLP IEELAQRGED 250 260 270 280 290 300 TMRYGPVKPV GLSDSRTGER PYAVVQLRQE DKAGQLWNMV GFQTNLRWGE QKRVFQMIPG 310 320 330 340 350 360 LEKAEFVRLG VMHRNTFINA PQLMLPTLQF KQRPTLLAAG QLIGTEGYTA AAAGGWLAGT 370 380 390 400 410 420 NAARLALGKE PLALPPTTML GALLEFISSA SPKHFQPMPP NFGILPDLGM KIKSKPERYG 430 RYRDRSLADL SSWKHNLN

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA22097.1.
RefSeq	YP_322992.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3682833.
GenomeReviews	Gene locus Ava_2482 in contig CP000117_GR.
KEGG	ava:Ava_2482.
NMPDR	fig|240292.3.peg.1737.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MA89.
OMA	Q3MA89. MKPVGLT.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_2482-MON.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR004417. Gid. IPR002218. GIDA-rel. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_ANAVT

Accession

Primary (citable) accession number: Q3MA89

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)