Reviewed, UniProtKB/Swiss-Prot Q3M9Q2 (RNPH_ANAVT)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	Ava_2671

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	Ribonuclease PH HAMAP MF_00564		PRO_1000024773

Sequences

	Sequence		Length	Mass (Da)
	Q3M9Q2-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 0C90C04AB939A629	FASTA	247	27,081
	10 20 30 40 50 60 MVWQRPDGRK PYELRPINFH TKFTRFAPGS VLTICGETKV LCTVSVAESV PKFLAGSGKG 70 80 90 100 110 120 WLTAEYRMLP SATQQRHERE LLKLSGRTQE IQRLIGRSLR AAIDFEALGE RTLTVDADVL 130 140 150 160 170 180 QADAGTRTAA ITGGFVALAE AISQLLQRGV LERSPLCGQV AAVSVGLLEQ EAYLDLNYIE 190 200 210 220 230 240 DVAATVDFNV VMNKNLGIIE VQGTAEEGSF SRTQLNQLLD CAETGIQQLL IAQQKAITDW DELFVGK

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA22284.1.
RefSeq	YP_323179.1.
3D structure databases
HSSP	HSSP built from PDB template 2C39 based on UniProtKB Q9UXC2.
SMR	Q3M9Q2. Positions 4-239.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3M9Q2.
Genome annotation databases
GeneID	3682032.
GenomeReviews	Gene locus Ava_2671 in contig CP000117_GR.
KEGG	ava:Ava_2671.
NMPDR	fig|240292.3.peg.945.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	Q3M9Q2.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_2671-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_ANAVT

Accession

Primary (citable) accession number: Q3M9Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2005
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)